全文获取类型
收费全文 | 7505篇 |
免费 | 667篇 |
国内免费 | 4篇 |
出版年
2023年 | 53篇 |
2022年 | 67篇 |
2021年 | 274篇 |
2020年 | 147篇 |
2019年 | 177篇 |
2018年 | 200篇 |
2017年 | 161篇 |
2016年 | 339篇 |
2015年 | 511篇 |
2014年 | 521篇 |
2013年 | 533篇 |
2012年 | 757篇 |
2011年 | 650篇 |
2010年 | 378篇 |
2009年 | 366篇 |
2008年 | 492篇 |
2007年 | 419篇 |
2006年 | 377篇 |
2005年 | 291篇 |
2004年 | 293篇 |
2003年 | 265篇 |
2002年 | 242篇 |
2001年 | 53篇 |
2000年 | 31篇 |
1999年 | 38篇 |
1998年 | 60篇 |
1997年 | 31篇 |
1996年 | 24篇 |
1995年 | 27篇 |
1994年 | 24篇 |
1993年 | 29篇 |
1992年 | 26篇 |
1991年 | 21篇 |
1990年 | 17篇 |
1989年 | 26篇 |
1988年 | 13篇 |
1987年 | 13篇 |
1986年 | 11篇 |
1985年 | 12篇 |
1984年 | 15篇 |
1983年 | 24篇 |
1982年 | 8篇 |
1981年 | 16篇 |
1980年 | 13篇 |
1979年 | 11篇 |
1978年 | 15篇 |
1977年 | 12篇 |
1976年 | 9篇 |
1975年 | 9篇 |
1974年 | 9篇 |
排序方式: 共有8176条查询结果,搜索用时 15 毫秒
61.
62.
A low molecular weight fraction from chitinase digested cell walls ofT. mentagrophytes containing both polysaccharide and peptide moieties was found to have immunological reactivity at both the cellular and humoral level. This fraction (UM2(a)) was further degraded by treatment with either a combination of pronase and carboxypeptidase A or with trypsin. Peptides were separated from the carbohydrate-rich fraction by ultrafiltration. The carbohydrate-rich fraction retained the ability to induce both immediate and delayed skin reactions in sensitized guinea pigs and to stimulate the proliferation of sensitized lymphocytesin vitro. The peptide moieties retained reactivity in that they caused delayed reactions and lymphocyte proliferation but were unable to induce immediate or Arthus reactions in sensitized animals. Tryptic peptides from UM2(a) were purified by ion exchange chromatography. A high proportion of these peptides demonstrated immunological activity at both the cellular and humoral level since they were capable of inducing delayed reactions and/or lymphocyte transformation, as well as being capable of blocking the complement fixation reaction between UM2 (a) and specific antiserum.This work was supported by grant number 6411 from the Medical Research Council of Canada.A. Kh. Al-Rammahy was supported by a scholarship from the Ministry of High Education, Iraq. 相似文献
63.
64.
65.
Michael Steinwandter Birgit C. Schlick‐Steiner Gilg U. H. Seeber Florian M. Steiner Julia Seeber 《Ecology and evolution》2017,7(14):5389-5399
Although soil invertebrates play a decisive role in maintaining ecosystem functioning, little is known about their structural composition in Alpine soils and how their abundances are affected by the currently ongoing land‐use changes. In this study, we re‐assessed the soil macrofauna community structure of managed and abandoned Alpine pastureland, which has already been evaluated 14 years earlier. Our results confirm clear shifts in the community composition after abandonment, in that (1) Chilopoda and Diplopoda were recorded almost exclusively on the abandoned sites, (2) Coleoptera larvae and Diptera larvae were more abundant on the abandoned than on the managed sites, whereas (3) Lumbricidae dominated on the managed sites. By revisiting managed and abandoned sites, we infer community patterns caused by abandonment such as changes in the epigeic earthworm community structure, and we discuss seasonal and sampling effects. Our case study improves the still limited understanding of spatio‐temporal biodiversity patterns of Alpine soil communities. 相似文献
66.
67.
68.
69.
70.
Yuejia Dang Yi Wei Yanyan Wang Shaoshuai Liu Chekanova Julia Shi-Hong Zhang 《Environmental microbiology》2021,23(9):4881-4895
ATP-dependent Lon proteases function in bacterial pathogenesis by regulating the expression of the Type III secretion system; however, little is known about how Lon proteases regulate fungal pathogenesis. We previously investigated Lon-binding proteins involved in fungal pathogenesis that interact with PrePL, the smallest Magnaporthe oryzae Lon-binding protein. Here, we show that Lon cleaves PrePL and produces Pc, an extracellular 11-kDa isoform with catalase and peroxidase activity. The ΔPrePL loss-of-function strain showed stronger sporulation and accelerated disease development, suggesting a temporally specific negative regulatory mechanism controlled by PrePL in disease progression. Neither the truncated Pc, nor the full-length PrePL missing the Lon cleavage site complemented the ΔPrePL phenotype, suggesting that full-length PrePL and Pc both function in fungal development. PrePL targeted to the mitochondria undergoes hydrolysis by Lon to produce Pc, which accumulates in the fungal apoplast. Importantly, recombinant Pc induced plant defence responses and cell death after being infiltrated into selected plant leaves, indicating that it functions as an avirulence factor. This work thus reveals a novel pathogenic factor in the fungal Lon-mediated pathway. Additionally, our results provide new insight into the functions of a full-length protein and its cleaved isoform in fungal pathogenesis. 相似文献