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排序方式: 共有158条查询结果,搜索用时 31 毫秒
61.
Long-Range 1H-15N Heteronuclear Shift Correlation at Natural Abundance: a Tool To Study Benzothiazole Biodegradation by Two Rhodococcus Strains
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Pascale Besse Bruno Combourieu Gaëlle Boyse Martine Sancelme Heleen De Wever Anne-Marie Delort 《Applied microbiology》2001,67(4):1412-1417
The biodegradation of benzothiazole and 2-hydroxybenzothiazole by two strains of Rhodococcus was monitored by reversed phase high-pressure liquid chromatography and by 1H nuclear magnetic resonance (NMR). Both xenobiotics were biotransformed into a hydroxylated derivative of 2-hydroxybenzothiazole by these two strains. The chemical structure of this metabolite was determined by a new NMR methodology: long-range 1H-15N heteronuclear shift correlation without any previous 15N enrichment of the compound. This powerful NMR tool allowed us to assign the metabolite structure to 2,6-dihydroxybenzothiazole. 相似文献
62.
Malika Chegary Heleen te Brinke Jos P.N. Ruiter Frits A. Wijburg Maria S.K. Stoll Paul E. Minkler Michel van Weeghel Horst Schulz Charles L. Hoppel Ronald J.A. Wanders Sander M. Houten 《Biochimica et Biophysica Acta (BBA)/Molecular and Cell Biology of Lipids》2009,1791(8):806-815
Several mouse models for mitochondrial fatty acid β-oxidation (FAO) defects have been developed. So far, these models have contributed little to our current understanding of the pathophysiology. The objective of this study was to explore differences between murine and human FAO. Using a combination of analytical, biochemical and molecular methods, we compared fibroblasts of long chain acyl-CoA dehydrogenase knockout (LCAD−/−), very long chain acyl-CoA dehydrogenase knockout (VLCAD−/−) and wild type mice with fibroblasts of VLCAD-deficient patients and human controls. We show that in mice, LCAD and VLCAD have overlapping and distinct roles in FAO. The absence of VLCAD is apparently fully compensated, whereas LCAD deficiency is not. LCAD plays an essential role in the oxidation of unsaturated fatty acids such as oleic acid, but seems redundant in the oxidation of saturated fatty acids. In strong contrast, LCAD is neither detectable at the mRNA level nor at the protein level in men, making VLCAD indispensable in FAO. Our findings open new avenues to employ the existing mouse models to study the pathophysiology of human FAO defects. 相似文献
63.
K. Serge Bobo Matthias Waltert N. Moses Sainge John Njokagbor Heleen Fermon Michael Mühlenberg 《Biodiversity and Conservation》2006,15(13):4097-4117
Vegetation surveys were carried out at 24 sampling stations distributed over four land use types, namely near-primary forest,
secondary forest, agroforestry systems and annual crop lands in the northeastern part of the Korup region, Cameroon, to assess
the impact of forest conversion on trees and understorey plants. Tree species richness decreased significantly with increasing
level of habitat modification, being highest and almost equal in secondary and near-primary forests. Understorey plant species
richness was significantly higher in annual crop lands than in other land use types. The four land use types differed in tree
and understorey plant species composition, the difference being smaller among natural forests. Tree and understorey plant
density differed significantly between habitat types. Density was strongly correlated with species richness, both for trees
and understorey plants. Five tree and 15 understorey plant species showed significant responses to habitat. A 90% average
drop in tree basal area from forest to farmland was registered. Our findings support the view that agroforestry systems with
natural shade trees can serve to protect many forest species, but that especially annual crop lands could be redesigned to
improve biodiversity conservation in agricultural landscapes of tropical rainforest regions. 相似文献
64.
