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351.
Evidence for the association of villin with core filaments and rootlets of intestinal epithelial microvilli 总被引:7,自引:0,他引:7
Prof. Dr. Detlev Drenckhahn Hans-Dieter Hofmann Hans Georg Mannherz 《Cell and tissue research》1983,228(2):409-414
Villin, a 95,000 dalton polypeptide of intestinal brush border which is known to bundle or sever actin filaments in a Ca++-dependent manner, was localized in rat and chicken intestinal epithelium by means of immunocytochemistry at the light- and electron-microscopic levels. Specific antibodies to villin were raised in rabbits immunized with villin purified from chicken intestinal epithelium. Anti-villin bound selectively to the microvillus filament bundle from its tip down to the rootlets. These findings indicate that the well-known stability of rootlet filaments towards elevated Ca++ ion concentrations cannot be explained by the absence of villin. Therefore additional factors must exist which prevent the rootlets from Ca++-villin mediated disassembly. 相似文献
352.
353.
Benelli Jéssica Louise Basso Rossana Patrícia Grafulha Talita Werner Poester Vanice Rodrigues Munhoz Lívia Silveira Martins Katheryne Benine Zogbi Heruza Einsfeld Von Groll Andrea Severo Cecília Bittencourt Stevens David A. Xavier Melissa Orzechowski 《Mycopathologia》2022,187(4):397-404
Mycopathologia - Opportunistic infections are serious complications in critically ill COVID-19 patients, especially co-infections with bacterial and fungal agents. Here we report a rare case of... 相似文献
354.
Anti-idiotypic antibodies as probes of cell surface receptors 总被引:3,自引:0,他引:3
Glen N. Gaulton Man Sung Co Hans-Dieter Royer Mark I. Greene 《Molecular and cellular biochemistry》1984,65(1):5-21
Summary Anti-idiotypic antibodies have proven to have unique applications as probes in both functional and biochemical studies of cell surface receptors. Anti-idiotypic receptor antibodies have been prepared to antibodies which bind to purified ligand, as in the case of insulin, retinol-binding protein, the mammalian reovirus receptor, and the neutrophil chemotatic receptor, and to natural ligand analogs, such as the beta-adrenergic antagonist alprenolol. These systems have documented the usefulness of anti-idiotypic antibodies in the quantitation and modulation of specific membrane receptors on a variety of cell types. Anti-idotypic antibodies have also been utilized for the isolation of specific membrane receptors, e.g., reovirus and B-1 H globulin receptors. Some anti-idiotypic receptor antibodies, e.g., insulin and reovirus systems, have been shown to mimic the physiological properties of ligand upon binding to cellular receptors. These antibodies enable a new dimension of both receptor based cellular studies and therapeutic regimens. This review focuses on the past use of anti-idiotypic antibodies as probes of cell surface receptors, and on the methodologies required for the successful application of anti-idiotypic antibodies for use in further membrane receptor studies, and of the genes which encode and regulate these receptors. We also discuss the use of anti-idiotypic antibodies in the understanding of and therapeutic approach to receptor related diseases.Dr. Gaulton received the NRSA Viral Oncology Training Grant t# CA 09031. 相似文献
355.
Johanna Vappiani Tom Eyster Keegan Orzechowski Diana Ritz Pramthesh Patel Daniel Sévin Juan Aon 《Biotechnology progress》2021,37(6):e3198
Adjustments to CHO cell physiology were recently observed during implementation of a Raman spectroscopy-based glucose and lactate control strategy. To further understand how these cells, under monoclonal antibody (mAb) production conditions, utilized the extra lactic acid fed, we performed a comprehensive semi-quantitative and time-dependent analysis of the exometabolome. This study focused on the CHO cell's metabolic shift from the fifth day of culture. We compared relative levels of extracellular metabolites in the absence or presence of a 2 g/L lactic acid setpoint while glucose was kept at 4 g/L. Our hypothesis is that extra lactic acid would supply more pyruvate, favoring oxidative phosphorylation. We subsequentially uncovered several carnitine derivatives as biomarkers of the simultaneous activation of TCA anaplerotic pathways as well as a carbon-buffering pathway. CHO cells exhibited a balance between intermediates from (i) amino acid catabolism, (ii) fatty acid β-oxidation, and (iii) pyruvate from glycolysis and lactic acid; and the secretion of their intermediate carnitine derivatives. In addition, 3-hydroxy-methyl-glutaric acid (HMG) and mevalonate syntheses were found as biomarkers of alternative acyl group removal. Together, under a limited capacity to assimilate the surplus of acyl-CoA groups as well as an ability to maintain the acyl-CoA: free CoA ratio for proper and continuous functioning of the TCA cycle, CHO cells activate the carnitine-buffering system, HMG, and mevalonate pathways. 相似文献
356.
Sławomir Orzechowski Joanna Socha-Hanc Andrzej Paszkowski 《Acta Physiologiae Plantarum》1999,21(4):331-334
The intracellular distribution of alanine aminotransferase (AlaAT, EC 2.6.1.2) activity with L-alanine and 2-oxoglutarate
as a substrates in maize whole leaf extract and bundle sheath cells was studied. After isolation of the mitochondrial-peroxisomal
fraction, mitochondria and peroxisomes were separated by centrifugation on a linear 40–52 % (w/w) sucrose gradient. L-Alanine-2-oxoglutarate
transaminating activity of whole leaf extract showed two peaks: first distinctly higher associated with mitochondria and second
lower with peroxisomes. In bundle sheath cells only one peak of this activity was found. It corresponded to the mitochondrial
region of the gradient. It is proposed that mitochondrial L-alanine — 2-oxoglutarate activity was brought about by AlaAT.
Glycine aminotransferase (EC 2.6.1.4) could be responsible for the same activity in peroxisomes.
This work was supported by the State Committee for Scientific Research, a grant No. 5PO6A00510 相似文献