全文获取类型
收费全文 | 1082篇 |
免费 | 97篇 |
出版年
2022年 | 4篇 |
2021年 | 16篇 |
2020年 | 7篇 |
2019年 | 10篇 |
2018年 | 27篇 |
2017年 | 19篇 |
2016年 | 29篇 |
2015年 | 36篇 |
2014年 | 39篇 |
2013年 | 58篇 |
2012年 | 50篇 |
2011年 | 48篇 |
2010年 | 40篇 |
2009年 | 38篇 |
2008年 | 60篇 |
2007年 | 65篇 |
2006年 | 53篇 |
2005年 | 59篇 |
2004年 | 53篇 |
2003年 | 44篇 |
2002年 | 45篇 |
2001年 | 37篇 |
2000年 | 43篇 |
1999年 | 28篇 |
1998年 | 11篇 |
1997年 | 18篇 |
1996年 | 12篇 |
1995年 | 12篇 |
1994年 | 10篇 |
1993年 | 8篇 |
1992年 | 22篇 |
1991年 | 15篇 |
1990年 | 23篇 |
1989年 | 18篇 |
1988年 | 8篇 |
1987年 | 16篇 |
1986年 | 17篇 |
1985年 | 6篇 |
1984年 | 11篇 |
1983年 | 8篇 |
1982年 | 4篇 |
1980年 | 6篇 |
1979年 | 6篇 |
1977年 | 5篇 |
1976年 | 3篇 |
1974年 | 7篇 |
1973年 | 6篇 |
1972年 | 3篇 |
1969年 | 4篇 |
1966年 | 3篇 |
排序方式: 共有1179条查询结果,搜索用时 3 毫秒
11.
Binding and endocytosis of glycoproteins and neoglycoproteins by isolated rabbit hepatocytes. 下载免费PDF全文
D T Connolly R R Townsend K Kawaguchi M K Hobish W R Bell Y C Lee 《The Biochemical journal》1983,214(2):421-431
Rabbit hepatocytes were isolated by a collagenase perfusion technique, and used to study the binding and endocytosis of the glycoprotein, asialo-orosomucoid, and the neoglycoprotein, Gal39-bovine serum albumin. Both of these proteins contain exposed galactosyl residues, and were avidly bound by the lectin on the hepatic parenchymal cell surface. Steady state and kinetic experiments performed at 2 degrees C and at 37 degrees C revealed the presence of two apparent classes of binding sites totalling 4.7 X 10(5) sites/cell at 2 degrees C, and 6.3 X 10(5) sites/cell at 37 degrees C. At 37 degrees C, both classes of sites participated in internalization of bound ligand. The cells were capable of internalizing about 60 000 molecules/min per cell. The process appeared to be first-order, with a rate constant k = 0.098 min-1 and t1/2 = 7.1 +/- 0.6 min. Binding could be inhibited by galactose-containing compounds, EGTA, and by anti-(hepatic lectin) immunoglobulin G. The inhibition by antibody appeared to be reversible upon removal of antibody-containing medium. 相似文献
12.
13.
14.
Growth and death rates of aboveground plant parts were measured in a mature forest and four different-aged deciduous broadleaf forests regeneratede after clear-cutting, with special reference to rates for woody parts (stems and branches) of different diameters (ø) in rerms of the pipe model theory (Shinozaki et al., 1964). The total biomass increment of woody parts of trees higher than 1.3 m varied within a range of 2.1-4.6 ton ha?1 yr?1, the increase beingdue largely to the growth of canopy trees exposed to direct sunlight. Biomass increments of small (ø<1 cm) and medium (1≤ø<5 cm) woody parts were negligibly small except in the youngest forest, and changes in aboveground woody biomass with forest age after clear-cutting mainly resulted from accumulation of large (5 cm<ø) woody parts of canopy trees. Biomass loss of trees due to death and grazing increased with forest age from 4.0 to 8.3 ton ha?1 yr?1. Recovery of leaf and small wood falls was observed at the early stage of regeneration, while large wood falls increased during regeneration. Flower and fruit fall was markedly higher in the mature forest than in the other four forest types. Mortality of woody parts became higher with forest age and was 20, 5.0 and 0.46% yr?1 for small, medium and large parts, respectively, at the mature stage. Aboveground net production of the forest was in therange 7.6-13.3 ton ha?1 yr?1 with the undergrowth vegetation lower than 1.3 m being 0.4-1.4 ton ha?1 yr?1. Production recovered rapidly at an early stage of regeneration and was highest in mature forest. 相似文献
15.