In situ localization of transketolase activity in epithelial cells of different rat tissues and subcellularly in liver parenchymal cells. 总被引:1,自引:0,他引:1
Joan Boren Antonio Ramos-Montoya Klazien S Bosch Heleen Vreeling Ard Jonker Josep J Centelles Marta Cascante Wilma M Frederiks 《The journal of histochemistry and cytochemistry》2006,54(2):191-199
Metabolic mapping of enzyme activities (enzyme histochemistry) is an important tool to understand (patho)physiological functions of enzymes. A new enzyme histochemical method has been developed to detect transketolase activity in situ in various rat tissues and its ultrastructural localization in individual cells. In situ detection of transketolase is important because this multifunctional enzyme has been related with diseases such as cancer, diabetes, Alzheimer's disease, and Wernicke-Korsakoff's syndrome. The proposed method is based on the tetrazolium salt method applied to unfixed cryostat sections in the presence of polyvinyl alcohol. The method appeared to be specific for transketolase activity when the proper control reaction is performed and showed a linear increase of the amount of final reaction product with incubation time. Transketolase activity was studied in liver, small intestine, trachea, tongue, kidney, adrenal gland, and eye. Activity was found in liver parenchyma, epithelium of small intestine, trachea, tongue, proximal tubules of kidney and cornea, and ganglion cells in medulla of adrenal gland. To demonstrate transketolase activity ultrastructurally in liver parenchymal cells, the cupper iron method was used. It was shown that transketolase activity was present in peroxisomes and at membranes of granular endoplasmic reticulum. This ultrastructural localization is similar to that of glucose-6-phosphate dehydrogenase activity, suggesting activity of the pentose phosphate pathway at these sites. It is concluded that the method developed for in situ localization of transketolase activity for light and electron microscopy is specific and allows further investigation of the role of transketolase in (proliferation of) cancer cells and other pathophysiological processes. 相似文献
65.
Perrault I Saunier S Hanein S Filhol E Bizet AA Collins F Salih MA Gerber S Delphin N Bigot K Orssaud C Silva E Baudouin V Oud MM Shannon N Le Merrer M Roche O Pietrement C Goumid J Baumann C Bole-Feysot C Nitschke P Zahrate M Beales P Arts HH Munnich A Kaplan J Antignac C Cormier-Daire V Rozet JM 《American journal of human genetics》2012,90(5):864-870
Mainzer-Saldino syndrome (MSS) is a rare disorder characterized by phalangeal cone-shaped epiphyses, chronic renal failure, and early-onset, severe retinal dystrophy. Through a combination of ciliome resequencing and Sanger sequencing, we identified IFT140 mutations in six MSS families and in a family with the clinically overlapping Jeune syndrome. IFT140 is one of the six currently known components of the intraflagellar transport complex A (IFT-A) that regulates retrograde protein transport in ciliated cells. Ciliary abundance and localization of anterograde IFTs were altered in fibroblasts of affected individuals, a result that supports the pivotal role of IFT140 in proper development and function of ciliated cells. 相似文献
66.
Heleen Rienstra Kirankumar Katta Johanna W. A. M. Celie Harry van Goor Gerjan Navis Jacob van den Born Jan-Luuk Hillebrands 《PloS one》2010,5(2)
Background
Chronic transplant dysfunction explains the majority of late renal allograft loss and is accompanied by extensive tissue remodeling leading to transplant vasculopathy, glomerulosclerosis and interstitial fibrosis. Matrix proteoglycans mediate cell-cell and cell-matrix interactions and play key roles in tissue remodeling. The aim of this study was to characterize differential heparan sulfate proteoglycan and chondroitin sulfate proteoglycan expression in transplant vasculopathy, glomerulosclerosis and interstitial fibrosis in renal allografts with chronic transplant dysfunction.Methods
Renal allografts were transplanted in the Dark Agouti-to-Wistar Furth rat strain combination. Dark Agouti-to-Dark Agouti isografts and non-transplanted Dark Agouti kidneys served as controls. Allograft and isograft recipients were sacrificed 66 and 81 days (mean) after transplantation, respectively. Heparan sulfate proteoglycan (collXVIII, perlecan and agrin) and chondroitin sulfate proteoglycan (versican) expression, as well as CD31 and LYVE-1 (vascular and lymphatic endothelium, respectively) expression were (semi-) quantitatively analyzed using immunofluorescence.Findings
Arteries with transplant vasculopathy and sclerotic glomeruli in allografts displayed pronounced neo-expression of collXVIII and perlecan. In contrast, in interstitial fibrosis expression of the chondroitin sulfate proteoglycan versican dominated. In the cortical tubular basement membranes in both iso- and allografts, induction of collXVIII was detected. Allografts presented extensive lymphangiogenesis (p<0.01 compared to isografts and non-transplanted controls), which was associated with induced perlecan expression underneath the lymphatic endothelium (p<0.05 and p<0.01 compared to isografts and non-transplanted controls, respectively). Both the magnitude of lymphangiogenesis and perlecan expression correlated with severity of interstitial fibrosis and impaired graft function.Interpretation
Our results reveal that changes in the extent of expression and the type of proteoglycans being expressed are tightly associated with tissue remodeling after renal transplantation. Therefore, proteoglycans might be potential targets for clinical intervention in renal chronic transplant dysfunction. 相似文献67.
