全文获取类型
收费全文 | 614篇 |
免费 | 29篇 |
出版年
2024年 | 2篇 |
2023年 | 8篇 |
2022年 | 2篇 |
2021年 | 23篇 |
2020年 | 5篇 |
2019年 | 4篇 |
2018年 | 7篇 |
2017年 | 12篇 |
2016年 | 14篇 |
2015年 | 36篇 |
2014年 | 38篇 |
2013年 | 45篇 |
2012年 | 57篇 |
2011年 | 46篇 |
2010年 | 39篇 |
2009年 | 26篇 |
2008年 | 32篇 |
2007年 | 35篇 |
2006年 | 29篇 |
2005年 | 30篇 |
2004年 | 24篇 |
2003年 | 19篇 |
2002年 | 27篇 |
2001年 | 9篇 |
2000年 | 5篇 |
1999年 | 3篇 |
1998年 | 4篇 |
1997年 | 6篇 |
1996年 | 3篇 |
1995年 | 3篇 |
1993年 | 3篇 |
1992年 | 5篇 |
1991年 | 4篇 |
1990年 | 2篇 |
1989年 | 6篇 |
1988年 | 2篇 |
1987年 | 2篇 |
1986年 | 3篇 |
1985年 | 1篇 |
1984年 | 1篇 |
1983年 | 1篇 |
1982年 | 6篇 |
1981年 | 1篇 |
1979年 | 5篇 |
1978年 | 1篇 |
1976年 | 3篇 |
1975年 | 1篇 |
1974年 | 1篇 |
1972年 | 1篇 |
1962年 | 1篇 |
排序方式: 共有643条查询结果,搜索用时 272 毫秒
41.
The preferential use of one hand over the other is considered the primary behavioral expression of structural and functional asymmetry in cerebral structures, which is a decisive factor in human evolution. We present the first analysis of manual laterality in a form of object play—stone handling (SH) behavior—in a free-ranging group of Japanese macaques. Defined as a stone-directed manipulative activity, and comprised of multiple one-handed SH patterns (e.g., grabbing a stone in one hand and cradling it against its chest), as well as coordinated two-handed SH patterns with manual role differentiation (e.g., holding a stone with one hand and rubbing it with the other), SH behavior is a good candidate for the study of hand lateralization. We systematically followed the methodological framework developed by McGrew and Marchant (1997) to measure and analyze the presence, strength, and direction of manual preference in the performance of SH behavior and in various SH patterns, both at the individual and group level. Some individuals showed a significant manual lateral bias on a single SH pattern (hand preference), whereas others showed consistency in laterality across all or most of the SH patterns they performed (hand specialization). At the group level, we found that, although their collective distribution of left versus right remained random, most subjects were either significantly but incompletely lateralized, or completely lateralized within particular SH patterns (pattern specialization), but not across all SH patterns (no handedness for SH behavior as a whole). As predicted by the task-complexity model, hand specialization and handedness were stronger in the coordinated bimanual SH patterns than in the unimanual patterns. We discuss the implications of our findings for the evolution of manual preferences in noninstrumental object manipulation versus stone tool use in nonhuman primates and hominins. 相似文献
42.
Peter J. Fashing Felix Mulindahabi Jean-Baptiste Gakima Michel Masozera Ian Mununura Andrew J. Plumptre Nga Nguyen 《International journal of primatology》2007,28(3):529-550
With group sizes sometimes >300 individuals, the Angolan black-and-white colobus (Colobus angolensis ruwenzorii) population
in Nyungwe Forest, Rwanda is an intriguing exception to the tendency for folivores to live in smaller groups than expected
relative to body size. Researchers have hypothesized that the unusually high quality of foliage at Nyungwe allows colobus
there to avoid intragroup feeding competition, releasing constraints on the formation of large groups (Fimbel et al., 2001). We collected data on the activity and ranging patterns of a >300-member Nyungwe colobus group and compared our results
to those from smaller groups in other black-and-white colobus (Colobus spp.) populations. Colobus at Nyungwe spent far more
time feeding and moving (62%) and far less time resting (32%) than black-and-white colobus at any other site. The annual home
range of the Nyungwe colobus was also many times larger (95% minimum convex polygon: 20.7 km
2
; 95% fixed kernel: 24.4 km
2
) than those for other populations. We terminated our research after the group engaged in an unprecedented migration among
black-and-white colobus by moving 13 km south of their former range. Our results suggest that intragroup scramble competition
may be more intense than originally believed within the large colobus groups at Nyungwe and that long periods of resource
renewal may be necessary after a large colobus group passes through an area, thereby potentially helping to explain their
wide ranging patterns. We discuss the socioecological convergence between the Nyungwe colobus and Chinese snub-nosed monkeys
(Rhinopithecus spp.) and suggest directions for future research on the unique black-and-white colobus population at Nyungwe.
