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Takayo Ota Haruka Asahina Se-Hyung Park Qing Huang Takashi Minegishi Nelly Auersperg Peter CK Leung 《Reproductive biology and endocrinology : RB&E》2008,6(1):49
Background
HOX cofactors enhance HOX binding affinities and specificities and increase HOX's unique functional activities. The expression and the regulation of HOX cofactors in human ovaries are unknown. 相似文献373.
Ikki Horiguchi Fuad Gandhi Torizal Hotaka Nagate Haruka Inose Kousuke Inamura Osamu Hirata Hisato Hayashi Masato Horikawa Yasuyuki Sakai 《Biotechnology progress》2021,37(2):e3100
Suspension culture is an important method used in the industrial preparation of pluripotent stem cells (PSCs), for regenerative therapy and drug screening. Generally, a suspension culture requires agitation to keep PSC aggregates suspended and to promote mass transfer, but agitation also causes cell damage. In this study, we investigated the use of a Bingham plastic fluid, supplemented with a polysaccharide-based polymer, to preserve PSCs from cell damage in suspension culture. Rheometric analysis showed that the culture medium gained yield stress and became a Bingham plastic fluid, after supplementation with the polymer FP003. A growth/death analysis revealed that 2 days of aggregate formation and 2 days of suspension in the Bingham plastic medium improved cell growth and prevented cell death. After the initial aggregation step, whereas strong agitation (120 rpm) of a conventional culture medium resulted in massive cell death, in the Bingham plastic fluid we obtained the same growth as the normal culture with optimal agitation (90 rpm). This indicates that Bingham plastic fluid protected cells from shear stress in suspension culture and could be used to enhance their robustness when developing a large-scale. 相似文献
374.
Haruka Suzuki Tomoya Aoki Eri Inomata Yukio Agatsuma Masakazu N. Aoki 《Phycological Research》2021,69(1):3-11
We conducted a 5‐year monitoring survey of a population of Eisenia bicyclis kelp at a coastal area that suffered subsidence from the 2011 earthquake and the subsequent breakwater restoration work. Due to subsidence, the former intertidal area sank to the subtidal zone, therefore, the density of E. bicyclis increased because of many recruits. However, after the restoration work, the area harboring the recruits was destroyed and landfilled. In an area that sank below the pre‐subsidence border of the kelp distribution, recruits were rare. Furthermore, an indirect effect of the restoration work was also observed on recruitment in the intact offshore kelp zone. Our results showed that the breakwater restoration work following subsidence had negative effects, not only to the density but also to the age structure of the E. bicyclis population. 相似文献
375.
Kakeru Suzuki Haruka Inoue Satoshi Matsuoka Ryugo Tero Ayumi Hirano-Iwata 《Bioscience, biotechnology, and biochemistry》2020,84(10):2028-2036
ABSTRACT Eukaryotic in vitro translation systems require large numbers of protein and RNA components and thereby rely on the use of cell extracts. Here we established a new in vitro translation system based on rice callus extract (RCE). We confirmed that RCE maintains its initial activity even after five freeze-thaw cycles and that the optimum temperature for translation is around 20°C. We demonstrated that the RCE system allows the synthesis of hERG, a large membrane protein, in the presence of liposomes. We also showed that the introduction of a bicistronic mRNA based on 2A peptide to RCE allowed the production of two distinct proteins from a single mRNA. Our new method thus facilitates laboratory-scale production of cell extracts, making it a useful tool for the in vitro synthesis of proteins for biochemical studies. 相似文献
376.
Maruyama Takuma Kamihama Haruka Watanabe Mutsuto Matsuo Toshiki Matsuda Kenshiro Tanaka Akane 《Bioscience, biotechnology, and biochemistry》2018,82(7):1101-1106
The chondroprotective effect of olive leaf extract (OLE) on knee osteoarthritis (OA) was studied with STR/ort mice (n = 5). OLE was administrated with a dosage of 100 mg/kg for 8 weeks and the OA severity score of hind limb knee joints was then measured. The Mankin scores of the knee joints of the non-OA control group, OA control group and OLE-treated group were 3.50, 11.13 and 7.20, respectively. This suggests that oral OLE supplements help prevent cartilage degeneration in STR/ort mice. In vitro, the synthesis of high molecular weight hyaluronan in synovial cells (HIG-82) was increased by OLE stimulation. This suggests that OLE modulates hyaluronan metabolism in synovial cells and improves OA symptoms. Our findings indicate that OLE intake inhibits cartilage destruction by increasing high molecular weight hyaluronan and thus preventing OA progress. 相似文献
377.
Sumire Hoashi-Takiguchi Hirobumi Morisaki Momoe Itsumi Mariko Kikuchi Haruka Fukamachi Mie Kurosawa Michi Yamada Noriyuki Suzuki Hirotaka Kuwata 《Biochemistry and Biophysics Reports》2022
Recently, it has been demonstrated that dysbiosis, an alteration in commensal microflora composition, is intimately involved in the onset of a variety of diseases. It is becoming increasingly evident that the composition of commensal microflora in the oral cavity is closely connected to oral diseases, such as periodontal disease, and systemic diseases, such as inflammatory bowel disease. Next-generation sequencing techniques are used as a method to examine changes in bacterial flora, but additional analytical methods to assess bacterial flora are needed to understand bacterial activity in more detail. In addition, the oral environment is unique because of the role of secretory antibodies contained in saliva in the formation of bacterial flora. The present study aimed to develop a new method for evaluating the compositional change of microbiota using flow cytometry (FCM) with specific antibodies against the bacterial surface antigen, as well as salivary antibodies. Using specific antibodies against Streptococcus mutans, a causative agent of dental caries, and human IgA, bacterial samples from human saliva were analyzed via FCM. The results showed that different profiles could be obtained depending on the oral hygiene status of the subjects. These results suggest that changes in the amount and type of antibodies that bind to oral bacteria may be an indicator for evaluating abnormalities in the oral flora. Therefore, the protocol established in this report could be applied as an evaluation method for alterations in the oral microbiota. 相似文献
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Haruka Ishihara Kohei Ogura Tohru Miyoshi-Akiyama Masahiko Nakamura Hiroyasu Kaya Shigefumi Okamoto 《Microbiology and immunology》2020,64(2):113-122
Streptococcus dysgalactiae subsp. equisimilis (SDSE) causes severe invasive streptococcal infections, especially in elderly people. Between 2013 and 2018, 88 streptococci were isolated from clinical blood culture in a hospital in Toyama prefecture, Japan. The collection included six Group A SDSE (ASD) strains, which are rarely isolated. Multilocus sequence typing categorized five of the six strains into ST128 and the remaining strain into a new type. Maximum-likelihood phylogenetic analysis revealed that the six ASD strains had highly similar genome sequences. Bayesian analysis indicated that the most recent common ancestor of the strains appeared 39 years ago. The ASD strains possessed carbohydrate synthase genes that are conserved in Streptococcus pyogenes strains, whereas one strain featured a different arrangement of the gene cluster. The carbohydrate synthase genes varied by Lancefield type (A, C, and G). 相似文献
380.
Arai Haruka Yanagiura Kazuya Toyama Yuko Morohashi Kengo 《Journal of plant research》2019,132(2):197-209
Journal of Plant Research - The evolution of plants on land required adaptation to UV radiation and dry environments, and involved the appearance and/or rewiring of genetic connections, known as... 相似文献