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11.
Magni Olsen Kyrkjeeide Kristian Hassel Kjell Ivar Flatberg A. Jonathan Shaw Narjes Yousefi Hans K. Sten?ien 《PloS one》2016,11(2)
Spore-producing organisms have small dispersal units enabling them to become widespread across continents. However, barriers to gene flow and cryptic speciation may exist. The common, haploid peatmoss Sphagnum magellanicum occurs in both the Northern and Southern hemisphere, and is commonly used as a model in studies of peatland ecology and peatmoss physiology. Even though it will likely act as a rich source in functional genomics studies in years to come, surprisingly little is known about levels of genetic variability and structuring in this species. Here, we assess for the first time how genetic variation in S. magellanicum is spatially structured across its full distribution range (Northern Hemisphere and South America). The morphologically similar species S. alaskense was included for comparison. In total, 195 plants were genotyped at 15 microsatellite loci. Sequences from two plastid loci (trnG and trnL) were obtained from 30 samples. Our results show that S. alaskense and almost all plants of S. magellanicum in the northern Pacific area are diploids and share the same gene pool. Haploid plants occur in South America, Europe, eastern North America, western North America, and southern Asia, and five genetically differentiated groups with different distribution ranges were found. Our results indicate that S. magellanicum consists of several distinct genetic groups, seemingly with little or no gene flow among them. Noteworthy, the geographical separation of diploids and haploids is strikingly similar to patterns found within other haploid Sphagnum species spanning the Northern Hemisphere. Our results confirm a genetic division between the Beringian and the Atlantic that seems to be a general pattern in Sphagnum taxa. The pattern of strong genetic population structuring throughout the distribution range of morphologically similar plants need to be considered in future functional genomic studies of S. magellanicum. 相似文献
12.
Tiffany M. Tsang Jeffrey S. Wiese Suleyman Felek Malte Kronshage Eric S. Krukonis 《PloS one》2013,8(12)
The Yersinia pestis adhesin Ail mediates host cell binding and facilitates delivery of cytotoxic Yop proteins. Ail from Y. pestis and Y. pseudotuberculosis is identical except for one or two amino acids at positions 43 and 126 depending on the Y. pseudotuberculosis strain. Ail from Y. pseudotuberculosis strain YPIII has been reported to lack host cell binding ability, thus we sought to determine which amino acid difference(s) are responsible for the difference in cell adhesion. Y. pseudotuberculosis YPIII Ail expressed in Escherichia coli bound host cells, albeit at ∼50% the capacity of Y. pestis Ail. Y. pestis Ail single mutants, Ail-E43D and Ail-F126V, both have decreased adhesion and invasion in E. coli when compared to wild-type Y. pestis Ail. Y. pseudotuberculosis YPIII Ail also had decreased binding to the Ail substrate fibronectin, relative to Y. pestis Ail in E. coli. When expressed in Y. pestis, there was a 30–50% decrease in adhesion and invasion depending on the substitution. Ail-mediated Yop delivery by both Y. pestis Ail and Y. pseudotuberculosis Ail were similar when expressed in Y. pestis, with only Ail-F126V giving a statistically significant reduction in Yop delivery of 25%. In contrast to results in E. coli and Y. pestis, expression of Ail in Y. pseudotuberculosis led to no measurable adhesion or invasion, suggesting the longer LPS of Y. pseudotuberculosis interferes with Ail cell-binding activity. Thus, host context affects the binding activities of Ail and both Y. pestis and Y. pseudotuberculosis Ail can mediate cell binding, cell invasion and facilitate Yop delivery. 相似文献
13.
Application of a bioinformatics training delivery method for reaching dispersed and distant trainees
Christina R. Hall Philippa C. Griffin Andrew J. Lonie Jeffrey H. Christiansen 《PLoS computational biology》2021,17(3)
Many initiatives have addressed the global need to upskill biologists in bioinformatics tools and techniques. Australia is not unique in its requirement for such training, but due to its large size and relatively small and geographically dispersed population, Australia faces specific challenges. A combined training approach was implemented by the authors to overcome these challenges. The “hybrid” method combines guidance from experienced trainers with the benefits of both webinar-style delivery and concurrent face-to-face hands-on practical exercises in classrooms. Since 2017, the hybrid method has been used to conduct 9 hands-on bioinformatics training sessions at international scale in which over 800 researchers have been trained in diverse topics on a range of software platforms. The method has become a key tool to ensure scalable and more equitable delivery of short-course bioinformatics training across Australia and can be easily adapted to other locations, topics, or settings. 相似文献
14.
