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31.
Iwao Koyama Haruo Yamagami Toyoyasu Kuwae Munetsugu Kurata 《Prostaglandins & other lipid mediators》1982,23(6):777-785
The release of 6-keto-prostaglandin F1α (6KF1α)_and of thromboxane B2 (TXB2) from cells were investigated using mouse peritoneal exudate cells (PECs) and non-cultured peritoneal macrophages. They were prepared by adhesion to glass dishes and incubated for 1 hr at 37°C in 5% Co2 in air. Both the percentage of spreading macrophages and the release of 6KF1α and TXb2 increased in proportion to the incubation time. 6KF1α and TXB2 were released from the macrophages, not from the non-adherent cells. When PECs were incubated in silicon-coated glass dishes, the spreading of macrophages was hardly detected and lower amounts of 6KF1α and TXB2 were released from these cells compared with cells incubated in non-treated glass dishes. These findings suggest that adhesion with the correlated spreading of macrophages on glass dishes serve as a considerable physical factor for the release of 6KF1α and TXB2. 相似文献
32.
Maung Maung Theint San Thwe Thidalay Myat Myat Zaw Khin Shimada Tomofumi Bawm Saw Kobayashi Motoko Saing Khin Maung Katakura Ken Arai Satoru Suzuki Hitoshi 《Journal of Mammalian Evolution》2021,28(2):497-509
Journal of Mammalian Evolution - We addressed the spatiotemporal characteristics of four commensal rodent species occurring in Myanmar in comparison with other areas of the Indo-Malayan region. We... 相似文献
33.
Haruo Ogura Hiroshi Takahashi Kikuko Ohokubo 《Nucleosides, nucleotides & nucleic acids》2013,32(2):147-154
Abstract The reaction of glycosyl isothiocyanates (la, b, c, d, e) with 5,6-diamino-1-3-dimethyluracil gave the respective 1-glycosyl-3-(6-amino-1, 3-dimethyl-2, 4-dioxopyrimidine-5-yl) thioureas (2a, b, c, d, e) in excellent yields. Treatment of these thioureas with NBS afforded the respective 5,7-dioxopyrimido-[5,4-e]-as-triazine glycosides (4a, b, c, d, e) in good yields. 相似文献
34.
Seiji Hayashizaki Shinobu Hirai Yumi Ito Yoshiko Honda Yosefu Arime Ichiro Sora Haruo Okado Tohru Kodama Masahiko Takada 《PloS one》2013,8(10)
Dopamine regulates the psychomotor stimulant activities of amphetamine-like substances in the brain. The effects of dopamine are mediated through five known dopamine receptor subtypes in mammals. The functional relevance of D5 dopamine receptors in the central nervous system is not well understood. To determine the functional relevance of D5 dopamine receptors, we created D5 dopamine receptor-deficient mice and then used these mice to assess the roles of D5 dopamine receptors in the behavioral response to methamphetamine. Interestingly, D5 dopamine receptor-deficient mice displayed increased ambulation in response to methamphetamine. Furthermore, dopamine transporter threonine phosphorylation levels, which regulate amphetamine-induced dopamine release, were elevated in D5 dopamine receptor-deficient mice. The increase in methamphetamine-induced locomotor activity was eliminated by pretreatment with the dopamine transporter blocker GBR12909. Taken together, these results suggest that dopamine transporter activity and threonine phosphorylation levels are regulated by D5 dopamine receptors. 相似文献
35.
Tomohiro Nishimura Shinya Sato Wittaya Tawong Hiroshi Sakanari Keita Uehara Md Mahfuzur Rahman Shah Shoichiro Suda Takeshi Yasumoto Yohsuke Taira Haruo Yamaguchi Masao Adachi 《PloS one》2013,8(4)
Background
The marine epiphytic dinoflagellate genus Gambierdiscus produce toxins that cause ciguatera fish poisoning (CFP): one of the most significant seafood-borne illnesses associated with fish consumption worldwide. So far, occurrences of CFP incidents in Japan have been mainly reported in subtropical areas. A previous phylogeographic study of Japanese Gambierdiscus revealed the existence of two distinct phylotypes: Gambierdiscus sp. type 1 from subtropical and Gambierdiscus sp. type 2 from temperate areas. However, details of the genetic diversity and distribution for Japanese Gambierdiscus are still unclear, because a comprehensive investigation has not been conducted yet.Methods/Principal Finding
A total of 248 strains were examined from samples mainly collected from western and southern coastal areas of Japan during 2006–2011. The SSU rDNA, the LSU rDNA D8–D10 and the ITS region were selected as genetic markers and phylogenetic analyses were conducted. The genetic diversity of Japanese Gambierdiscus was high since five species/phylotypes were detected: including two reported phylotypes (Gambierdiscus sp. type 1 and Gambierdiscus sp. type 2), two species of Gambierdiscus (G. australes and G. cf. yasumotoi) and a hitherto unreported phylotype Gambierdiscus sp. type 3. The distributions of type 3 and G. cf. yasumotoi were restricted to the temperate and the subtropical area, respectively. On the other hand, type 1, type 2 and G. australes occurred from the subtropical to the temperate area, with a tendency that type 1 and G. australes were dominant in the subtropical area, whereas type 2 was dominant in the temperate area. By using mouse bioassay, type 1, type 3 and G. australes exhibited mouse toxicities.Conclusions/Significance
This study revealed a surprising diversity of Japanese Gambierdiscus and the distribution of five species/phylotypes displayed clear geographical patterns in Japanese coastal areas. The SSU rDNA and the LSU rDNA D8–D10 as genetic markers are recommended for further use. 相似文献36.
