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641.
642.
Herpes simplex virus VP16 rescues viral mRNA from destruction by the virion host shutoff function. 总被引:17,自引:0,他引:17
Q Lam C A Smibert K E Koop C Lavery J P Capone S P Weinheimer J R Smiley 《The EMBO journal》1996,15(10):2575-2581
643.
A chromosomal centric fusion polymorphism in populations of the plains pocket gopher, Geomys bursarius, was studied to determine the relative fitness associated with the karyotypic phenotypes. There was a greater number of heterozygous individuals than expected χ1 2=8.58, P=0.001. Calculations indicate that the viabilities of the two chromosomal homozygotes were only 35 and 76 percent or that of the heterozygote. Differences in fitness values for the chromosomal morphs for Geomys strongly emphasize the possible adaptive nature of the karyotype and provides a primary mechanism for chromosomal evolution, even in species composed of demes of relatively large size. This is the first case of positive chromosomal heterosis in vertebrates. The plains pocket gopher can now be added to the few empirically documented samples of balanced polymorphism. 相似文献
644.
Waltraud Kofer Christian Eibl Klaus Steinmüller Hans-Ulrich Koop 《In vitro cellular & developmental biology. Plant》1998,34(4):303-309
Summary Plastids are surrounded by an envelope consisting of a double membrane. This barrier has to be penetrated or overcome by the
DNA when transforming the plastome. Both the biolistic and polyethylene glycol-mediated transformation techniques accomplish
this task, albeit by different mechanisms. We were the first laboratory to successfully use the polyethylene glycol (PEG)-method
for plastid transformation, yet we use the particle gun when appropriate. In this report we compare the two methods and discuss
their shortcomings and advantages. Plastid transformations with various constructs, mainly using theaadA gene as a selective marker, were performed. We point to potential problems likely to be encountered during the transformation
and selection processes and offer possibilities for improvement. We give further examples of the successful application of
plastome transformation and show its merits in addressing biological questions concerning the elucidation of plastid sequences
of unknown function and the control of plastid gene expression.
Based on a presentation in the symposium “Organelle Transformation” during the 1997 SIVB Congress held in Washington, DC June
14–18, 1997.
An erratum to this article is available at . 相似文献
645.