相似文献
Peter J. FashingEmail: |
43.
Julier B Huguet T Chardon F Ayadi R Pierre JB Prosperi JM Barre P Huyghe C 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2007,114(8):1391-1406
In many legume crops, especially in forage legumes, aerial morphogenesis defined as growth and development of plant organs,
is an essential trait as it determines plant and seed biomass as well as forage quality (protein concentration, dry matter
digestibility). Medicago truncatula is a model species for legume crops. A set of 29 accessions of M. truncatula was evaluated for aerial morphogenetic traits. A recombinant inbred lines (RILs) mapping population was used for analysing
quantitative variation in aerial morphogenetic traits and QTL detection. Genes described to be involved in aerial morphogenetic
traits in other species were mapped to analyse co-location between QTLs and genes. A large variation was found for flowering
date, morphology and dynamics of branch elongation among the 29 accessions and within the RILs population. Flowering date
was negatively correlated to main stem and branch length. QTLs were detected for all traits, and each QTL explained from 5.2
to 59.2% of the phenotypic variation. A QTL explaining a large part of genetic variation for flowering date and branch growth
was found on chromosome 7. The other chromosomes were also involved in the variation detected in several traits. Mapping of
candidate genes indicates a co-location between a homologue of Constans gene or a flowering locus T (FT) gene and the QTL
of flowering date on chromosome 7. Other candidate genes for several QTLs are described.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献
44.
Garlatti V Martin L Gout E Reiser JB Fujita T Arlaud GJ Thielens NM Gaboriaud C 《The Journal of biological chemistry》2007,282(49):35814-35820
Ficolins are soluble oligomeric proteins with lectin-like activity, assembled from collagen fibers prolonged by fibrinogen-like recognition domains. They act as innate immune sensors by recognizing conserved molecular markers exposed on microbial surfaces and thereby triggering effector mechanisms such as enhanced phagocytosis and inflammation. In humans, L- and H-ficolins have been characterized in plasma, whereas a third species, M-ficolin, is secreted by monocytes and macrophages. To decipher the molecular mechanisms underlying their recognition properties, we previously solved the structures of the recognition domains of L- and H-ficolins, in complex with various model ligands (Garlatti, V., Belloy, N., Martin, L., Lacroix, M., Matsushita, M., Endo, Y., Fujita, T., Fontecilla-Camps, J. C., Arlaud, G. J., Thielens, N. M., and Gaboriaud, C. (2007) EMBO J. 24, 623-633). We now report the ligand-bound crystal structures of the recognition domain of M-ficolin, determined at high resolution (1.75-1.8 A), which provides the first structural insights into its binding properties. Interaction with acetylated carbohydrates differs from the one previously described for L-ficolin. This study also reveals the structural determinants for binding to sialylated compounds, a property restricted to human M-ficolin and its mouse counterpart, ficolin B. Finally, comparison between the ligand-bound structures obtained at neutral pH and nonbinding conformations observed at pH 5.6 reveals how the ligand binding site is dislocated at acidic pH. This means that the binding function of M-ficolin is subject to a pH-sensitive conformational switch. Considering that the homologous ficolin B is found in the lysosomes of activated macrophages (Runza, V. L., Hehlgans, T., Echtenacher, B., Zahringer, U., Schwaeble, W. J., and Mannel, D. N. (2006) J. Endotoxin Res. 12, 120-126), we propose that this switch could play a physiological role in such acidic compartments. 相似文献
45.
