全文获取类型
收费全文 | 720篇 |
免费 | 34篇 |
出版年
2021年 | 5篇 |
2017年 | 4篇 |
2016年 | 4篇 |
2015年 | 5篇 |
2014年 | 8篇 |
2013年 | 71篇 |
2012年 | 30篇 |
2011年 | 28篇 |
2010年 | 19篇 |
2009年 | 16篇 |
2008年 | 36篇 |
2007年 | 49篇 |
2006年 | 40篇 |
2005年 | 40篇 |
2004年 | 57篇 |
2003年 | 53篇 |
2002年 | 39篇 |
2001年 | 14篇 |
2000年 | 8篇 |
1999年 | 9篇 |
1998年 | 9篇 |
1997年 | 7篇 |
1996年 | 9篇 |
1995年 | 8篇 |
1994年 | 13篇 |
1993年 | 13篇 |
1992年 | 6篇 |
1991年 | 7篇 |
1990年 | 9篇 |
1989年 | 12篇 |
1988年 | 5篇 |
1987年 | 3篇 |
1986年 | 4篇 |
1985年 | 8篇 |
1984年 | 13篇 |
1982年 | 10篇 |
1981年 | 14篇 |
1980年 | 7篇 |
1979年 | 7篇 |
1978年 | 4篇 |
1977年 | 5篇 |
1976年 | 3篇 |
1975年 | 5篇 |
1974年 | 5篇 |
1973年 | 7篇 |
1972年 | 4篇 |
1970年 | 3篇 |
1969年 | 3篇 |
1967年 | 4篇 |
1966年 | 3篇 |
排序方式: 共有754条查询结果,搜索用时 484 毫秒
751.
A sensitive assay system of Yaba virus (YV) was established in a cynomolgus monkey kidney cell line, JINET, in which the virus caused multilayered cellular foci countable even with the unaided eye. The specificity of the foci induced by YV in these cells was demonstrated by (1) the focus-forming ability was destroyed by heating at 60 C for 12 min; (2) the focus formation was inhibited by specific antiserum; (3) specific fluorescence was detected only in cells composing the foci when tested by fluorescent antibody technique; (4) a linear relationship was observed between the virus concentration and the number of foci formed; (5) YV preparation passed 20 times in JINET cells still possessed “tumorigenicity” in cynomolgus monkeys. The sensitivity of JINET cells to YV was comparable to that of cynomolgus monkeys, and YV was successively propagated in JINET cells with 2 log increase in infectivity titer during over 40 serial passages. Application of this assay system to growth kinetic studies of YV and quantitation of neutralizing antibody to YV is also discussed. 相似文献
752.
An anchorage-dependent Wilms’ tumor cell line, HFWT, has been found to be extremely susceptible to human natural killer (NK) cells. Here we established a transfectant of HFWT with the green fluorescence protein (GFP) gene, designated GHINK-1 cells, to apply to the activated NK cytotoxicity assay without radioisotope labeling. After being co-cultured with CD3?CD56+ NK cells, GHINK-1 cells released GFP into the medium. The intensity of the fluorescence from the released GFP correlated almost exactly with the radioactivity of a standard 51Cr-release assay performed with suspension-cultured K562 cells. Because it does not require separation of the remaining live target cells by centrifugation, the non-radioisotopic GFP release assay with GHINK-1 cells is a convenient alternative for monitoring human activated NK killing activity. 相似文献
753.
Isamu Murakoshi Eiji Kidoguchi Mohammad Ikram Mohammad Israr Nusrat Shafi Joju Haginiwa Shigeru Ohmiya Hirotaka Otomasu 《Phytochemistry》1982,21(6):1313-1315
The major alkaloids of Sophora mollis are (+)-sparteine and (?)-cytisine, and the minor ones are also of the sparteine-type (lupanine and 5,6-deh 相似文献
754.
Isamu Murakoshi Eiji Kidoguchi Joju Haginiwa Shigeru Ohmiya Kimio Higashiyama Hirotaka Otomasu 《Phytochemistry》1982,21(9):2379-2384
Two new lupin alkaloids, isokuraramine and (?)-7, 11-dihydromatrine, were isolated from the fresh flowers of Sophora flavescens along with 16 kno 相似文献