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71.
72.
M Enamul Kabir Senthilkumar Krishnaswamy Masahiko Miyamoto Yasuhiro Furuichi Tadazumi Komiyama 《BMC biotechnology》2009,9(1):99-16
Background
Phage-display panning is an integral part of biomedical research. Regular panning methods are sometimes complicated by inefficient detachment of the captured phages from the antigen-coated solid supports, which prompted us to modify. Here, we produce an efficient antigen-specific single chain fragment variable (scFv) antibody by using a target-related molecule that favored selection ofrecombinant antibodies. 相似文献73.
Dinakaran M Senthilkumar P Yogeeswari P China A Nagaraja V Sriram D 《Bioorganic & medicinal chemistry》2008,16(6):3408-3418
Various 2-(sub)-3-fluoro/nitro-5,12-dihydro-5-oxobenzothiazolo[3,2-a]quinoline-6-carboxylic acid derivatives were synthesized from 2-aminothiophenol by a five-step reaction, evaluated for in-vitro and in-vivo antimycobacterial activities against Mycobacterium tuberculosis H37Rv (MTB), multi-drug resistant Mycobacterium tuberculosis (MDR-TB), and Mycobacterium smegmatis (MC2), and also tested for the ability to inhibit the supercoiling activity of DNA gyrase from M. smegmatis. Among the thirty-four synthesized compounds, 2-(3-(diethylcarbamoyl)piperidin-1-yl)-)-3-fluoro-5,12-dihydro-5-oxobenzothiazolo[3,2-a]quinoline-6-carboxylic acid (7l) was found to be the most active compound in vitro with MIC of 0.18 and 0.08 microM against MTB and MTR-TB, respectively. Compound 7l was found to be 2 and 570 times more potent than isoniazid against MTB and MDR-TB, respectively. In the in-vivo animal model 7l decreased the bacterial load in lung and spleen tissues with 2.78 and 3.12-log10 protections, respectively, at the dose of 50 mg/kg body weight. 相似文献
74.
Optimization of media composition for Nattokinase production by Bacillus subtilis using response surface methodology 总被引:3,自引:0,他引:3
Deepak V Kalishwaralal K Ramkumarpandian S Babu SV Senthilkumar SR Sangiliyandi G 《Bioresource technology》2008,99(17):8170-8174
Response surface methodology and central composite rotary design (CCRD) was employed to optimize a fermentation medium for the production of Nattokinase by Bacillus subtilis at pH 7.5. The four variables involved in this study were Glucose, Peptone, CaCl(2), and MgSO(4). The statistical analysis of the results showed that, in the range studied; only peptone had a significant effect on Nattokinase production. The optimized medium containing (%) Glucose: 1, Peptone: 5.5, MgSO(4): 0.2 and CaCl(2): 0.5 resulted in 2-fold increased level of Nattokinase (3194.25U/ml) production compared to initial level (1599.09U/ml) after 10h of fermentation. Nattokinase production was checked with fibrinolytic activity. 相似文献
75.
This paper describes the hitherto unreported aspects of orchid mycorrhizae. The host cells harbour upto 4 generations of fungal
pelotons which are formed after each peloton is digested. There are two types of hyphae in a host cell, one forming the pelotons,
and the other which lies close to the host cell wall and separated from the former by a callosic wall. The later, called non-pelotonic
hyphae form the fresh peloton when the former is digested. Consequently, there are also cytochemical differences between these
two types of hyphae.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
76.
Biocatalysts are intrinsically reactive and hence their operational stability is of vital significance for any bioprocess. The setback in biocatalyst stability has been tackled from diverse prospects. Inherently, stable biocatalysts are markedly realized and a regular attempt is being made to seek out new organisms that harbor them. Here, we analyzed the industrial biocatalyst lipase A (Native) of Bacillus subtilis and its six thermostable mutants (2M, 3M, 4M, 6M, 9M and 12M) computationally using conformational sampling technique. Consequently, the various structural events deciphering thermostability like root mean square deviation, root mean square fluctuation, radius of gyration and polar surface area showed mutant 12M to be highly stable with statistical validation. Besides, static model analysis involving intra-molecular interactions, secondary structure, solvent accessibility, hydrogen bond pattern, simulated thermal denaturation and desolvation energy also supported 12M comparatively. Of note, the presence of high secondary structural rigidity and hydrogen bonds increased thermostability and functionality of 12M, thus selecting it as a best template for designing thermostable lipases in future. Also, this study has a significant implication toward a better understanding of conformational sampling in enzyme catalysis and enzyme engineering. 相似文献
77.
