Effect of Light on Deamination and Oxidation of Adenylic Compounds in Cotyledons of Pharbitis nil

Since labelling of ureides from adenine-8-¹⁴C is higher in dark than in light, the influence of light on the deamination and the oxidation of adenylic compounds by cotyledon discs of Pharbitis nit was investigated. Among the three possible adenylic precursors for the deaminative step, adenine was found to be the best compound for the study of the deaminative rate, adenosine being easily hydrolyzed into adenine, and AMP undergoing an apparent complete hydrolysis before entering the cells. By analysis of adenine-8-¹⁴C metabolism for brief periods, it was determined that the rate of deamination of adenylic compounds was faster in light than in dark. In contrast, the activity of xanthine dehydrogenase was much higher in the dark than in light. The level of the activity of uricase was the same in both light and dark.     

Carbon Dioxide and pH Requirements of Non-Photosynthetic Tissue Culture Cells

Maximum growth of suspension cultures of Paul's Scarlet rose required a low pH (5.2 to 5.4) during the division phase (day 0 to 7) and a higher pH (5.8 to 6.0) during the expansion phase (day 7 to 14). The fresh weight increase was reduced by approximately 22%, but the dry weight was not influenced when cells were grown for 14 days in a CO₂ deficient environment. Kinetic studies showed that the first five days of growth was the critical period of nonautotrophic CO₂ fixation when cells were grown in medium buffered at pH 5.4. The phosphoenolpyruvate carboxylase activity was highest (0.50 × 10⁶ cpm min^?1· g^?1 fresh weight) during the period when nonautotrophic CO₂ fixation appeared to be critical for growth.     

Benthic ecology of a spring-fed river of interior Alaska

1. A massive aquifer between the Gerstle, Tanana and Delta rivers in interior Alaska receives water from them and from smaller streams that flow from the Granite Mountains in the Alaska Range, Groundwater from the aquifer intersects the surface in a mid-sized (20m³s^?1× 10%) spring-fed stream, Clearwater Creek. 2. Mean annual air temperature is about -2.6°C. However, even in winter when air temperature often reaches —40°C, the stream does not form a complete ice cover. Water temperature ranges from 0 to 7.8°C. Specific conductance and the concentrations of major ions vary little throughout the year, and summed ionic salinity exceeds 250 mg1-^?1. 3. Benthic algal standing crop (as chlorophyll a) was at least an order of magnitude higher than that in a nearby surface-water stream, the upper Chena River, Standing crop peaked in spring and autumn (about 20mgm^?2) and averaged about half this value, although biomass of an early spring bloom of Hydrurus foetid us was underestimated. 4. Algal standing crop was inversely related to the concentrations of inorganic nitrogen and orrhophosphate-phosphorus in the water column. The ratio of total nitrogen to total phosphorus (as mass concentrations) was always about 30. Measurements of primary production made in Clearwater Creek were among the highest reported for streams in subarctic Alaska. 5. Macroinvertebrate diversity in Clearwater Creek was low. Numbers of ‘morpho-species’ in monthly Surber samples (0.09m²) averaged nine, and ranged from three to fourteen. However, benthos and drift densities were similar to those reported from other Alaskan streams. In early spring and autumn, drifting macroinvertebrates were primarily Ephemeroptera, Plecoptera and Trichoptera, but in summer, Diptera dominated the drift. The low diversity of macroinvertebrates is hypothesized to be a consequence of the small annual range in water temperature and the relatively constant discharge of Clearwater Creek.     

Role of β1 Integrins in Cell Spreading and Migration of Human Nevomelanocytes and Dysplastic Nevi Cells on Collagen Type IV and Laminin

We characterized β1 integrin subunit expression on three different cultures of benign human nevomelanocytes (NMC) and on four different cell cultures of human dysplastic nevus (DN) cells by flow cytometry analysis and examined their role in mediating cell spreading and migration on collagen type IV (CN IV) and laminin (LN) coated substrates by using a quantitative video image analysis system. The seven human NMC and DNC cultures expressed heterogeneous levels of β1, α2, α3 and α6 integrin subunits. Image analysis showed that a significant increase (P<0.001) in cell spreading and migration of the DN cells was induced on increasing coating concentrations of CN IV and LN. However, the NMC did not show an increase in cell spreading or migration on these substrates when compared to the substrates coated with denatured BSA only. The CN IV-induced cell spreading of the DN cells was significantly inhibited by anti-β1 mAb (AIIB2), anti-α2 mAb (P1E6), or anti-α3 mAb (P1B5), but not by mAb against α6 integrin subunit (GoH3). The DN cell spreading on LN was not significantly inhibited by these mAbs. In contrast, the migration of the DN on CN IV and LN was significantly inhibited by anti-β1 mAb, anti-α2 mAb, anti-α3 mAb and anti-α6 mAb. These data suggest that the α2 and α3 subunit are important for cell spreading of the DN on CN IV, although they are less important in cell spreading on the extracellular matrix component LN. The α2, α3 and α6 integrin subunits are important for the migration of DN cells on both CN IV and LN.     

Pre-flowering and post-flowering insecticide applications to control Aphis fabae on field beans: their biological and economic effectiveness

Most spring-sown field beans are treated with insecticides to control Aphis fabae, but there is usually a risk of toxicity to pollinating honeybees if insecticides are applied during flowering. An experiment was repeated at Rothamsted for 6 consecutive years, comparing treatments before and after flowering. It is concluded that (1) pre-flowering treatments (demeton-S-methyl spray at 0–25 kg a.i. ha^-1 or phorate granules at 1–12 kg a.i. ha^-1) were more effective and profitable than post-flowering treatments, (2) application of phorate granules before flowering gave the greatest economic return, and minimised the danger to foraging bees and (3) aphid populations varied considerably from year to year, as did the relationship between aphid infestation and yield, but our evidence supports the suggestion that insecticide spray treatments are profitable if 5% or more of the plant stems are infested at the end of primary aphid migration.     

The epidemiology of tomato mosaic: Sources of TMV in commercial tomato crops under glass

Of the several possible sources of tomato mosaic virus, seeds and root debris in the soil are considered to be of greatest importance. A survey of 374,000 seedlings on ten commercial holdings found 0.05% of them infected, and although these were removed virus had been spread to other young plants which did not show infection when transplanted into the growing houses, seven of twenty-two of which contained a few infected plants when sampled shortly after planting. Virus overwintering on clothing, and debris on structures, are thought to be of minor importance, and smoking tobacco is seldom a source of infection for the tomato crop. A further survey of seventy-eight samples from tomato crops in Britain confirmed the 1960-61 survey: all were infected with tomato strains of TMV, none with tobacco strains, but one of the 187 infected seedlings referred to above was carrying a tobacco strain. Petunia was not as satisfactory as a special cultivar of White Burley tobacco for distinguishing between the tobacco and tomato TMV isolates. Observations and tests on a commercial holding showed that TMV was readily carried from plants in infected glasshouses into clean ones by workers, and once introduced, spread rapidly within the crop.