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121.
Protein p0071, a major plaque protein of non-desmosomal adhering junctions,is a selective cell-type marker 总被引:1,自引:1,他引:0
Protein p0071, which originally was introduced as a member of the p120-subfamily of armadillo proteins, common to desmosomes and adhaerens junctions (AJs) and to several other cell structures (centrosomes, midbodies), has been localized by using a series of novel
mono- and polyclonal antibodies generated against various domains of the molecule. By protein analysis and immunolocalization
techniques, protein p0071 has been localized as a plaque protein in AJs of diverse epithelia and certain vascular endothelia,
in the composite junctions (areal compositae) of the intercalated disks of cardiomyocytes, and in the punctate or more extended AJs of the vast majority of cell culture
types examined, including mitotic states. Using these antibodies, we have also shown that this AJ protein occurs only rarely
or is even absent in tissues such as skeletal and smooth muscles, in a series of mesenchymal tissue cells, and in specific
desmosome-rich cells such as those of the upper layers of the epidermis and certain other stratified epithelia and Hassall
corpuscles of the thymus. We have also demonstrated that p0071 is absent from desmosomes. The occurrence of two major subtypes
of lymphatic endothelial cells, one with AJs containing p0071 and one without detectable p0071, is emphasized. Possible structural
and functional roles of p0071 are discussed in light of these new findings regarding its localization, and the addition of
p0071 to the armamentarium of cytodiagnostic cell-type markers is recommended.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users.
This study was supported by project grants from the German Ministry for Education and Research (BMBF) in a cooperative research
program entitled “Standardization of mesenchymal stem cells for regenerative medicine (START-MSC)” and from the Deutsche Krebshilfe
(project 10–2049) to W.W. Franke. 相似文献
122.
123.
Cytogenetic evolution of human ovarian cell lines associated with chemoresistance and loss of tumorigenicity. 总被引:1,自引:0,他引:1
Stéphanie Struski Martine Doco-Fenzy Michael Koehler Ilse Chudoba Francis Levy Linda Masson Nicole Michel Evelyne Ulrich Nadine Gruson Jean Bénard Gérard Potron Pascale Cornillet-Lefebvre 《Analytical cellular pathology》2003,25(3):115-122
In order to identify genomic changes associated with a resistant phenotype acquisition, we used comparative genomic hybridization (CGH) to compare a human ovarian cell line, Igrov1, and four derived subcell lines, resistant to vincristine and presenting a reversion of malignant properties. Multicolor FISH (Multiplex-FISH and Spectral Karyotype) and conventional FISH are also used to elucidate the karyotype of parental cell line. The drug-resistant subcell lines displayed many chromosomal abnormalities suggesting the implication of different pathways leading to a multidrug resistance phenotype. However, these cell lines shared two common rearrangements: an unbalanced translocation der(8)t(8;13)(p22;q?) and a deletion of the 11p. These chromosomal imbalances could reflected the acquisition of the chemoresistance (der(8)) or the loss of tumorigenicity properties (del(11p)). 相似文献
124.
125.
Ilse Schwinck 《Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology》1953,35(3):167-174
Zusammenfassung Der Weibchenduftstoff von Plodia interpunctella Hb. ist, entgegen den Angaben Barths, auch bei Zimmertemperatur sehr stabil; geringe Mengen (von 2 in 4 min abgegebener Duftstoff) konnten nach über 2 Tagen in einer geschlossenen Schale durch den Schwirrtanz der Männchen noch nachgewiesen werden.Zur Frage der Artspezifität der Sexualduftstoffe zeigten Versuche in geschlossenen Schalen, im freien Flugkäfig und in luftdurchströmten Apparaturen für die beiden nahe verwandten Gattungen Plodia interpunctella Hb. und Ephestia kühniella Z. keine Spezifität des Erregungs und Anlockungsvorganges, zu einer Kopulation zwischen gattungsfremden Tieren kam es aber trotz wilder Kopulationsversuche nicht. 相似文献
126.
Cornelia Speth Jörg T. Epplen Ilse Oberbäumer 《In vitro cellular & developmental biology. Animal》1991,27(8):646-650
Summary Oligonucleotide fingerprinting was applied to investigate the relatedness of several cell lines that were established between
1973 and 1977 from a teratocarcinoma. We were able to distinguish cell lines derived at different times. In addition, sublines
from one cell line (PYS-2) could be discriminated by using a combination of different probes. Therefore multilocus fingerprinting
with oligonucleotides is a useful method for monitoring changes in cell lines kept in culture for many generations.
This work was supported by the Deutsche Forschungsgemeinschaft (OB 66/2-1) and by the VW-Stiftung. 相似文献
127.
128.
In the spore germination test the germination rate of ALTERNARIA TENUIS Neerg on serum-agar with serum from rats exposed to 3,450 m altitude was significently higher than with serum from rats at ground level. The responsible factor has not yet been identified. 相似文献
129.
130.
Peter L Hagedoorn Martijn C.P.F Driessen Marieke van den Bosch Ilse Landa Wilfred R Hagen 《FEBS letters》1998,440(3):6683
The redox chemistry of Pyrococcus furiosus rubredoxin and ferredoxin has been studied as a function of temperature in direct voltammetry and in EPR monitored bulk titrations. The Ems of both proteins, measured with direct voltammetry, have a normal (linear) temperature dependence and show no pH dependence. EPR monitoring is not a reliable method to determine the temperature dependence of the Em: upon rapid freezing the proteins take their conformation corresponding to the freezing point of the solution. 相似文献