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141.
C A Carothers Carraway H Fang X H Ye S H Juang Y C Liu M E Carvajal K L Carraway 《The Journal of biological chemistry》1991,266(24):16238-16246
[14C]Glucosamine metabolic labeling and concanavalin A blots were used to identify four major glycoprotein species associated with ascites tumor cell microvillar microfilament cores and with a transmembrane complex containing actin. Phalloidin shift analysis of glucosamine-labeled microvilli showed that glycoproteins of 110-120, 80, 65, and 55 kDa are stably associated with the microfilament cores. Analysis of large (greater than 10(6) kDa) transmembrane complexes from microvillar membranes made under microfilament-depolymerizing conditions (Carraway, C. A. C., Jung, G., and Carraway, K. L. (1983) Proc. Natl. Acad. Sci. U. S. A. 80, 430-434) revealed glycoproteins of the same Mr values, showing the same relative staining or labeling patterns as those observed with the microfilament cores. Gel filtration of high salt, high pH extracts of intact microvilli, microfilament cores, or transmembrane complexes showed that in all of these fractions the glycoproteins are associated in a very large, stable complex. The glycoprotein multimer was isolated essentially free of actin and other components by Sephacryl S-1000 chromatography of microvilli, microvillar membranes prepared at pH 11, microfilament cores, or transmembrane complex fractions in Triton X-100, 1 M KCl, glycine, pH 9.5. Purified glycoprotein complex bound actin when incubated under polymerizing conditions. The presence of the glycoprotein heteromultimer in both microfilament cores and transmembrane complex from isolated membranes and the association of the purified glycoprotein complex with actin are consistent with our hypothesis that the glycoprotein-containing transmembrane complex is an association site for microfilaments at the plasma membrane. 相似文献
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144.
本文探讨在外源性层粘连蛋白与抗癌药物顺铂的共同作用下,癌细胞内微丝组装的变化。结果发现外源性层粘连蛋白与小鼠腹水型肝癌细胞膜受体结合后,促进肌动蛋白微丝组装,使其含量增加;而多靶性抗癌药物顺铂与肌动蛋白微丝的结合,抑制微丝组装过程,造成微丝含量减少;两种试剂共同作用于癌细胞时,肌动蛋白微丝的含量与对照组相比非常接近。本研究为上述两种物质对癌细胞内微丝组装的拮抗性作用提出直接证据。 相似文献
145.
浙江省水鳖科植物的种类与分布 总被引:2,自引:0,他引:2
浙江省有水鳖科植物5属8种,均属淡水种类,其中无尾水筛、有尾水筛和密齿苦草为浙江分布新记录。在水平分布上,水筛属分布局限于浙江东南部,苦草属则分布于浙江大部分地区而未见于浙江南部。在垂直分布上,水筛属的种数和分布量,在海拔600米以下,随海拔增高而增加,再向上又有减少的趋势;其余各属的种数和分布量则随海拔增高而减少。它们的分布主要受各自适宜的生境在水平和垂直带的不同分布所制约。浙江水鳖科植物区系与我国亚热带各省区尤其是相邻省的关系十分密切。 相似文献
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147.
L Fang R J Knapp T Matsunaga S J Weber T Davis V J Hruby H I Yamamura 《Life sciences》1992,51(20):PL189-PL193
Both [D-Ala2,Glu4]Deltorphin and [D-Ala2,4'-I-Phe3,Glu4]Deltorphin are highly selective ligands for delta, relative to mu, opioid receptors. Radiolabeled [D-Ala2, 4'-125I-Phe3,Glu4]Deltorphin ([125I]Deltorphin) was prepared with a specific activity of 2200 Ci/mmol from [D-Ala2, 4'-NH2-Phe3, Glu4]Deltorphin through a diazonium salt intermediate. The inhibition of [125I]Deltorphin binding to rat brain membranes by ligands selective for mu, delta, and kappa opioid receptors is consistent with binding by the radioligand to a single site having the properties of a delta opioid receptor. The results of these studies are in good agreement with those obtained by structurally different delta opioid receptor ligands. The similarity between the delta receptor site labeled by [125I]Deltorphin and those labeled by other delta receptor agonists, in contrast to differences seen by in vivo studies of their analgesic effects, is discussed. 相似文献
148.
长白猪、枫泾猪和它们的杂种后代Ag—NOR的研究 总被引:11,自引:0,他引:11
4头枫泾猪,3头长白猪和5头长白×枫泾杂一代的NOR平均数分别为3.88、2和2.95。33头长白×枫泾杂二代猪(杂一代互交后代),其中7头黑猪的NOR众数为4,平均数为3.85;9头白猪的NOR众数为2,平均数为2.25;14头白猪的NOR众数为3,平均数为2.86;3头花猪的NOR众数分别为4.3、3,平均数为3.65、3.00和3.08。根据长白、枫泾和长白×枫泾杂一代和杂二代的NOR数目的区别和变化,NOR的遗传符合孟德尔定律。根据NOR数目与毛色的高度相关,提出了决定猪的黑白毛色的基因位于8号染色体并与NOR连锁的假设。猪的毛色除由位于8号染色体上的毛色基因所决定外,还应受其它基因位点的影响。 相似文献
149.
Mutations in the trfA replication gene of the broad-host-range plasmid RK2 result in elevated plasmid copy numbers. 总被引:10,自引:6,他引:4 下载免费PDF全文
Mutated forms of trfA, the replication protein gene of plasmid RK2, that support a minimal RK2 origin plasmid in Escherichia coli at copy numbers up to 23-fold higher than normal have been isolated. Six such high-copy-number (copy-up) mutations were mapped and sequenced. In each case, a single base transition led to an amino acid substitution in the TrfA protein primary sequence. The six mutations affected different residues of the protein and were located within a 69-base-pair region encoding 24 amino acids. Dominance tests showed that each of the mutants can be suppressed by wild-type trfA in trans, but suppression is highly dependent on the amount of wild-type protein produced. Excess mutant TrfA protein provided in trans significantly increased the copy number of RK2 and other self-replicating derivatives of RK2 that contain a wild-type trfA gene. These observations suggest that the mutations affect a regulatory activity of the TrfA replication protein that is a key factor in the control of initiation of RK2 replication. 相似文献
150.
在小兴安岭原始林内和采伐迹地上,发现以下几种球蚜:落叶松球蚜(Adelges laricisVall.),落叶松绿球蚜(Sacchiphanles viridis Ratz.)、落叶松红瘿球蚜(S. rosegallis Liet Tsai)、冷杉球蚜(Aphrastasia pectinatae Chol.) 以及红松球蚜(Pineus cembraepinilcoreanus Zhang et Fang),它们在林内分布和大量发生,其原因除了针叶树种类和树木 相似文献