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Bernardine Presnell Amedeo Conti Georg Erhardt Ingolf Krause Jasminka Godovac-Zimmermann 《Journal of biochemical and biophysical methods》1990,20(4):325-333
A rapid method for determination of the primary structures for β-lactoglobulin (β-LG) genetic variants is described. This included rapid microbore HPLC, amino acid analyses, and wherever necessary, direct peptide sequencing. Two novel variants of β-LG have been identified, bovine β-LG W and ovine β-LG C. The proteins were oxidized, digested with trypsin and separated using RP-HPLC. All peptides were recovered in a single run. Peptides with amino acid exchanges were identified by retention time and subjected to amino acid and sequence analyses. Ovine β-LG C differs from the ovine β-LG A variant by a single amino acid exchange at position 148 where Arg is replaced by Gln. Bovine β-LG W differs from bovine β-LG B by having Leu at position 56 instead of Ile. The method described here is reliable and can be used for mapping of 20–1000 pmol of material. 相似文献
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Expression, signaling, and function of P2X7 receptors in bone 总被引:1,自引:0,他引:1
Matthew W. Grol Nattapon Panupinthu Jasminka Korcok Stephen M. Sims S. Jeffrey Dixon 《Purinergic signalling》2009,5(2):205-221
Nucleotides released from cells in response to mechanical stimulation or injury may serve as paracrine regulators of bone cell function. Extracellular nucleotides bind to multiple subtypes of P2 receptors on osteoblasts (the cells responsible for bone formation) and osteoclasts (cells with the unique ability to resorb mineralized tissues). Both cell lineages express the P2X7 receptor subtype. The skeletal phenotype of mice with targeted disruption of P2rx7 points to interesting roles for this receptor in the regulation of bone formation and resorption, as well as the response of the skeleton to mechanical stimulation. This paper reviews recent work on the expression of P2X7 receptors in bone, their associated signal transduction mechanisms and roles in regulating bone formation and resorption. Areas for future research in this field are also discussed. 相似文献