Competition for sulphur in a grass-clover association

Summary A 3 × 3 factorial experiment with nitrogen and sulphur fertilizers was carried out on a grass-clover association in an area where there is little if any cyclic return of sulphur. In the absence of sulphur, grass took up 98 per cent of the total uptake of sulphur, and nitrogen fixation by associated clover was negligible. In the absence of fertilizer nitrogen, dressings of sulphur as gypsum stimulated clover growth; at a low rate of application of sulphur, nitrogen fertilizer increased grass growth and clover was suppressed. This suppression was largely overcome with a higher dressing of sulphur. Sulphur therefore must be added to the group of elements (phosphorus, nitrogen and potassium) for which grass may compete intensely when grown with clover and when nitrogen fertilizers are applied to a grass-clover association adequate supplies of sulphur must be assured in order to minimise suppression of clover. Sulphate of ammonia may stimulate clover growth under these conditions. Solubility of gypsum was low in a dry season and residual effects were pronounced.     

Intermediate sucrose octa-acetate sensitivity suggests a third allele at mouse bitter taste locus Soa and Soa-Rua identity

Mice have been characterized as either tasters or non-tastersof the bitter compound sucrose octa-acetate(SOA). However, 11of 17 supposedly non-taster inbred strains were found to avoid1 mM SOA. All 17 strains were indifferent to 0.1 mM SOA. Tasterstrains avoided both concentrations. The intermediate phenotypewas dubbed demitaster. A consistent phenotypic dominance orderwas found in crosses among both inbred and outbred strains (taster> non-taster > demitaster). Demitasters were found (withtasters) in an outbred strain showing monogenic segregationfor SOA avoidance. This, plus monogenic segregation in a back-crossof taster to demitaster inbred strains, suggested a third alleleat the Soa locus (Soa^c). Demitaster allelism was supported bythe strong associations found in 15 strains between the threeSOA phenotypes and HindIII restriction fragment patterns forthe closely linked Prp (proline rich protein) loci. SOA demitasterstrains were also intermediate in raffinose undeca-acetate (RUA)avoidance. Furthermore, B6.SW-Soa² congenic mice avoided notonly SOA, but RUA and eight other acetylated sugars. A previouslyproposed separate RUA-sensitivity gene (Rua) thus appeared tobe redundant.