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71.
Bovine preantral follicles and activin: immunohistochemistry for activin and activin receptor and the effect of bovine activin A in vitro 总被引:1,自引:0,他引:1
Hulshof SC Figueiredo JR Beckers JF Bevers MM Vanderstichele H van den Hurk R 《Theriogenology》1997,48(1):133-142
Activin was originally isolated from follicular fluid as a factor stimulating FSH from the pituitary. Recent studies also suggest a local role for activin in the development of preantral and early antral follicles. In the present study, activin and activin receptor immunoreactivity are shown in oocyte and granulosa cells of bovine preantral follicles. In addition, activin immunoreactivity was observed in the theca of secondary follicles. During culture of isolated preantral follicles, activin increased follicular growth and granulosa cell proliferation in a dose-dependent manner. This increase was further stimulated by addition of FSH. In conclusion, activin and its receptor are present on bovine preantral follicles, and additional activin stimulates development of those follicles. 相似文献
72.
Prebiotic condensation reactions in an aqueous medium: A review of condensing agents 总被引:2,自引:0,他引:2
Biopolymers are formed by dehydration-type condensation reactions. In aqueous solutions dehydration reactions are very unlikely to happen spontaneously. However, coupling of dehydration-condensation to the hydrolysis of condensing agents could facilitate the synthesis of biopolymers in an aqueous solution. The literature shows that the peptides, nucleosides, nucleotides and oligonucleotides can be formed in this way. A careful study of the literature pertaining to prebiotic condensing agents was conducted in order to determine the most plausible prebiotic synthesis of biopolymers. The condensing agents taken into consideration are cyanamide, carbodiimide, dicyanamide, dicyandiamide, hydrogec-cyanide-tetramer, cyanogen and the linear- and cyclic polyphosphates. From both a chemical as well as biological point of view the polyphosphates appear to be the most plausible general prebiotic condensing agent. 相似文献
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Raphael B. Costa Gregório MF Camargo Iara DPS Diaz Natalia Irano Marina M. Dias Roberto Carvalheiro Arione A. Boligon Fernando Baldi Henrique N. Oliveira Humberto Tonhati Lucia G. Albuquerque 《遗传、选种与进化》2015,47(1)
Background
An important goal of Zebu breeding programs is to improve reproductive performance. A major problem faced with the genetic improvement of reproductive traits is that recording the time for an animal to reach sexual maturity is costly. Another issue is that accurate estimates of breeding values are obtained only a long time after the young bulls have gone through selection. An alternative to overcome these problems is to use traits that are indicators of the reproductive efficiency of the herd and are easier to measure, such as age at first calving. Another problem is that heifers that have conceived once may fail to conceive in the next breeding season, which increases production costs. Thus, increasing heifer’s rebreeding rates should improve the economic efficiency of the herd. Response to selection for these traits tends to be slow, since they have a low heritability and phenotypic information is provided only later in the life of the animal. Genome-wide association studies (GWAS) are useful to investigate the genetic mechanisms that underlie these traits by identifying the genes and metabolic pathways involved.Results
Data from 1853 females belonging to the Agricultural Jacarezinho LTDA were used. Genotyping was performed using the BovineHD BeadChip (777 962 single nucleotide polymorphisms (SNPs)) according to the protocol of Illumina - Infinium Assay II ® Multi-Sample HiScan with the unit SQ ™ System. After quality control, 305 348 SNPs were used for GWAS. Forty-two and 19 SNPs had a Bayes factor greater than 150 for heifer rebreeding and age at first calving, respectively. All significant SNPs for age at first calving were significant for heifer rebreeding. These 42 SNPs were next or within 35 genes that were distributed over 18 chromosomes and comprised 27 protein-encoding genes, six pseudogenes and two miscellaneous noncoding RNAs.Conclusions
The use of Bayes factor to determine the significance of SNPs allowed us to identify two sets of 42 and 19 significant SNPs for heifer rebreeding and age at first calving, respectively, which explain 11.35 % and 6.42 % of their phenotypic variance, respectively. These SNPs provide relevant information to help elucidate which genes affect these traits. 相似文献75.
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Environmental gradients have played a pivotal role in the history and development of plant ecology and are useful for testing ecological and evolutionary theory. Área de Conservación Guanacaste is a spatio-temporal mosaic of forests that have evolved continuously across elevation, topography, soil types, succession, and annual and inter-annual climatic change. Studies of plant ecology across diverse gradients of ACG have shaped functional ecology, successional theory, community assembly, plant–herbivore interactions, among many other fields. In this review, we synthesize the, perhaps overlooked, role plant ecological studies of ACG have had on our understanding of tropical forest dynamics. We outline present-day processes that will have major impacts on forest dynamics of ACG in the future and highlight how ACG will continue to shape future research priorities in plant ecology. 相似文献
77.
E. A. Zandvoort M. J. H. Hulshof G. Staritsky 《Plant Cell, Tissue and Organ Culture》1994,36(3):309-316
Xanthosoma germplasm requires regular subeulturing if stored in vitro under optimal culture conditions. An experiment was carried out to investigate whetherXanthosoma can be stored continuously under minimal growth conditions. Growth was reduced by incubation at low temperatures (9°C, 13°C and 17°C) and by adding mannitol (3.0% w/v) to the medium. Three investigatedXanthosoma species,Xanthosoma brasiliense, Xanthosoma robustum andXanthosoma sagittifolium could be stored in the dark for at least two years at 13°C. Addition of mannitol to the medium resulted in higher survival rates after three years of continuous storage. The storage period could be extended to at least 48 months forX. robustum andX. sagittifolium by annual subculture and regrowth under optimal culture conditions. 相似文献
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