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11.
The Tomato Sequencing Project, the first cornerstone of the International Solanaceae Project (SOL) 总被引:4,自引:0,他引:4
Mueller LA Tanksley SD Giovannoni JJ van Eck J Stack S Choi D Kim BD Chen M Cheng Z Li C Ling H Xue Y Seymour G Bishop G Bryan G Sharma R Khurana J Tyagi A Chattopadhyay D Singh NK Stiekema W Lindhout P Jesse T Lankhorst RK Bouzayen M Shibata D Tabata S Granell A Botella MA Giuliano G Frusciante L Causse M Zamir D 《Comparative and Functional Genomics》2005,6(3):153-158
The genome of tomato (Solanum lycopersicum) is being sequenced by an international
consortium of 10 countries (Korea, China, the United Kingdom, India, The
Netherlands, France, Japan, Spain, Italy and the United States) as part of a larger initiative
called the ‘International Solanaceae Genome Project (SOL): Systems Approach
to Diversity and Adaptation’. The goal of this grassroots initiative, launched in
November 2003, is to establish a network of information, resources and scientists
to ultimately tackle two of the most significant questions in plant biology and agriculture:
(1) How can a common set of genes/proteins give rise to a wide range of
morphologically and ecologically distinct organisms that occupy our planet? (2) How
can a deeper understanding of the genetic basis of plant diversity be harnessed to
better meet the needs of society in an environmentally friendly and sustainable manner?
The Solanaceae and closely related species such as coffee, which are included
in the scope of the SOL project, are ideally suited to address both of these questions.
The first step of the SOL project is to use an ordered BAC approach to generate a
high quality sequence for the euchromatic portions of the tomato as a reference for
the Solanaceae. Due to the high level of macro and micro-synteny in the Solanaceae
the BAC-by-BAC tomato sequence will form the framework for shotgun sequencing
of other species. The starting point for sequencing the genome is BACs anchored
to the genetic map by overgo hybridization and AFLP technology. The overgos are
derived from approximately 1500 markers from the tomato high density F2-2000
genetic map (http://sgn.cornell.edu/). These seed BACs will be used as anchors from
which to radiate the tiling path using BAC end sequence data. Annotation will be
performed according to SOL project guidelines. All the information generated under
the SOL umbrella will be made available in a comprehensive website. The information
will be interlinked with the ultimate goal that the comparative biology of the
Solanaceae—and beyond—achieves a context that will facilitate a systems biology
approach. 相似文献
12.
Cell line development is a critical step in the establishment of a biopharmaceutical manufacturing process. Current protocols rely on random transgene integration and amplification. Due to considerable variability in transgene integration profiles, this workflow results in laborious screening campaigns before stable producers can be identified. Alternative approaches for transgene dosage increase and integration are therefore highly desirable. In this study, we present a novel strategy for the rapid design, construction, and genomic integration of engineered multiple-copy gene constructs consisting of up to 10 gene expression cassettes. Key to this strategy is the diversification, at the sequence level, of the individual gene cassettes without altering their protein products. We show a computational workflow for coding and regulatory sequence diversification and optimization followed by experimental assembly of up to nine gene copies and a sentinel reporter on a contiguous scaffold. Transient transfections in CHO cells indicates that protein expression increases with the gene copy number on the scaffold. Further, we stably integrate these cassettes into a pre-validated genomic locus. Altogether, our findings point to the feasibility of engineering a fully mapped multi-copy recombinant protein ‘production island’ in a mammalian cell line with greatly reduced screening effort, improved stability, and predictable product titers. 相似文献
13.
Yoshihisa ABE Mantu BHUYAN Jiten MECH Pranab R. BHATTACHARYYA Tatsuya IDE Misako MISHIMA Chika SUYAMA Shinsuke SATO Kazunori MATSUO Nakatada WACHI 《Entomological Science》2012,15(3):340-342
We report for the first time the occurrence of an oak gall wasp Andricus mukaigawae (Mukaigawa) (Hymenoptera: Cynipidae) on the deciduous oak Quercus griffithii Hook. F & Thomson ex Miq. in India. Andricus mukaigawae is the only cynipid species that has been observed to induce galls on deciduous oak species in India to date. In addition, this is the first record of a gall wasp species with a distribution extending all the way from the eastern Palearctic region to the Indian subcontinent, suggesting the existence of a close relationship between cynipid faunas on deciduous oak trees in the two regions. 相似文献
14.
L. D. MECH 《The Journal of wildlife management》2007,71(3):920-923
Abstract: I present marrow fat (MF) data from a large sample of white-tailed deer fawns killed by wolves and a sample of fawns that died by accident in a single area, and I use these data to explore the extent that poor nutritional condition may have predisposed fawns to wolf predation. Percent MF of 110 5-10-month-old white-tailed deer (Odocoileus virginianus) fawns killed by wolves (Canis lupus) from November through April 1984-2002 in northeastern Minnesota, USA, was lower than MF for 23 fawns killed by accidents in the same area and period. The MF of both male and female wolf-killed fawns decreased over winter. The MF of male fawns decreased as a snow-depth index increased, but MF of females showed little relationship to the snow-depth index and was higher than that of males. Poor nutritional condition is one factor that predisposes deer fawns to wolf predation during winter and spring. This information expands our knowledge of wolf-prey relations by documenting that, even with younger prey animals that might be thought vulnerable because of youth alone, poor nutritional condition also is an important factor predisposing them to wolf predation. 相似文献
15.
DOMINIC J. DEMMA SHANNON M. BARBER-MEYER L. DAVID MECH 《The Journal of wildlife management》2007,71(8):2767-2775
ABSTRACT We conducted a pilot study to test the usefulness of Global Positioning System (GPS) collars for investigating wolf (Canis lupus) predation on white-tailed deer (Odocoileus virginianus) fawns. Using GPS collars with short location-attempt intervals on 5 wolves and 5 deer during summers 2002–2004 in northeastern Minnesota, USA, demonstrated how this approach could provide new insights into wolf hunting behavior of fawns. For example, a wolf traveled ≥1.5–3.0 km and spent 20–22 hours in the immediate vicinity of known fawn kill sites and ≥0.7 km and 8.3 hours at scavenging sites. Wolf travel paths indicated that wolves intentionally traveled into deer summer ranges, traveled ≥0.7–4.2 km in such ranges, and spent <1–22 hours per visit. Each pair of 3 GPS-collared wolf pack members were located together for ≤6% of potential locations. From GPS collar data, we estimated that each deer summer range in a pack territory containing 5 wolves ≥1 year old and hunting individually would be visited by a wolf on average every 3–5 days. This approach holds great potential for investigating summer hunting behavior of wolves in areas where direct observation is impractical or impossible. 相似文献
16.
L. DAVID MECH 《The Journal of wildlife management》2010,74(7):1421-1424
Abstract: As gray wolves (Canis lupus) are removed from the federal Endangered Species List, management reverts to the states. Eventually most states will probably allow public wolf harvesting. Open seasons between about 1 November and 1 March accord more with basic wolf biology than during other times. Managers who consider wolf biology and public sensitivities, adapt public-taking regulations accordingly, and adjust harvest regulations as they learn will be best able to maximize the recreational value of wolf harvesting, minimize public animosity toward it, and meet their harvest objectives. 相似文献
17.
Molecular Biology Reports - Acacetin, a bioflavanoid, contains anti-inflammatory and anti-cancer activities as shown in different experimental models. However, its anticancer potential and... 相似文献
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