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排序方式: 共有225条查询结果,搜索用时 31 毫秒
41.
Anna Schorcht Christopher A. Cottrell Pavel Pugach Rajesh P. Ringe Alvin X. Han Joel D. Allen Tom L. G. M. van den Kerkhof Gemma E. Seabright Edith E. Schermer Thomas J. Ketas Judith A. Burger Jelle van Schooten Celia C. LaBranche Gabriel Ozorowski Natalia de Val Daniel L. V. Bader Hanneke Schuitemaker Colin A. Russell David C. Montefiori Marit J. van Gils Max Crispin P. J. Klasse Andrew B. Ward John P. