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991.
Héctor?J.?CarloEmail author Iris?F.?A.?Vis Kees?Jan?Roodbergen 《Flexible Services and Manufacturing Journal》2015,27(2-3):224-262
Seaside operations are considered the bottleneck operation in most container terminals around the world. This paper presents an in-depth updated overview of the seaside operations at container terminals and highlights current trends and developments. We review and classify scientific journal papers on container terminal seaside operations, published between 2004 and 2012. The paper also discusses and challenges the current operational paradigms on seaside operations. Lastly, the paper identifies new avenues for academic research based on current trends and developments in the container terminal industry. 相似文献
992.
Dhayana Dallmeier Michael J. Pencina Iris Rajman Wolfgang Koenig Dietrich Rothenbacher Hermann Brenner 《PloS one》2015,10(1)
Objective
To assess the prognostic value of 12-months N-Terminal Pro-Brain Natriuretic Peptide (NT-proBNP) levels on adverse cardiovascular events in patients with stable coronary heart disease.Methods
NT-proBNP concentrations were measured at baseline and at 12-months follow-up in participants of cardiac rehabilitation (median follow-up 8.96 years). Cox-proportional hazards models evaluated the prognostic value of log-transformed NT-proBNP levels, and of 12-months NT-proBNP relative changes on adverse cardiovascular events adjusting for established risk factors measured at baseline.Results
Among 798 participants (84.7% men, mean age 59 years) there were 114 adverse cardiovascular events. 12-months NT-proBNP levels were higher than baseline levels in 60 patients (7.5%) and numerically more strongly associated with the outcome in multivariable analysis (HR 1.65 [95% CI 1.33–2.05] vs. HR 1.41 [95% CI 1.12–1.78], with a net reclassification improvement (NRI) of 0.098 [95% CI 0.002–0.194] compared to NRI of 0.047 [95% CI −0.0004–0.133] for baseline NT-proBNP levels. A 12-month 10% increment of NT-proBNP was associated with a HR of 1.35 [95% CI 1.12–1.63] for the onset of an adverse cardiovascular event. Subjects with a 12-month increment of NT-proBNP had a HR of 2.56 [95% CI 1.10–5.95] compared to those with the highest 12-months reduction.Conclusions
Twelve-months NT-proBNP levels after an acute cardiovascular event are strongly associated with a subsequent event and may provide numerically better reclassification of patients at risk for an adverse cardiovascular event compared to NT-proBNP baseline levels after adjustment for established risk factors. 相似文献993.
994.
Anna Aulinas María-José Ramírez María-José Barahona Elena Valassi Eugenia Resmini Eugènia Mato Alicia Santos Iris Crespo Olga Bell Jordi Surrallés Susan M. Webb 《PloS one》2015,10(3)
Introduction
Cushing’s syndrome (CS) increases cardiovascular risk (CVR) and adipocytokine imbalance, associated with an increased inflammatory state. Telomere length (TL) shortening is a novel CVR marker, associated with inflammation biomarkers. We hypothesized that inflammatory state and higher CVR in CS might be related to TL shortening, as observed in premature aging.Aim
To evaluate relationships between TL, CVR and inflammation markers in CS.Methods
In a cross-sectional study, 77 patients with CS (14 males, 59 pituitary-, 17 adrenal- and 1 ectopic-origin; 21 active disease) and 77 age-, gender-, smoking-matched controls were included. Total white blood cell TL was measured by TRF-Southern technique. Clinical data and blood samples were collected (lipids, adrenal function, glucose). Adiponectin, interleukin-6 (IL6) and C-reactive protein (CRP) were available in a subgroup of patients (n=32). Correlations between TL and clinical features were examined and multiple linear regression analysis was performed to investigate potential predictors of TL.Results
Dyslipidemic CS had shorter TL than non-dyslipidemic subjects (7328±1274 vs 7957±1137 bp, p<0.05). After adjustment for age and body mass index, cured and active CS dyslipidemic patients had shorter TL than non-dyslipidemic CS (cured: 7187±1309 vs 7868±1104; active: 7203±1262 vs 8615±1056, respectively, p<0.05). Total cholesterol and triglycerides negatively correlated with TL (r-0.279 and -0.259, respectively, p<0.05), as well as CRP and IL6 (r-0.412 and -0.441, respectively, p<0.05). No difference in TL according the presence of other individual CVR factors (hypertension, diabetes mellitus, obesity) were observed in CS or in the control group. Additional TL shortening was observed in dyslipidemic obese patients who were also hypertensive, compared to those with two or less CVR factors (6956±1280 vs 7860±1180, respectively, p<0.001). Age and dyslipidemia were independent negative predictors of TL.Conclusion
TL is shortened in dyslipidemic CS patients, further worse if hypertension and/or obesity coexist and is negatively correlated with increased inflammation markers. Increased lipids and a “low” grade inflammation may contribute to TL shortening and consequently to premature ageing and increased morbidity in CS. 相似文献995.
