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61.
Shusterman V Usiene I Harrigal C Lee JS Kubota T Feldman AM London B 《American journal of physiology. Heart and circulatory physiology》2002,282(6):H2076-H2083
Transgenic mice are widely used to study cardiac function, but strain-dependent differences in autonomic nervous system activity (ANSA) have not been explored. We compared 1) short-term pharmacological responses of cardiac rhythm in FVB vs. C57Black6/SV129 wild-type mice and 2) long-term physiological dynamics of cardiac rhythm and survival in tumor necrosis factor (TNF)-alpha transgenic mice with heart failure (TNF-alpha mice) on defined backgrounds. Ambulatory telemetry electrocardiographic recordings and response to saline, adrenergic, and cholinergic agents were examined in FVB and C57Black6/SV129 mice. In FVB mice, baseline heart rate (HR) was higher and did not change after injection of isoproterenol or atropine but decreased with propranolol. In C57Black6/SV129 mice, HR did not change with propranolol but increased with isoproterenol or atropine. Mean HR, but not indexes of HR variability, was an excellent predictor of response to autonomic agents. The proportion of surviving animals was higher in TNF-alpha mice on an FVB background than on a mixed FVB/C57Black6 background. The homeostatic states of ANSA are strain specific, which can explain the interstrain differences in mean HR, pharmacological responses, and survival of animals with congestive heart failure. Strain-specific differences should be considered in selecting the strains of mice used for transgenic and gene targeting experiments. 相似文献
62.
A method of mutagenic and unidirectional reassembly (MURA) that can generate libraries of DNA-shuffled and randomly truncated proteins was developed. The method involved fragmenting the template gene(s) randomly by DNase I and reassembling the small fragments with a unidirectional primer by PCR. The MURA products were treated with T4 DNA polymerase and subsequently with a restriction enzyme whose site was located on the region of the MURA primer. The N-terminal-truncated and DNA-shuffled library of a Serratia sp. phospholipase A(1) prepared by this method had an essentially random variation of truncated size and also showed point mutations associated with DNA shuffling. After high-throughput screening on triglyceride-emulsified plates, several mutants exhibiting absolute lipase activity (NPL variants) were obtained. The sequence analysis and the lipase activity assay on the NPL variants revealed that N-terminal truncations at a region beginning with amino acids 61 to 71, together with amino acid substitutions, resulted in the change of substrate specificity from a phospholipase to a lipase. We therefore suggest that the MURA method, which combines incremental truncation with DNA shuffling, can contribute to expanding the searchable sequence space in directed evolution experiments. 相似文献
63.
Netting operation to control neurofibroma of the face 总被引:3,自引:0,他引:3
When neurofibroma involves the face, it can lead to a most disfiguring, destructive, and debilitating condition. Because of the unique aesthetic and functional properties of the face, the surgeon might hesitate to remove all of the involved soft tissues and facial nerves in a radical procedure. Involvement of the craniofacial skeleton makes the treatment more difficult and complex. The treatment usually consists of excising the symptomatic lesion, and often these lesions are incompletely corrected. Regrowth after partial excision is frequent and leads to recurrence of deformity. The force of gravity plays a major role in the appearance of deformity, leading to facial tissue drooping and bulging as the mass grows and gains weight. The aim of this operation was to contain the residual mass after excision within a tight net against the force of gravity, thus limiting and preventing drooping or bulging of the facial soft tissue while preserving muscles and nerves associated with facial expression. Polytetrafluoroethylene (Teflon) mesh, used as a net, was evaluated as a replacement material for subcutaneous tissue and a substitute for superficial fascia destroyed by tumor infiltration. The mesh was suspended in the superior-posterior direction, capturing the residual mass like a net capturing fish. From 1989 to 1999, a total of eight patients underwent the netting procedure after partial excision of neurofibroma of the face. The follow-up period was 3 to 10 years. The use of Teflon mesh proved to be compatible with the surrounding tissue, endurable at follow-up, and consistent with expectations. Follow-up computed tomographic scans revealed no further visible tumor growth, and the area was contained under the mesh net with satisfactory postoperative facial contour. The advantages of this procedure are the avoidance of radical excision of facial soft tissue, preservation of remnant facial expression, and prevention of progression of facial dysmorphism. 相似文献
64.
Shin SH Shin DH Ryu PY Chung SS Rhee JH 《FEMS immunology and medical microbiology》2002,33(2):133-138
Vibrio vulnificus causes a fulminant and frequently fatal septicemia in susceptible hosts. The present study was designed to evaluate the proinflammatory cytokine profile in V. vulnificus septicemia patients' sera and the effect of doxycycline therapy on the levels of proinflammatory cytokines. Levels of proinflammatory cytokines, tumor necrosis factor (TNF)-alpha, interleukin (IL)-1beta and IL-6, were measured in the sera of V. vulnificus septicemic patients and normal healthy volunteers using colorimetric sandwich ELISA. The mean values of TNF-alpha, IL-1beta and IL-6 in the sera of V. vulnificus patients (n=33) increased by 210-, 232- and 40-fold in comparison with those of normal healthy volunteers (n=5), but only the IL-6 level showed a statistically significant difference (P<0.05) between the two groups. Sera from the cases for which doxycycline treatment histories were obvious were designated 'before-treatment' (TX). All the others were included in the after-TX group. In the before-TX group (n=5), the levels of TNF-alpha and IL-1beta significantly increased (P<0.05) in comparison with the after-TX group (n=5). IL-6 levels in the two groups showed no difference. In conclusion, the levels of the well known proinflammatory cytokines TNF-alpha, IL-1beta and IL-6 increased in the V. vulnificus septicemic patients' sera, and the levels of TNF-alpha and IL-1beta decreased significantly after doxycycline treatment. These data indicate that proinflammatory cytokines might play a critical role in V. vulnificus septicemia like in other endotoxemic shocks. The use of doxycycline as an effective bactericidal agent and as an effective modulator of proinflammatory cytokines is supported. 相似文献
65.