Nao Kusuhashi Yukiyasu Tsutsumi Haruo Saegusa Kenji Horie Tadahiro Ikeda Kazumi Yokoyama Kazuyuki Shiraishi 《Proceedings. Biological sciences / The Royal Society》2013,280(1759)
We here describe a new Early Cretaceous (early Albian) eutherian mammal, Sasayamamylos kawaii gen. et sp. nov., from the ‘Lower Formation’ of the Sasayama Group, Hyogo Prefecture, Japan. Sasayamamylos kawaii is characterized by a robust dentary, a distinct angle on the ventral margin of the dentary at the posterior end of the mandibular symphysis, a lower dental formula of 3–4 : 1 : 4 : 3, a robust lower canine, a non-molariform lower ultimate premolar, and a secondarily reduced entoconid on the molars. To date, S. kawaii is the earliest known eutherian mammal possessing only four premolars, which demonstrates that the reduction in the premolar count in eutherians started in the late Early Cretaceous. The occurrence of S. kawaii implies that the relatively rapid diversification of eutherians in the mid-Cretaceous had already started by the early Albian. 相似文献
37.
Yoshitaka Kokusho Haruo Machida Shinjiro Iwasaki 《Bioscience, biotechnology, and biochemistry》2013,77(7):1743-1750
For the production of extracellular lipase by Alcaligenes species No. 679, NaNO3, polyoxyethylene alkyl ether, Fe++, sodium citrate and fructose were found to be effective. The enzyme was prepared by acetone precipitation from the filtrate of the culture broth of this strain. The enzyme was most active at pH 9.0 and 50°C, while 35% of its activity was lost on heat treatment at 60°C for 10 min. Sodium salts of bile acids stimulated the enzyme activity. This lipase could hydrolyse natural fats and oils as well as olive oil. During the hydrolysis of olive oil, monoglyceride was found to accumulate up to 70 mol percent. This lipase possesses special properties similar to those of pancreatic lipase as shown in the comparative experiments. 相似文献
38.
A simple and sensitive spectrophotometric method for determining meso-α,ε-diaminopime-late with meso-α,ε-diaminopimelate d-dehydrogenase (EC class 1.4.1) is described. meso-α,ε-Diaminopimelate was determined spectrophotometrically with the enzyme by measuring the NADPH formed (Procedure A) or the formazan produced by NADPH (Procedure B). A linear relationship was established between absorbance and the amount of amino acid (0.02-0.20 μmol). This method can be used to assay diaminopimelate epimerase (EC 5.1.1.7) and is applicable for determining meso-α,ε-diaminopimelate specifically in hydrolyzates of bacterial cell wall. 相似文献
39.
Kiyoshi Miwa Hiroshi Matsui Mahito Terabe Shigeru Nakamori Konosuke Sano Haruo Momose 《Bioscience, biotechnology, and biochemistry》2013,77(11):2901-2903
Seven filamentous (fil) mutants were isolated from B. subtilis, and the mutations were mapped by means of lysed-protoplast transformation. Five of the mutations were linked to aroD and the others to pyrD. rgn mutations, which lead to a decrease in autolysin(s) and the formation of filaments, were also linked to aroD, and the mapping order was rgn-dnaE-aroD. On comparison with other reported filamentous mutations (lyt-1, lyt-2 and lyt-152), fil-1, fil-3 to -6, rgn and the above lyt mutations were determined to be in the same locus. All of the seven fil strains lacked flagella and showed decreased aμtolysin activity. Among them, only mutants having arod- linked mutations showed low competency. Protease assay results indicated that rgn mutants produce a several times higher amount of the enzyme than the parent strain, and the initiation time for the production in rgn mutants was two hours earlier than in the parent strain. 相似文献
40.
Teruaki Shiroza Kazuo Furihata Toyoshige Endō Haruo Seto Noboru Ōtake 《Bioscience, biotechnology, and biochemistry》2013,77(5):1425-1427
Phosphatidylethanolamine N-methyltransferase, which catalyzes all the three-step methylation from phosphatidylethanolamine to phosphatidylcholine, was purified to homogeneity from the membrane fraction of Zymomonas mobilis. The purified enzyme exhibited a single band on SDS- polyacrylamide gel electrophoresis and its molecular weight was estimated to be 42,000 on comparison with those of marker proteins. The three activities dependent on phosphatidylethanolamine, phosphatidyl-N monomethylethanolamine and phosphatidyl-N Af-dimethylethanolamine of the purified enzyme showed similar pH profiles with an optimum of pH 8.5, and were enhanced in the same manner by Triton X-100 and l-cysteine. The maximal velocities of the three reactions for S-adenosyl-l-methionine were 0.04, 1.36 and 0.69 nmol/mg protein/min with apparent Michaelis constant values of 3.6, 1.9 and 3.9 fiM, respectively, indicating that the first-step methylation is rate-limiting for the pathway in the organism. 相似文献