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991.
992.
Ran Nakashima Yoshitaka Imura Yuji Hosono Minae Seto Akihiro Murakami Kizuku Watanabe Tomohiro Handa Michiaki Mishima Michito Hirakata Tsutomu Takeuchi Keishi Fujio Kazuhiko Yamamoto Hitoshi Kohsaka Yoshinari Takasaki Noriyuki Enomoto Takafumi Suda Kingo Chida Shu Hisata Toshihiro Nukiwa Tsuneyo Mimori 《PloS one》2014,9(1)
993.
The population dynamics of Marginopora kudakajimensis Gudmundsson, a dinoflagellate endosymbiont-bearing soritid foraminifer, was studied in the Ryukyu Islands, the subtropical northwest Pacific. Macroalgal samples were collected monthly between November 1995 and November 1996 at a 1-m-deep lagoonal site colonized by this species. Monthly variations in the size–frequency distributions and population density indicate that this foraminiferal population replaces itself in one year. Asexual reproduction occurs twice a year, in late spring and winter; in late spring, some of the adult individuals reproduce by multiple fission simultaneously. As a result, two cohorts (a late spring and a winter cohort) are found in the population during a year. Life span of the former cohort is up to one year, while that of the latter cohort is up to six months. Megalospheric specimens comprise up to 99% of the population with a few microspheric individuals throughout the year. The life tables and survivorship curves revealed that size-specific mortality rates were very low during the first several size classes and increased thereafter, indicating low juvenile mortality with high mortality later in life. The carbonate production rate by this Marginopora population is approximately 5 kg CaCO3 m−2 yr−1, which is extremely higher than those reported for other larger foraminiferal species. 相似文献
994.
Sleep and Biological Rhythms - Physiologically, sleep is defined by electroencephalography, and typical REM and NREM sleep occur only in mammalian and avian species. But sleep-like inactive states... 相似文献
995.
996.
Yu Ichida Yuko Utsunomiya Junko Tomikawa Kazuhiko Nakabayashi Toshinori Sato Masafumi Onodera 《Bioscience, biotechnology, and biochemistry》2016,80(1):114-120
Expression of Moloney murine leukemia virus (MoMLV)-typed retroviral vectors is strictly suppressed in immature cells such as embryonic stem cells. The phenomenon known as gene silencing is primed by the sequence-specific binding of the zinc finger protein 809 (ZFP809) to the primer-binding site of the vectors. However, it has yet to be determined whether the ZFP809-mediated gene silencing is maintained over a long period. In this study, we established an experimental system that can monitor gene silencing during a long-term cell culture using flow cytometry technology combined with fluorescent reporters for the expression of ZFP809 and the transgene expression driven by the promoters of interest. Time-course analysis using our system revealed that ZFP809 maintains gene silencing effect even at a longtime period. Furthermore, our system was useful for the monitoring of ZFP809-mediated gene silencing regardless of the types of vectors and cell lines. 相似文献
997.
Kazuhiko S.K. Uchida Kentaro Takagaki Kazuki Kumada Youko Hirayama Tetsuo Noda Toru Hirota 《The Journal of cell biology》2009,184(3):383-390
The spindle assembly checkpoint (SAC) monitors the attachment of microtubules to the kinetochore and inhibits anaphase when microtubule binding is incomplete. The SAC might also respond to tension; however, how cells can sense tension and whether its detection is important to satisfy the SAC remain controversial. We generated a HeLa cell line in which two components of the kinetochore, centromere protein A and Mis12, are labeled with green and red fluorophores, respectively. Live cell imaging of these cells reveals repetitive cycles of kinetochore extension and recoiling after biorientation. Under conditions in which kinetochore stretching is suppressed, cells fail to silence the SAC and enter anaphase after a delay, regardless of centromere stretching. Monitoring cyclin B levels as a readout for anaphase-promoting complex/cyclosome activity, we find that suppression of kinetochore stretching delays and decelerates cyclin B degradation. These observations suggest that the SAC monitors stretching of kinetochores rather than centromeres and that kinetochore stretching promotes silencing of the SAC signal. 相似文献
998.
High bone mass gained by exercise in growing male mice is increased by subsequent reduced exercise. 总被引:3,自引:0,他引:3
Jian Wu Xin Xiang Wang Mitsuru Higuchi Kazuhiko Yamada Yoshiko Ishimi 《Journal of applied physiology》2004,97(3):806-810
Exercise-induced bone gains are lost if exercise ceases. Therefore, continued exercise at a reduced frequency or intensity may be required to maintain these benefits. In this study, we evaluated whether 4 wk of reduced exercise after 4 wk of running exercise in growing male mice results in the maintenance of high bone mass. Five-week-old mice were divided into the following groups: 1) baseline control; 2) 4-wk control; 3) 4-wk exercise; 4) 8-wk control; 5) 4-wk exercise followed by 4-wk cessation of training; and 6) 4-wk exercise followed by reduced exercise at half the frequency. The regimen consisted of exercise 6 days/wk, and the reduced exercise regimen consisted of running 3 days/wk on a treadmill for 30 min/day, at 12 m/min on a 10 degrees uphill slope. Running exercise significantly increased bone mineral density of the femur, periosteal mineral apposition rate, bone formation rate, percent labeled perimeter at the midfemur, and osteogenic activity of bone marrow cells. However, these parameters declined to the age-matched sedentary control after cessation of training. In contrast, the reduced exercise group had significantly higher mineral apposition rate compared with those of the sedentary control and cessation of training groups. Furthermore, bone mineral density for the reduced exercise group was significantly higher than those for the other groups. These results suggest that the high bone formation gained through exercise can be maintained, and bone mass was further increased by subsequent exercise even if the exercise frequency is reduced. 相似文献
999.
In the eastern Seto Inland Sea, Japan, phytoplankton abundance in the surface water has gradually declined, whereas Secchi depth has risen in recent years, particularly in offshore areas. Therefore, it may be hypothesized that phytoplankton dominate light attenuation in the offshore area, and that other constituents are less important. To test this hypothesis, we examined the roles of seawater, colored dissolved organic matter (CDOM), non-algal particles (tripton), and phytoplankton in the light attenuation at an offshore station of Harima Sound in the eastern Sea. The magnitude of light attenuation was then determined from the attenuation coefficient of photosynthetically available radiation (PAR) through the water column (K d). During a 13-month period, K d ranged from 0.179 to 0.507 m?1, with a mean of 0.262 m?1. The mean relative contributions of seawater (15%) and CDOM (13%) to K d were small, while the most dominant K d constituent was tripton (45%). The mean contribution of phytoplankton to K d (27%) was consequently less than that of tripton. However, 75% of the temporal variability in K d was attributed to phytoplankton, measured as chlorophyll a. Our results emphasize that the main component of light attenuation does not always govern the temporal variation of light attenuation in coastal regions. 相似文献
1000.