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61.
The herpes simplex virus 1 capsid is formed in the infected cell nucleus by way of a spherical, less robust intermediate called the procapsid. Procapsid assembly requires the capsid shell proteins (VP5, VP19C, and VP23) plus the scaffolding protein, pre-VP22a, a major component of the procapsid that is not present in the mature virion. Pre-VP22a is lost as DNA is packaged and the procapsid is transformed into the mature, icosahedral capsid. We have employed a cell-free assembly system to examine the role of the scaffolding protein in procapsid formation. While other reaction components (VP5, VP19C, and VP23) were held constant, the pre-VP22a concentration was varied, and the resulting procapsids were analyzed by electron microscopy and SDS-polyacrylamide gel electrophoresis. The results demonstrated that while standard-sized (T = 16) procapsids with a measured diameter of approximately 100 nm were formed above a threshold pre-VP22a concentration, at lower concentrations procapsids were smaller. The measured diameter was approximately 78 nm and the predicted triangulation number was 9. No procapsids larger than the standard size or smaller than 78-nm procapsids were observed in appreciable numbers at any pre-VP22a concentration tested. SDS-polyacrylamide gel analyses indicated that small procapsids contained a reduced amount of scaffolding protein compared to the standard 100-nm form. The observations indicate that the scaffolding protein concentration affects the structure of nascent procapsids with a minimum amount required for assembly of procapsids with the standard radius of curvature and scaffolding protein content. 相似文献
62.
Yamamoto Daigo Yamada Masanori Okugawa Homa Tanaka Kanji 《World journal of surgical oncology》2004,2(1):1-4
Background
Malignant pleural mesothelioma is a rare malignancy. The outcome remains poor despite complete surgical resection.Patients and methods
Eleven patients with histologicaly proven epithelial type malignant pleural mesothelioma undergoing extrapleural pneumonectomy with systemic chemotherapy and/or radiotherapy before and after surgical resection were retrospectively reviewed.Results
Ten out of 11 patients underwent complete surgical resection, of these 7 patients had stage I disease. Of these 7 patients, 5 are alive without any recurrence, a 2-year survival rate of 80% was observed in this group. There was no operative mortality or morbidity.Conclusion
Extrapleural pneumonectomy with perioperative adjuvant treatment is safe and effective procedure for epithelial type malignant pleural mesothelioma. 相似文献63.
PIA and rSesC Mixture Arisen Antibodies Could Inhibit the Biofilm-Formation in Staphylococcus aureus
Bahman Mirzaei Ryhane Babaei Mohammad Reza Haghshenas Fatemeh Mohammadi Pegah Homayoni Ebrahim Shafaei 《Reports of Biochemistry & Molecular Biology》2021,10(1):1
Background:Staphylococcus aureus as a causative agent of hospital-acquired infections has been considered as the primary concern in biomaterial-related infections (BAIs).Methods:Following the purification of polysaccharide intercellular adhesion (PIA) as an efficient macromolecule in biofilm formation in the native condition, recombinant S. epidermidis surface-exposed rSesC protein, with the most homology to clumping factor A (ClfA) in S. aureus was cloned and expressed in a prokaryotic host as well. Fourier transform infrared spectrometry (FTIR) and Western blotting procedure analyzed purified PIA and protein, respectively. Then, the immune response was evaluated by measuring total IgG titers. Moreover, the capacity of Anti-biofilm forming activity of arisen antibodies to a biofilm-forming S. aureus strains was assessed by the semi-quantitative micro-plate procedure.Results:Data showed that the total IgGs were boosted in mice immunized sera. By performing an inhibition assay, the biofilm inhibitory effect of secreted antibodies to test strain was observed. Arisen antibodies against the mixture significantly were more potent than PIA and rSesC, when comparing individual antigens in a biofilm inhibition assay.Conclusion:immunization of mice with mentioned antigens especially a mixture of them, could eliminate the biofilm formation process in S. aureus. Hopefully, this study corresponds to the suggestion that the immunization of mice with PIA and rSesC candidate vaccines could protect against S. aureus infection.Key Words: PIA, Purification, Staphylococcus aureus, rSesC, Vaccine candidates 相似文献
64.
The propagation of surface plasmon polariton at an interface of metallic thin film and chiral sculptured thin film theoretically has been investigated using the transfer matrix method in the Kretschman configuration. The optical absorption of structure as a function of polar incident angle for linear polarization P and S has been calculated at different structural parameters. The results show that exist multiple plasmon peaks for P polarization, while there are the weak plasmon peaks when incident of light is S-polarized plane wave. 相似文献
65.