M. Subathra T. M. A. Senthilkumar P. Ramadass V. Ramaswamy 《World journal of microbiology & biotechnology》2011,27(5):1077-1082
Leptospirosis is one of the diseases with economic impact, so its diagnosis and serosurveillance are very important for any
control program. Many tests have been used in the field as screening tests of leptospirosis. The aim of this study was to
evaluate the efficacy of combined recombinant antigens (rLipL41 + rLipL32) for serodiagnosis of canine leptospirosis and to
compare the efficacy of IgG Enzyme linked immunosorbent assay and Latex agglutination test with standard MAT. A total of 533
canine serum samples were subjected to IgG-ELISA and LAT using recombinant LipL41 and LipL32 antigens in a single and in a
combinations which were compared with standard MAT. The potential diagnostic cocktails of combined recombinant antigens (rLipL31 + rLipL41)
developed in this study showed higher sensitivity and specificity in IgG ELISA and LAT (94.84, 88.50%; 98.21, 97.7%) when
compared to the use of single recombinant antigen in this study. Results indicated that the both assays can be easily performed,
and avoids the risk of infection in laboratory workers, and it seems to be a practical and suitable tool for serodiagnosis
of leptospirosis. 相似文献
78.
We describe the catalytic voltammograms of the periplasmic arsenite oxidase (Aio) from the chemolithoautotrophic bacterium Rhizobium sp. str. NT-26 that oxidizes arsenite to arsenate. Electrochemistry of the enzyme was accomplished using its native electron transfer partner, cytochrome c552 (cyt c552), as a mediator. The protein cyt c552 adsorbed on a mercaptoundecanoic acid (MUA) modified Au electrode exhibited a stable, reversible one-electron voltammetric response at + 275 mV vs NHE (pH 6). In the presence of arsenite and Aio the voltammetry of cyt c552 is transformed from a transient response to an amplified sigmoidal (steady state) wave consistent with an electro-catalytic system. Digital simulation was performed using a single set of parameters for all catalytic voltammetries obtained at different sweep rates and various substrate concentrations. The obtained kinetic constants from digital simulation provide new insight into the kinetics of the NT-26 Aio catalytic mechanism. 相似文献
79.
Ravichandran Senthilkumar Rahul Chandran Thangaraj Parimelazhagan 《Saudi Journal of Biological Sciences》2014,21(5):409-416
Rhodiola imbricata is a perennial herb of the family Crassulaceae, which has significant traditional usage as medicine and is also known to biosynthesize phytochemicals such as flavonoids, coumarins and phenyl glycosides. The present investigation was aimed to estimate the hepatoprotective activity of R. imbricata rhizome acetone extract against paracetamol (2 g/kg) induced liver toxicity. Paracetamol was administered to induce hepatic damage in Wistar rats. 200 and 400 mg/kg doses of rhizome acetone extract and silymarin (25 mg/kg) were used as treatment groups. The blood samples were analyzed for biochemical markers of hepatic injury and tissue samples were subjected for estimation of liver antioxidants and histopathological studies. Analysis of the extract treated rats (400 mg/kg) showed an elevation of superoxide dismutase (0.326 units/min/mg protein), catalase (185.03 μmole of H2O2 consumed/min/mg protein), glutothione peroxidase (19.26 mg GSH consumed/min/mg protein) and reduced glutathione (16.2 μmole of GSH/mg protein). Moreover, the biochemical parameters in serum like alkaline phosphatase, serum glutamic oxaloacetic transaminase (SGOT), serum glutamic pyruvic transaminase (SGPT) and lipid profiles were also improved in treated groups compared to the control. The oral administration of different doses of rhizome acetone extract significantly protected the hepatic cells from damage. The hematological and biochemical parameters were also normal in extract treated rats compared to the control and standard (silymarin) groups. The HPLC analysis revealed the presence of some important phenolic compounds which could be responsible for the hepatoprotective activity. This study proved that R. imbricata could be taken as a good natural source of the hepatoprotective agent. 相似文献