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排序方式: 共有475条查询结果,搜索用时 15 毫秒
121.
Lee M. Kaplan John A. Fallon Edward C. Mun Alan M. Harvey William V. Kastrinakis Elvira Q. Johnson Robert S. Nierman Christopher R. Keroack 《Obesity (Silver Spring, Md.)》2005,13(2):290-300
Objective: To review the use and usefulness of billing codes for services related to weight loss surgery (WLS) and to examine third party reimbursement policies for these services. Research Methods and Procedures: The Task Group carried out a systematic search of MEDLINE, the Internet, and the trade press for publications on WLS, coding, reimbursement, and coding and reimbursement policy. Twenty‐eight articles were each reviewed and graded using a system based on established evidence‐based models. The Massachusetts Dietetics Association provided reimbursement data for nutrition services. Three suppliers of laparoscopic WLS equipment provided summaries of coding and reimbursement information. WLS program directors were surveyed for information on use of procedure codes related to WLS. Results: Recommendations focused on correcting or improving on the current lack of congruity among coding practices, reimbursement policies, and accepted clinical practice; lack of uniform coding and reimbursement data across institutions; inconsistent and/or inaccurate diagnostic and billing codes; inconsistent insurance reimbursement criteria; and inability to leverage reimbursement and coding data to track outcomes, identify best practices, and perform accurate risk‐benefit analyses. Discussion: Rapid changes in the prevalence of obesity, our understanding of its clinical impact, and the technologies for surgical treatment have yet to be adequately reflected in coding, coverage, and reimbursement policies. Issues identified as key to effective change include improved characterization of the risks, benefits, and costs of WLS; anticipation and monitoring of technological advances; encouragement of consistent patterns of insurance coverage; and promotion of billing codes for WLS procedures that facilitate accurate tracking of clinical use and outcomes. 相似文献
122.
Daniel Asarnow Bei Wang Wen-Hsin Lee Yuanyu Hu Ching-Wen Huang Bryan Faust Patricia Miang Lon Ng Eve Zi Xian Ngoh Markus Bohn David Bulkley Andrés Pizzorno Beatrice Ary Hwee Ching Tan Chia Yin Lee Rabiatul Adawiyah Minhat Olivier Terrier Mun Kuen Soh Frannie Jiuyi Teo Cheng-I Wang 《Cell》2021,184(12):3192-3204.e16
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124.
Dispersal distances of 17 species of butterflies in tropical Singapore were significantly greater in forest than in urban habitat. Butterflies in urban plots frequently moved within suitable habitat (park/grassland) patches but rarely crossed non-habitat patches suggesting potential isolation and a need for urban corridors. 相似文献
125.
Soyoun Kim Sang Mun Bae Junyoung Seo Kiweon Cha Meilan Piao Sun-Ji Kim Hye-Nam Son Rang-Woon Park Byung-Heon Lee In-San Kim 《PloS one》2015,10(3)
A number of peptide-based indicators have been identified and reported as potential apoptosis probes, offering great promise for early assessment of therapeutic efficacy in several types of cancer. Direct comparison of the newly developed probes with previously used ones would be an important step in assessing possible applications. Here, we compared the newly identified peptide-based phosphatidylserine (PS) indicator PSP1 (CLSYYPSYC) with annexin V, a common probe for molecular imaging of apoptotic cells, with respect to PS binding kinetics, apoptotic cell-targeting ability, and the efficacy of homing to apoptotic tumor cells in a mouse model after treatment with the anticancer agent camptothecin. Our results indicate that PSP1 efficiently targeted apoptotic cells and generated apoptosis/tumor-specific signals after cancer treatment in the animal model, whereas a similar dose of annexin V showed weak signals. The formation of a stable complex of PSP1 with PS might be one reason for the efficient in vivo targeting. We suggest that PSP1 has potential advantages for in vivo apoptotic cell imaging and could serve as a platform for the development of de novo peptide-based probes for apoptosis. 相似文献
126.
Peck Shen Mun Hua Nong Ting Teng Aik Ong Chew Ming Wong Kwan Hong Ng Yip Boon Chong 《PloS one》2015,10(6)
This paper investigates the dielectric properties of urine in normal subjects and subjects with chronic kidney disease (CKD) at microwave frequency of between 0.2 GHz and 50 GHz. The measurements were conducted using an open-ended coaxial probe at room temperature (25°C), at 30°C and at human body temperature (37°C). There were statistically significant differences in the dielectric properties of the CKD subjects compared to those of the normal subjects. Statistically significant differences in dielectric properties were observed across the temperatures for normal subjects and CKD subjects. Pearson correlation test showed the significant correlation between proteinuria and dielectric properties. The experimental data closely matched the single-pole Debye model. The relaxation dispersion and relaxation time increased with the proteinuria level, while decreasing with the temperature. As for static conductivity, it increased with proteinuria level and temperature. 相似文献
127.
