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151.
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153.
Maki EC Millar JG Rodstein J Hanks LM Barbour JD 《Journal of economic entomology》2011,104(3):933-938
Larvae of Prionus californicus Motschulsky (Coleoptera: Cerambycidae) feed on the roots of many types of woody perennial crops and are serious pests of hop in the northwestern United States. The adult males are strongly attracted to a volatile sex pheromone, (3R,5S)-3,5-dimethyldodecanoic acid, that is produced by females. Here, we summarize the results of field experiments that evaluated the potential for using the synthetic pheromone (in a blend of all four possible stereoisomers) to manage infestations of P. californicus in commercial hop yards by mass trapping or mating disruption. Our research provides evidence that mass trapping may be effective in reducing mating success of the females: positioning surrogate females (sentinel traps baited with a low dose of pheromone) within a square of eight pheromone-baited traps resulted in an 88% reduction in the number of wild males that reached the sentinel traps compared with sentinel traps that were surrounded by traps baited with blank lures. Similarly, surrogate females that were surrounded by pheromone lures (without traps) were reached by 84% fewer wild males than surrogate females surrounded by blank lures, suggesting that mating disruption also may be effective. A mark-recapture experiment indicated that male P. californicus were attracted to traps baited with 1 mg of pheromone from as far away as 585 m. These studies indicate that 3,5-dimethyldodecanoic acid has very good potential for managing P. californicus in hop yards, and perhaps in other crops where it is a pest. 相似文献
154.
Marsh AK Willer DO Ambagala AP Dzamba M Chan JK Pilon R Fournier J Sandstrom P Brudno M MacDonald KS 《Journal of virology》2011,85(24):12995-13009
Cytomegalovirus (CMV) infection is the most common opportunistic infection in immunosuppressed individuals, such as transplant recipients or people living with HIV/AIDS, and congenital CMV is the leading viral cause of developmental disabilities in infants. Due to the highly species-specific nature of CMV, animal models that closely recapitulate human CMV (HCMV) are of growing importance for vaccine development. Here we present the genomic sequence of a novel nonhuman primate CMV from cynomolgus macaques (Macaca fascicularis; CyCMV). CyCMV (Ottawa strain) was isolated from the urine of a healthy, captive-bred, 4-year-old cynomolgus macaque of Philippine origin, and the viral genome was sequenced using next-generation Illumina sequencing to an average of 516-fold coverage. The CyCMV genome is 218,041 bp in length, with 49.5% G+C content and 84% protein-coding density. We have identified 262 putative open reading frames (ORFs) with an average coding length of 789 bp. The genomic organization of CyCMV is largely colinear with that of rhesus macaque CMV (RhCMV). Of the 262 CyCMV ORFs, 137 are homologous to HCMV genes, 243 are homologous to RhCMV 68.1, and 200 are homologous to RhCMV 180.92. CyCMV encodes four ORFs that are not present in RhCMV strain 68.1 or 180.92 but have homologies with HCMV (UL30, UL74A, UL126, and UL146). Similar to HCMV, CyCMV does not produce the RhCMV-specific viral homologue of cyclooxygenase-2. This newly characterized CMV may provide a novel model in which to study CMV biology and HCMV vaccine development. 相似文献
155.
Amniotes are rare in Late Carboniferous strata, especially in Europe, and despite a global diversification. They are generally more common in Lower Permian deposits. The French record around the Pennsylvanian-Cisuralian boundary is therefore re-examined. Each basin which yielded amniote remains is provided with general data on its sedimentology, stratigraphy, dating, and palaeontological content. Seven specimens are reported, of which five are synapsids and two are reptiles, each of them being a holotype. Their respective age is reassessed. The use of the regional Stephanian and Autunian stages is discussed, but their traditional sense is retained. There is currently no amniote in the Stephanian sensu stricto (= Early Gzhelian) of France. The Stephanian-Autunian Transitional Zone (= Late Gzhelian) contains the unique amniote Stereorachis blanziacensis. The Autunian sensu stricto (= Asselian) contains five, Stereorachis dominans, Haptodus baylei, Callibrachion gaudryi, Belebey augustodunensis, and Aphelosaurus lutevensis. Finally, Neosaurus cynodus can be assigned either to the Transitional Zone or to the Autunian sensu stricto. The amniote diversity increases through the Pennsylvanian-Cisuralian boundary. Despite the low number of specimens, the French record is representative of the global evolution of amniotes. 相似文献
156.
<正>出版日期:2014年5月定价:398.00元字数:180万内容简介分子生物学是一门发展迅速的学科,其知识更新之快及前沿的蓬勃发展,对人类生活产生了持久而深入的影响。Lewin的《基因》自问世以来,一 相似文献
157.