Pfenninger C Leinhase I Endres M Rotter N Loch A Ringe J Sittinger M 《In vitro cellular & developmental biology. Animal》2007,43(1):28-36
The reconstruction of extensive tracheal defects is still an unsolved challenge for thoracic surgery. Tissue engineering is
a promising possibility to solve this problem through the generation of an autologous tracheal replacement from patients’
own tissue. Therefore, this study investigated the potential of three different coculture systems, combining human respiratory
epithelial cells and human chondrocytes. The coculture systems were analyzed by histological staining with alcian blue, immunohistochemical
staining with the antibodies, 34betaE12 and CD44v6, and scanning electron microscopy. The first composite culture consisted
of human respiratory epithelial cells seeded on human high-density chondrocyte pellets. For the second system, we used native
articular cartilage chips as base for the respiratory epithelial cells. The third system consisted of a collagen membrane,
seeded with respiratory epithelial cells and human chondrocytes onto different sides of the membrane, which achieved the most
promising results. In combination with an air–liquid interface system and fibroblast-conditioned medium, an extended epithelial
multilayer with differentiated epithelial cells could be generated. Our results suggest that at least three factors are necessary
for the development towards a tracheal replacement: (1) a basal lamina equivalent, consisting of collagen fibers for cell–cell
interaction and cell polarization, (2) extracellular factors of mesenchymal fibroblasts, and (3) the presence of an air–liquid
interface system for proliferation and differentiation of the epithelial cells. 相似文献
996.
Valdés I Hermida L Zulueta A Martín J Silva R Alvarez M Guzmán MG Guillén G 《Molecular biotechnology》2007,35(1):23-30
Among the Dengue virus structural proteins, the Envelope glycoprotein is the most important because of its antigenic characteristics.
In this work, the E protein from Dengue-2 virus truncated at the C-terminus region was successfully expressed in Pichia pastoris. The E2trunc gene was cloned under the AOX1 promoter from P. pastoris and the signal peptide of the sucrose invertase gene from Saccharomyces cerevisiae. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis of expression revealed the presence of a protein with
the expected size, which was completely associated to the insoluble fraction after cellular disruption. The recombinant N-glycosylated protein reacted with two conformational antibodies against Dengue-2, indicating a proper folding of it. In addition,
it was able to induce antiviral antibodies after mice immunization. 相似文献
997.
998.
999.
Eliahu D Aflalo Uriel A Sod-Moriah Gad Potashnik Iris Har-Vardi 《Reproductive biology and endocrinology : RB&E》2007,5(1):1-11
Background
Embryo implantation plays a major role in embryogenesis and the outcome of pregnancy. Plasminogen activators (PAs) have been implicated in mammalian fertilization, early stages of development and embryo implantation. As in-vitro developing embryos resulted in lower implantation rate than those developed in-vivo we assume that a reduced PAs activity may be involved. In the present work we studied the effect of EGF on PAs activity, quantity and embryo implantation.Methods
Zygotes were flushed from rat oviducts on day one of pregnancy and grown in-vitro in R1ECM supplemented with EGF (10 ng/ml) and were grown up to the blastocyst stage. The control groups were grown in the same medium without EGF. The distribution and quantity of the PAs were examined using fluorescence immunohistochemistry followed by measurement of PAs activity using the chromogenic assay. Implantation rate was studied using the embryo donation model.Results
PAs distribution in the embryos was the same in EGF treated and untreated embryos. Both PAs were localized in the blastocysts' trophectoderm, supporting the assumption that PAs play a role in the implantation process in rats. EGF increased the quantity of uPA at all stages studied but the 8-cell stage as compared with controls. The tissue type PA (tPA) content was unaffected except the 8-cell stage, which was increased. The activity of uPA increased gradually towards the blastocyst stage and more so due to the presence of EGF. The activity of tPA did not vary with the advancing developmental stages although it was also increased by EGF. The presence of EGF during the preimplantation development doubled the rate of implantation of the treated group as compared with controls. 相似文献1000.