Algal chloroplast produced camelid VHH antitoxins are capable of neutralizing botulinum neurotoxin 下载免费PDF全文
Daniel J. Barrera Julian N. Rosenberg Joanna G. Chiu Yung‐Nien Chang Michelle Debatis Soo‐Mun Ngoi John T. Chang Charles B. Shoemaker George A. Oyler Stephen P. Mayfield 《Plant biotechnology journal》2015,13(1):117-124
We have produced three antitoxins consisting of the variable domains of camelid heavy chain‐only antibodies (VHH) by expressing the genes in the chloroplast of green algae. These antitoxins accumulate as soluble proteins capable of binding and neutralizing botulinum neurotoxin. Furthermore, they accumulate at up to 5% total soluble protein, sufficient expression to easily produce these antitoxins at scale from algae. The genes for the three different antitoxins were transformed into Chlamydomonas reinhardtii chloroplasts and their products purified from algae lysates and assayed for in vitro biological activity using toxin protection assays. The produced antibody domains bind to botulinum neurotoxin serotype A (BoNT/A) with similar affinities as camelid antibodies produced in Escherichia coli, and they are similarly able to protect primary rat neurons from intoxication by BoNT/A. Furthermore, the camelid antibodies were produced in algae without the use of solubilization tags commonly employed in E. coli. These camelid antibody domains are potent antigen‐binding proteins and the heterodimer fusion protein containing two VHH domains was capable of neutralizing BoNT/A at near equimolar concentrations with the toxin. Intact antibody domains were detected in the gastrointestinal (GI) tract of mice treated orally with antitoxin‐producing microalgae. These findings support the use of orally delivered antitoxins produced in green algae as a novel treatment for botulism. 相似文献
128.
Adeline S. L. Yeo Nur Atiqah Azhar Wanyi Yeow C. Conover Talbot Jr Mohammad Asif Khan Esaki M. Shankar Anusyah Rathakrishnan Azliyati Azizan Seok Mui Wang Siew Kim Lee Mun Yik Fong Rishya Manikam Shamala Devi Sekaran 《PloS one》2014,9(4)
Objectives
Dengue represents one of the most serious life-threatening vector-borne infectious diseases that afflicts approximately 50 million people across the globe annually. Whilst symptomatic infections are frequently reported, asymptomatic dengue remains largely unnoticed. Therefore, we sought to investigate the immune correlates conferring protection to individuals that remain clinically asymptomatic.Methods
We determined the levels of neutralizing antibodies (nAbs) and gene expression profiles of host immune factors in individuals with asymptomatic infections, and whose cognate household members showed symptoms consistent to clinical dengue infection.Results
We observed broad down-regulation of host defense response (innate, adaptive and matrix metalloprotease) genes in asymptomatic individuals as against symptomatic patients, with selective up-regulation of distinct genes that have been associated with protection. Selected down-regulated genes include: TNF α (TNF), IL8, C1S, factor B (CFB), IL2, IL3, IL4, IL5, IL8, IL9, IL10 and IL13, CD80, CD28, and IL18, MMP8, MMP10, MMP12, MMP15, MMP16, and MMP24. Selected up-regulated genes include: RANTES (CCL5), MIP-1α (CCL3L1/CCL3L3), MIP-1β (CCL4L1), TGFβ (TGFB), and TIMP1.Conclusion
Our findings highlight the potential association of certain host genes conferring protection against clinical dengue. These data are valuable to better explore the mysteries behind the hitherto poorly understood immunopathogenesis of subclinical dengue infection. 相似文献129.
Longitudinal Configural Frequency Analysis (CFA) seeks to identify, at the manifest variable level, those temporal patterns that are observed more frequently (CFA types) or less frequently (CFA antitypes) than expected with reference to a base model. This article discusses, compares, and extends two base models of interest in longitudinal data analysis. The first of these, Prediction CFA (P-CFA), is a base model that can be used in the configural analysis of both cross-sectional and longitudinal data. This model takes the associations among predictors and among criteria into account. The second base model, Auto-Association CFA (A-CFA), was specifically designed for longitudinal data. This model takes the auto-associations among repeatedly observed variables into account. Both models are extended to accommodate covariates, for example, stratification variables. Application examples are given using data from a longitudinal study of domestic violence. It is illustrated that CFA is able to yield results that are not redundant with results from log-linear modeling or multinomial regression. It is concluded that CFA is particularly useful in the context of person-oriented research. 相似文献
130.
Chang S Wang RH Akagi K Kim KA Martin BK Cavallone L;Kathleen Cuningham Foundation Consortium for Research into Familial Breast Cancer 《Nature medicine》2011,17(10):1275-1282
BRCA1, a well-known tumor suppressor with multiple interacting partners, is predicted to have diverse biological functions. However, so far its only well-established role is in the repair of damaged DNA and cell cycle regulation. In this regard, the etiopathological study of low-penetrant variants of BRCA1 provides an opportunity to uncover its other physiologically important functions. Using this rationale, we studied the R1699Q variant of BRCA1, a potentially moderate-risk variant, and found that it does not impair DNA damage repair but abrogates the repression of microRNA-155 (miR-155), a bona fide oncomir. Mechanistically, we found that BRCA1 epigenetically represses miR-155 expression via its association with HDAC2, which deacetylates histones H2A and H3 on the miR-155 promoter. We show that overexpression of miR-155 accelerates but the knockdown of miR-155 attenuates the growth of tumor cell lines in vivo. Our findings demonstrate a new mode of tumor suppression by BRCA1 and suggest that miR-155 is a potential therapeutic target for BRCA1-deficient tumors. 相似文献