Initiation of meiosis and sporulation in Saccharomyces cerevisiae requires a novel protein kinase homologue 总被引:5,自引:0,他引:5
16.
17.
Kawaguchi Masayoshi; Imaizumi-Anraku Haruko; Fukai Shungo; Syono Kunihiko 《Plant & cell physiology》1996,37(4):461-470
We studied the effects of several plant-growth regulators onthe induction of nodule-like structures on roots of Lotus japonicus,which has been proposed as a candidate for a leguminous plantfor molecular genetic analysis. Contrary to our expectations,the addition of gibberellin A3 (GA3) at concentrations of 10-4M to 10-4 M resulted in the formation of nodule-like structureson roots when seedlings were plated on nitrogen-free Fahraeusagar medium. GA4 also induced such outgrowths but was less activethan GA3. Application of an inhibitor of auxin transport, N-(1-naphthyl)-phthalamicacid (NPA) and of kinetin, which have been reported to inducepseudonodules in other legumes, had no effect on L. japonicus.Microscopic observations showed that GA3-induced nodule-likestructures were caused by cell divisions within the pericycleon the roots. In addition, the outgrowths elicited by GA3 couldbe completely suppressed by the addition of 15 mM potassiumnitrate or ammonium nitrate. These results show that the pericyclecells of the roots of L. japonicus are specifically sensitiveto gibberellins and that potential for cell division might bemodulated by nitrogen compounds. We also examined the effectsof ancymidol and uniconazole [S-3307; (E)-1-(4-chIorophenyl)-4,4-dimethyl-2-(1,2,4-triazol-1-yl)-1-penten-3-ol],two synthetic plant-growth retardants. Both compounds at 3 x10-5 M significantly increased the number of stunted lateralroots. The unusual branching could not be counteracted by theexogenous addition of GA3 but by 10-6 M brassinolide. We discussthe physiological role of brassinolide in the initiation oflateral roots. (Received August 4, 1995; Accepted March 11, 1996) 相似文献
18.
Because of the importance of indole-3-acetic acid (IAA) in thegrowth and development of plants, extensive studies of the biosynthesisof IAA have been performed during the four decades since thediscovery of IAA as a plant hormone. The pathway for the biosynthesisof IAA in plants remains, however, to be unelucidated, eventhough studies within the past decade have revealed unexpectedaspects of such biosynthesis. By contrast, two pathways to IAAhave been characterized in bacteria at the molecular level:the indole-3-acetamide (IAM) pathway (L-tryptophan 相似文献
19.
Kenichiro Nakashima Naotaka Kuroda Shinki Kawaguchi Mitsuhiro Wada Shuzo Akiyama 《Luminescence》1995,10(3):185-191
A sensitive peroxyoxalate chemiluminescent (PO-CL) assay for activities of oxidases (uricase, choline oxidase, cholesterol oxidase and xanthine oxidase) which catalyse a formation of hydrogen peroxide was developed using 4,4′-oxalyl-bis[(trifluoromethylsulphonyl)imino]trimethylene-bis(4-methylmorpholinium)trifluoromethanesulphonate as a chemiluminogenic reagent and 2,4,6,8-tetramorpholinopyrimido[5,4-d]pyrimidine as a fluorophore. The standard curve for hydrogen peroxide was linear over the range 1 × 10?7-1 × 10?4 mol/L. Relative standard deviations for oxidase assays were 5.1–12.7% (n = 10). Detection limits were 1 × 10?3 U/mL for uricase, 5 × 10?4 U/mL for choline oxidase, 5 × 10?3 U/mL for cholesterol oxidase and 5 × 10?4 U/mL xanthine oxidase (sample to blank ratio, 3). 相似文献
20.