Structural and thermodynamic bases for the design of pure prolactin receptor antagonists: X-ray structure of Del1-9-G129R-hPRL 总被引:2,自引:0,他引:2
Jomain JB Tallet E Broutin I Hoos S van Agthoven J Ducruix A Kelly PA Kragelund BB England P Goffin V 《The Journal of biological chemistry》2007,282(45):33118-33131
Competitive antagonists of the human prolactin (hPRL) receptor are a novel class of molecules of potential therapeutic interest in the context of cancer. We recently developed the pure antagonist Del1-9-G129R-hPRL by deleting the nine N-terminal residues of G129R-hPRL, a first generation partial antagonist. We determined the crystallographic structure of Del1-9-G129R-hPRL, which revealed no major change compared with wild type hPRL, indicating that its pure antagonistic properties are intrinsically due to the mutations. To decipher the molecular bases of pure antagonism, we compared the biological, physicochemical, and structural properties of numerous hPRL variants harboring N-terminal or Gly(129) mutations, alone or combined. The pure versus partial antagonistic properties of the multiple hPRL variants could not be correlated to differences in their affinities toward the hPRL receptor, especially at site 2 as determined by surface plasmon resonance. On the contrary, residual agonism of the hPRL variants was found to be inversely correlated to their thermodynamic stability, which was altered by all the Gly(129) mutations but not by those involving the N terminus. We therefore propose that residual agonism can be abolished either by further disrupting hormone site 2-receptor contacts by N-terminal deletion, as in Del1-9-G129R-hPRL, or by stabilizing hPRL and constraining its intrinsic flexibility, as in G129V-hPRL. 相似文献
46.
The human Nup107-160 nuclear pore subcomplex contributes to proper kinetochore functions 总被引:2,自引:0,他引:2 下载免费PDF全文
Zuccolo M Alves A Galy V Bolhy S Formstecher E Racine V Sibarita JB Fukagawa T Shiekhattar R Yen T Doye V 《The EMBO journal》2007,26(7):1853-1864
We previously demonstrated that a fraction of the human Nup107-160 nuclear pore subcomplex is recruited to kinetochores at the onset of mitosis. However, the molecular determinants for its kinetochore targeting and the functional significance of this localization were not investigated. Here, we show that the Nup107-160 complex interacts with CENP-F, but that CENP-F only moderately contributes to its targeting to kinetochores. In addition, we show that the recruitment of the Nup107-160 complex to kinetochores mainly depends on the Ndc80 complex. We further demonstrate that efficient depletion of the Nup107-160 complex from kinetochores, achieved either by combining siRNAs targeting several of its subunits excluding Seh1, or by depleting Seh1 alone, induces a mitotic delay. Further analysis of Seh1-depleted cells revealed impaired chromosome congression, reduced kinetochore tension and kinetochore-microtubule attachment defects. Finally, we show that the presence of the Nup107-160 complex at kinetochores is required for the recruitment of Crm1 and RanGAP1-RanBP2 to these structures. Together, our data thus provide the first molecular clues underlying the function of the human Nup107-160 complex at kinetochores. 相似文献
47.
This is the first detailed report of social factors affecting fish-eating in Japanese macaques under natural circumstances. We video-recorded a complete event of fish eating, involving a new fish food species for the monkeys on Koshima island. Following the discovery of a large beached sea bass by a peripheral male, we observed a total of 16 individuals feeding on the fish in turns, and interacting around it. The rank order of access to the fish was mainly explained by the spatial position of group members, whereas dominance determined how long the fish was monopolized. Although limited, the tolerated presence of close-bystanders while feeding was affected by kinship and affiliation. Genealogic data suggested that fish-eating behavior was well maintained in terms of maternal lineages. This report should contribute to a better understanding of how social features may constrain the long-term diffusion of feeding innovations in free-ranging primate groups. 相似文献
48.