Yang JM Bell J Huang Y Tirado M Thomas D Forster AH Haigis RW Swanson PD Wallace RB Martinsons B Krihak M 《Biosensors & bioelectronics》2002,17(6-7):605-618
An integrated, stacked microlaboratory for performing automated electric-field-driven immunoassays and DNA hybridization assays was developed. The stacked microlaboratory was fabricated by orderly laminating several different functional layers (all 76 x 76 mm(2)) including a patterned polyimide layer with a flip-chip bonded CMOS chip, a pressure sensitive acrylic adhesive (PSA) layer with a fluidic cutout, an optically transparent polymethyl methacrylate (PMMA) film, a PSA layer with a via, a patterned polyimide layer with a flip-chip bonded silicon chip, a PSA layer with a fluidic cutout, and a glass cover plate layer. Versatility of the stacked microlaboratory was demonstrated by various automated assays. Escherichia coli bacteria and Alexa-labeled protein toxin staphylococcal enterotoxin B (SEB) were detected by electric-field-driven immunoassays on a single chip with a specific-to-nonspecific signal ratios of 4.2:1 and 3.0:1, respectively. Furthermore, by integrating the microlaboratory with a module for strand displacement amplification (SDA), the identification of the Shiga-like toxin gene (SLT1) from E. coli was accomplished within 2.5 h starting from a dielectrophoretic concentration of intact E. coli bacteria and finishing with an electric-field-driven DNA hybridization assay, detected by fluorescently labeled DNA reporter probes. The integrated microlaboratory can be potentially used in a wide range of applications including detection of bacteria and biowarfare agents, and genetic identification. 相似文献
66.
Regulation of septation and cytokinesis during resumption of cell division requires uvi31+, a UV-inducible gene of fission yeast 总被引:1,自引:0,他引:1
uvi31+ is a sequence homolog of Escherichia coli bolA gene in Schizosaccharomyces pombe, identified as a UV-inducible gene. Here, the cellular function of uvi31+ was investigated by null mutant analysis. Deletion of uvi31+ led to a delayed germination of spore and defects in subsequent cell division. However, the uvi31 mutant cell proliferated faster with smaller cell size than the wild-type cell during vegetative growth. In addition, the uvi31 mutant was sensitive to UV-light. It showed a normal cell cycle delay after UV-irradiation but displayed aberrant septum formation and defective cytokinesis when released from the UV damage checkpoint. These results suggest that uvi31+ may be involved in control of cell division, especially during the resumption from cell cycle arrest. 相似文献
67.
68.
Hematopoietic stem cells have applications in bone marrow transplantations for the treatment of hematopoietic disorders. When murine hematopoietic stem cells were cultured in 50 ml stirred bioreactors for 14 d, stem-cell-antigen-1 positive cells (hematopoietic primitive progenitor cells) and long-term culture-initiating cells (hematopoietic stem cells) grew by 5-fold and 4-fold, respectively. These results show the possibility of growing hematopoietic stem cells using a stirred bioreactor. 相似文献
69.
Human interleukin-2 (hIL-2) production in Escherichia coli and insect cell/baculovirus expression systems can be inefficient. Here we investigated secreted production of hIL-2 fused with green fluorescent protein (GFP) as a versatile fusion partner in optimized stably transfected insect Drosophila melanogaster S2 cells. This nonlytic S2 insect cell expression system employs a plasmid vector and allows for secretion of functional human proteins. We report that, following stable transfection and induction, S2 cells secreted hIL-2 as a fusion protein (approximately 2.3 microg/mL yield), with a secretion efficiency of approximately 90%. Regression analysis indicated a single linear relationship existed between GFP fluorescence and hIL-2 mass in both whole cell and secreted medium samples, indicating that in vivo monitoring and quantification of target foreign protein expression and even secretion is possible using this system. The simple comparative measurement of GFP fluorescence also allowed monitoring of secretion efficiency during periods of high GFP/hIL-2 expression. 相似文献
70.
An unstructured model was developed for batch cultivation of Corynebacterium lactofermentum (ATCC 21799) under controlled dissolved oxygen. The model is capable of predicting batch experiments performed at various initial substrate concentrations. By extending the batch culture model to a fed-batch model and using a heuristic approach to optimize the fed-batch cultivation, it is shown that fed-batch cultivation is superior to batch operation due to increased productivity at high substrate concentrations. 相似文献