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Matthew W Blair Sharon JB Knewtson Carolina Astudillo Chee-Ming Li Andrea C Fernandez Michael A Grusak 《BMC plant biology》2010,10(1):215
Background
Iron deficiency anemia is a global problem which often affects women and children of developing countries. Strategy I plants, such as common bean (Phaseolus vulgaris L.) take up iron through a process that involves an iron reduction mechanism in their roots; this reduction is required to convert ferric iron to ferrous iron. Root absorbed iron is critical for the iron nutrition of the plant, and for the delivery of iron to the shoot and ultimately the seeds. The objectives of this study were to determine the variability and inheritance for iron reductase activity in a range of genotypes and in a low × high seed iron cross (DOR364 × G19833), to identify quantitative trait loci (QTL) for this trait, and to assess possible associations with seed iron levels. 相似文献103.
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The sequences of the linked alpha 2- and alpha 1-globin genes of the equine
BI and BII haplotypes are greater than 99% identical within a 1.2-kb region
extending from approximately 75 bp upstream of the putative cap site to a
point approximately 150 bp 3' to the poly A addition signal. Differences
between the alpha 2 and alpha 1 genes that are common to both haplotypes
indicate that a major gene conversion occurred approximately 12 Myr ago and
that this has been followed by shorter, more localized, conversions.
Interhaplotype (allelic) comparisons at the alpha loci suggest that the BI
and BII haplotypes have probably existed independently greater than or
equal to 0.5 Myr and that the alpha 1 genes may have undergone a recent
interchromosomal gene conversion.
相似文献
106.
Mycoplasmas are notorious contaminants of cell culture and can have profound effects on host cell biology by depriving cells of nutrients and inducing global changes in gene expression. Over the last two decades, sentinel testing has revealed wide-ranging contamination rates in mammalian culture. To obtain an unbiased assessment from hundreds of labs, we analyzed sequence data from 9395 rodent and primate samples from 884 series in the NCBI Sequence Read Archive. We found 11% of these series were contaminated (defined as ≥100 reads/million mapping to mycoplasma in one or more samples). Ninety percent of mycoplasma-mapped reads aligned to ribosomal RNA. This was unexpected given 37% of contaminated series used poly(A)-selection for mRNA enrichment. Lastly, we examined the relationship between mycoplasma contamination and host gene expression in a single cell RNA-seq dataset and found 61 host genes (P < 0.001) were significantly associated with mycoplasma-mapped read counts. In all, this study suggests mycoplasma contamination is still prevalent today and poses substantial risk to research quality. 相似文献
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