Mehdipoor Mohammad Damirchi Arsalan Razavi Tousi Seyed Mohammad Taghi Babaei Parvin 《Journal of physiology and biochemistry》2021,77(1):75-84
Journal of Physiology and Biochemistry - Although the role of vitamin D in various types of disorders such as cancer and diabetes has been well recognized, its relation to cardiovascular diseases... 相似文献
66.
Mehdipoor Mohammad Damirchi Arsalan Tousi Seyed Mohammad Taghi Razavi Babaei Parvin 《Journal of physiology and biochemistry》2021,77(2):341-341
Journal of Physiology and Biochemistry - A Correction to this paper has been published: https://doi.org/10.1007/s13105-021-00795-z 相似文献
67.
Homayoon Bahrami Mansour Zahedi Ali Akbar Moosavi-Movahedi Homa Azizian Massoud Amanlou 《Cell biochemistry and biophysics》2011,59(2):79-88
The nature of protein–sorbitol–water interaction in solution at the molecular level, has been investigated using molecular
dynamics simulations. In order to do this task, two molecular dynamics simulations of the protein ADH in solution at room
temperature have been carried out, one in the presence (about 0.9 M) and another in the absence of sorbitol. The results show
that the sorbitol molecules cluster and move toward the protein, and form hydrogen bonds with protein. Also, coating by sorbitol
reduces the conformational fluctuations of the protein compared to the sorbitol-free system. Thus, it is concluded that at
moderate concentration of sorbitol solution, sorbitol molecules interact with ADH via many H-bonds that prevent the protein
folding. In fact, at more concentrated sorbitol solution, water and sorbitol molecules accumulate around the protein surface
and form a continuous space-filling network to reduce the protein flexibility. Namely, in such solution, sorbitol molecules
can stabilize a misfolded state of ADH, and prevent the protein from folding to its native structure. 相似文献
68.
Negar Babaei Omali Zhenjun Zhao Ling Zhong Mark J. Raftery Hua Zhu Jerome Ozkan 《Biofouling》2013,29(7):697-709
This study was designed to use multiple reaction monitoring (MRM) for accurate quantification of contact lens protein deposits. Worn lenses used with a multipurpose disinfecting solution were collected after wear. Individual contact lenses were extracted and then digested with trypsin. MRM in conjunction with stable-isotope-labeled peptide standards was used for protein quantification. The results show that lysozyme was the major protein detected from both lens types. The amount of protein extracted from contact lenses was affected by the lens material. Except for keratin-1 (0.83 ± 0.61 vs 0.77 ± 0.20, p = 0.81) or proline rich protein-4 (0.11 ± 0.04 vs 0.15 ± 0.12, p = 0.97), the amounts of lysozyme, lactoferrin, or lipocalin-1 extracted from balafilcon A lenses (12.9 ± 9.01, 0.84 ± 0.50 or 2.06 ± 1.6, respectively) were significantly higher than that extracted from senofilcon A lenses (0.88 ± 0.13, 0.50 ± 0.10 or 0.27 ± 0.23, respectively) (p < 0.05). The amount of protein extracted from contact lenses was dependent on both the individual wearer and the contact lens material. This may have implications for the development of clinical responses during lens wear for different people and with different types of contact lenses. The use of MRM-MS is a powerful analytical tool for the quantification of specific proteins from single contact lenses after wear. 相似文献
69.
70.
Razieh Salehi Ashani Homa Azizian Nahid Sadeghi Alavijeh Vaezeh Fathi Vavsari Shabnam Mahernia Niloofar Sheysi Mahmood Biglar Massoud Amanlou Saeed Balalaie 《化学与生物多样性》2020,17(5)
A series of new deferasirox derivatives were synthesized through the reaction of monosubstituted hydrazides with 2‐(2‐hydroxyphenyl)‐4H‐benzo[e][1,3]oxazin‐4‐one. For the first time, deferasirox and some of its derivatives were evaluated for their in vitro inhibitory activity against Jack bean urease. The potencies of the members of this class of compounds are higher than that of acetohydroxamic acid. Two compounds, bearing tetrazole and hydrazine derivatives (bioisoester of carboxylate group), represented the most potent urease inhibitory activity with IC50 values of 1.268 and 3.254 μm , respectively. In silico docking studies were performed to delineate possible binding modes of the compounds with the enzyme, urease. Docking analysis suggests that the synthesized compounds were anchored well in the catalytic site and extending to the entrance of binding pocket and thus restrict the mobility of the flap by interacting with its crucial amino acid residues, CME592 and His593. The overall results of urease inhibition have shown that these target compounds can be further optimized and developed as a lead skeleton for the discovery of novel urease inhibitors 相似文献