Ecaterina Vasluian Ingrid G. M. de Jong Wim G. M. Janssen Margriet J. Poelma Iris van Wijk Heleen A. Reinders-Messelink Corry K. van der Sluis 《PloS one》2013,8(6)
Background
Youngsters with unilateral congenital below-elbow deficiency (UCBED) seem to function well with or without a prosthesis. Reasons for rejecting prostheses have been reported earlier, but unfortunately not those of the children themselves. Furthermore, reasons for acceptance are underexplored in the literature.Objectives
To investigate opinions of children and early and late adolescents with UCBED, and those of their parents and healthcare professionals, concerning (1) reasons to wear or not to wear prostheses and (2) about rehabilitation care.Methods
During one week of online focus group interviews, 42 children of 8–12 y/o, early and late adolescents of 13–16 and 17–20 y/o, 17 parents, and 19 healthcare professionals provided their opinions on various topics. This study addresses prosthetic use or non-use of prosthetics and rehabilitation care. Data were analyzed using the framework approach.Results
Cosmesis was considered to be the prime factor for choosing and wearing a prosthesis, since this was deemed especially useful in avoiding stares from others. Although participants functioned well without prostheses, they agreed that it was an adjuvant in daily-life activities and sports. Weight and limited functionality constituted rejection reasons for a prosthesis. Children and adolescents who had accepted that they were different no longer needed the prosthesis to avoid being stared at. The majority of participants highly valued the peer-to-peer contact provided by the healthcare professionals.Conclusions
For children and adolescents with UCBED, prostheses appeared particularly important for social integration, but much less so for functionality. Peer-to-peer contact seemed to provide support during the process of achieving social integration and should be embedded in the healthcare process. 相似文献68.
Bredrup C Saunier S Oud MM Fiskerstrand T Hoischen A Brackman D Leh SM Midtbø M Filhol E Bole-Feysot C Nitschké P Gilissen C Haugen OH Sanders JS Stolte-Dijkstra I Mans DA Steenbergen EJ Hamel BC Matignon M Pfundt R Jeanpierre C Boman H Rødahl E Veltman JA Knappskog PM Knoers NV Roepman R Arts HH 《American journal of human genetics》2011,(5):634-643
A subset of ciliopathies, including Sensenbrenner, Jeune, and short-rib polydactyly syndromes are characterized by skeletal anomalies accompanied by multiorgan defects such as chronic renal failure and retinitis pigmentosa. Through exome sequencing we identified compound heterozygous mutations in WDR19 in a Norwegian family with Sensenbrenner syndrome. In a Dutch family with the clinically overlapping Jeune syndrome, a homozygous missense mutation in the same gene was found. Both families displayed a nephronophthisis-like nephropathy. Independently, we also identified compound heterozygous WDR19 mutations by exome sequencing in a Moroccan family with isolated nephronophthisis. WDR19 encodes IFT144, a member of the intraflagellar transport (IFT) complex A that drives retrograde ciliary transport. We show that IFT144 is absent from the cilia of fibroblasts from one of the Sensenbrenner patients and that ciliary abundance and morphology is perturbed, demonstrating the ciliary pathogenesis. Our results suggest that isolated nephronophthisis, Jeune, and Sensenbrenner syndromes are clinically overlapping disorders that can result from a similar molecular cause. 相似文献
69.
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