Jocelyn Charlton Richard D Williams Mark Weeks Neil J Sebire Sergey Popov Gordan Vujanic William Mifsud Marisa Alcaide-German Lee M Butcher Stephan Beck Kathy Pritchard-Jones 《Genome biology》2014,15(8)
Background
Wilms tumor is the most common pediatric renal malignancy and there is a clinical need for a molecular biomarker to assess treatment response and predict relapse. The known mutated genes in this tumor type show low mutation frequencies, whereas aberrant methylation at 11p15 is by far the most common aberration. We therefore analyzed the epigenome, rather than the genome, to identify ubiquitous tumor-specific biomarkers.Results
Methylome analysis of matched normal kidney and Wilms tumor identifies 309 preliminary methylation variable positions which we translate into three differentially methylated regions (DMRs) for use as tumor-specific biomarkers. Using two novel algorithms we show that these three DMRs are not confounded by cell type composition. We further show that these DMRs are not methylated in embryonic blastema but are intermediately methylated in Wilms tumor precursor lesions. We validate the biomarker DMRs using two independent sample sets of normal kidney and Wilms tumor and seven Wilms tumor histological subtypes, achieving 100% and 98% correct classification, respectively. As proof-of-principle for clinical utility, we successfully use biomarker DMR-2 in a pilot analysis of cell-free circulating DNA to monitor tumor response during treatment in ten patients.Conclusions
These findings define the most common methylated regions in Wilms tumor known to date which are not associated with their embryonic origin or precursor stage. We show that this tumor-specific methylated DNA is released into the blood circulation where it can be detected non-invasively showing potential for clinical utility.Electronic supplementary material
The online version of this article (doi:10.1186/s13059-014-0434-y) contains supplementary material, which is available to authorized users. 相似文献158.
Kenneth Soyemi Andrew Medina-Marino Ronda Sinkowitz-Cochran Amy Schneider Rashid Njai Marian McDonald Maleeka Glover Jocelyn Garcia Allison E. Aiello 《PloS one》2014,9(4)
During late April 2009, the first cases of 2009 pandemic influenza A (H1N1) (pH1N1) in Illinois were reported. On-going, sustained local transmission resulted in an estimated 500,000 infected persons. We conducted a mixed method analysis using both quantitative (surveillance) and qualitative (interview) data; surveillance data was used to analyze demographic distribution of hospitalized cases and follow-up interview data was used to assess health seeking behavior. Invitations to participate in a telephone interview were sent to 120 randomly selected Illinois residents that were hospitalized during April–December 2009. During April–December 2009, 2,824 pH1N1 hospitalizations occurred in Illinois hospitals; median age (interquartile range) at admission was 24 (range: 6–49) years. Hospitalization rates/100,000 persons for blacks and Hispanics, regardless of age or sex were 2–3 times greater than for whites (blacks, 36/100,000 (95% Confidence Interval ([95% CI], 33–39)); Hispanics, 35/100,000 [95%CI,32–37] (; whites, 13/100,000[95%CI, 12–14); p<0.001). Mortality rates were higher for blacks (0.9/100,000; p<0.09) and Hispanics (1/100,000; p<0.04) when compared with the mortality rates for whites (0.6/100,000). Of 33 interview respondents, 31 (94%) stated that they had heard of pH1N1 before being hospitalized, and 24 (73%) did not believed they were at risk for pH1N1. On average, respondents reported experiencing symptoms for 2 days (range: 1–7) before seeking medical care. When asked how to prevent pH1N1 infection in the future, the most common responses were getting vaccinated and practicing hand hygiene. Blacks and Hispanics in Illinois experienced disproportionate pH1N1 hospitalization and mortality rates. Public health education and outreach efforts in preparation for future influenza pandemics should include prevention messaging focused on perception of risk, and ensure community wide access to prevention messages and practices. 相似文献
159.
Isabelle M. C?té Emily S. Darling Luis Malpica-Cruz Nicola S. Smith Stephanie J. Green Jocelyn Curtis-Quick Craig Layman 《PloS one》2014,9(4)
As a result of being hunted, animals often alter their behaviour in ways that make future encounters with predators less likely. When hunting is carried out for conservation, for example to control invasive species, these behavioural changes can inadvertently impede the success of future efforts. We examined the effects of repeated culling by spearing on the behaviour of invasive predatory lionfish (Pterois volitans/miles) on Bahamian coral reef patches. We compared the extent of concealment and activity levels of lionfish at dawn and midday on 16 coral reef patches off Eleuthera, The Bahamas. Eight of the patches had been subjected to regular daytime removals of lionfish by spearing for two years. We also estimated the distance at which lionfish became alert to slowly approaching divers on culled and unculled reef patches. Lionfish on culled reefs were less active and hid deeper within the reef during the day than lionfish on patches where no culling had occurred. There were no differences at dawn when removals do not take place. Lionfish on culled reefs also adopted an alert posture at a greater distance from divers than lionfish on unculled reefs. More crepuscular activity likely leads to greater encounter rates by lionfish with more native fish species because the abundance of reef fish outside of shelters typically peaks at dawn and dusk. Hiding deeper within the reef could also make remaining lionfish less likely to be encountered and more difficult to catch by spearfishers during culling efforts. Shifts in the behaviour of hunted invasive animals might be common and they have implications both for the impact of invasive species and for the design and success of invasive control programs. 相似文献
160.
Steven A. Safren Katie B. Biello Laura Smeaton Matthew J. Mimiaga Ann Walawander Javier R. Lama Aadia Rana Mulinda Nyirenda Virginia M. Kayoyo Wadzanai Samaneka Anjali Joglekar David Celentano Ana Martinez Jocelyn E. Remmert Aspara Nair Umesh G. Lalloo Nagalingeswaran Kumarasamy James Hakim Thomas B. Campbell for the PEARLS Study Team 《PloS one》2014,9(8)