Jeffrey Pomerance Richard Johnson Shoba Kagal Philip Brooks Malcolm Margolin Arthur Allen 《The Western journal of medicine》1980,133(4):289-291
Recently, there has been increased interest in the influence of maternal prenatal nutrition on the course and outcome of pregnancy. Evidence has accumulated that a woman''s weight before pregnancy and the weight gained during pregnancy directly affect infant birth weight, incidence of neonatal mortality, and growth and development of the infant during the first year of life. Although recent recommendations for weight gain in pregnancy have been liberalized, a survey of 195 pregnant women who had prenatal visits in both clinic and private offices showed deficiencies in their understanding of the subject. Some 37 percent of women believed they should gain 20 pounds (9 kg) or less during pregnancy. Eight percent admitted to dieting before at least one antenatal visit and 54 percent thought their doctor would not be concerned about too little weight gained during pregnancy. This suggests that many women and some doctors are still ignorant of current concepts of proper nutrition during pregnancy. Apparently, increased lay and professional educational efforts are needed. 相似文献
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17.
Transnational Migration in Rural Oaxaca, Mexico: Dependency, Development, and the Household 总被引:1,自引:0,他引:1
Contradictory models of dependency and development have dominated the discussion of migration between Mexico and the United States. Transnational models of migration resolve these contradictions by defining a series of interdependencies (economy and society, for example). Using data collected in a rural Zapotec community in Oaxaca, Mexico, this article focuses on three areas: the stage-specific development of transnational movement; the domestic cycle, household decision making, and migration/remittance outcomes; and the changing nature of community participation. Rooting the discussion in household decision making captures the important role local social variability and economic dynamism play in understanding transnational processes and advancing migration studies. [ households, migration, transnationalism, dependency and development, Oaxaca, Mexico ] 相似文献
18.
Food sharing: a model of manipulation by harassment 总被引:3,自引:1,他引:2
Most analyses of food-sharing behavior invoke complex explanationssuch as indirect and delayed benefits for sharing via kin selectionand reciprocal altruism. However, food sharing can be a moregeneral phenomenon accounted for by more parsimonious, mutualisticexplanations. We propose a game theoretical model of a generalsharing situation in which food owners share because it is
in their own self-interestthey avoid high costs associatedwith beggar harassment. When beggars harass, owners may benefitfrom sharing part of the food if their consumption rate islow relative to the rate of cost accrual. Our model predictsthat harassment can be a profitable strategy for beggars if
they reap some direct benefits from harassing other than sharedfood (such as picking up scraps). Therefore, beggars may manipulatethe owner's fitness payoffs in such a way as to make sharingmutualistic. 相似文献
19.
Edmund H. Frank Boyce W. Burge Boleslaw H. Liwnicz Linda J. Lotspeich Jocelyn C. White Steven L. Wechsler Frank H. Mayfield Jeffrey T. Keller 《Experimental cell research》1983,146(2):371-376
Cells from cranial and spinal arachnoid membranes of humans were grown in culture. Their growth characteristics, morphology and details of their cytoskeletal composition are described. Arachnoid membranes, obtained at autopsy, were finely minced and incubated in tissue culture medium. Monolayers of cells of homogeneous morphology grew from these tissue fragments. The cells were flat and polygonal. They divided slowly to form non-overlapping monolayers of low cell density. Electron microscopic examination of cultured arachnoid cells revealed numerous desmosome-like tight junctions and abundant intermediate filaments (tonofilaments). Both morphological features are characteristic of arachnoid cells in situ, but not of cells in the fibroblast-rich dura mater. Immunofluorescence microscopy with monoclonal antibodies demonstrated cytokeratin in the cytoplasm of primary cultures of arachnoid cells. Thus we demonstrated that these cultured cells retained certain of the specific differentiated properties of arachnoid cells in situ and that they are not fibroblasts (which lack tight junctions and cytokeratins). To our knowledge, there have been no previous reports of in vitro growth of arachnoid cells. This in vitro model should be useful in studying the response of arachnoid cells to a variety of substances thought to be involved in the chronic inflammatory condition of the meninges known as arachnoiditis. 相似文献
20.
Jeffrey P Mower Pascal Touzet Julie S Gummow Lynda F Delph Jeffrey D Palmer 《BMC evolutionary biology》2007,7(1):135