The checkpoint Saccharomyces cerevisiae Rad9 protein contains a tandem tudor domain that recognizes DNA 总被引:1,自引:1,他引:0
Lancelot N Charier G Couprie J Duband-Goulet I Alpha-Bazin B Quémeneur E Ma E Marsolier-Kergoat MC Ropars V Charbonnier JB Miron S Craescu CT Callebaut I Gilquin B Zinn-Justin S 《Nucleic acids research》2007,35(17):5898-5912
DNA damage checkpoints are signal transduction pathways that are activated after genotoxic insults to protect genomic integrity. At the site of DNA damage, ‘mediator’ proteins are in charge of recruiting ‘signal transducers’ to molecules ‘sensing’ the damage. Budding yeast Rad9, fission yeast Crb2 and metazoan 53BP1 are presented as mediators involved in the activation of checkpoint kinases. Here we show that, despite low sequence conservation, Rad9 exhibits a tandem tudor domain structurally close to those found in human/mouse 53BP1 and fission yeast Crb2. Moreover, this region is important for the resistance of Saccharomyces cerevisiae to different genotoxic stresses. It does not mediate direct binding to a histone H3 peptide dimethylated on K79, nor to a histone H4 peptide dimethylated on lysine 20, as was demonstrated for 53BP1. However, the tandem tudor region of Rad9 directly interacts with single-stranded DNA and double-stranded DNAs of various lengths and sequences through a positively charged region absent from 53BP1 and Crb2 but present in several yeast Rad9 homologs. Our results argue that the tandem tudor domains of Rad9, Crb2 and 53BP1 mediate chromatin binding next to double-strand breaks. However, their modes of chromatin recognition are different, suggesting that the corresponding interactions are differently regulated. 相似文献
49.
Véron JB Enguehard-Gueiffier C Snoeck R Andrei G De Clercq E Gueiffier A 《Bioorganic & medicinal chemistry》2007,15(22):7209-7219
The synthesis of original imidazo[1,2-a]pyridines bearing a phenethylthiomethyl side chain at the 3 position and a (hetero)aryl substituent on the 6 or 8 position, and their antiviral activities are reported. From the synthesized compounds, the 6-halogeno and 6-phenylimidazo[1,2-a]pyridine derivatives 4c-d and 5b were the most potent against human cytomegalovirus (CMV) and/or varicella-zoster virus (VZV), whereas several other congeners (i.e., 5e, 5g, 5i, 5l, 5n, 5p, 5q, and 5t), while less potent, were equally or more selective in their inhibitory activity against both VZV and CMV. These compounds showed similar activity against thymidine kinase competent (TK(+)) and deficient (TK(-)) VZV strains, demonstrating a mechanism of action independent of the viral thymidine kinase. 相似文献
50.
Induced parthenogenesis in mandarin for haploid production: induction procedures and genetic analysis of plantlets 总被引:1,自引:0,他引:1
Froelicher Y Bassene JB Jedidi-Neji E Dambier D Morillon R Bernardini G Costantino G Ollitrault P 《Plant cell reports》2007,26(7):937-944
This study focused on haploid induction in mandarin through in situ gynogenesis by pollination with irradiated pollen of ‘Meyer’
lemon. Pollination was carried out for three genotypes of mandarin with four levels of gamma-ray-irradiated pollen (150, 300,
600, and 900 Gy). The resulting seeds were characterised by a small size. Embryos were rescued in vitro and the ploidy level
of the plantlets was determined by flow cytometry analysis. Haploid, diploid, triploid plantlets were obtained. The haploid
parthenogenetic origin was confirmed using microsatellite marker analysis and chromosome count. Diploid and triploid plants
were the result of crosses between mandarin and lemon. The induction of gynogenetic haploids of ‘Fortune’ (Citrus clementina Hort ex Tan. × Citrus tangerina Hort ex Tan.) and ‘Ellendale’ (Citrus reticulata Blanco × Citrus sinensis L. Osb) is reported here for the first time. 相似文献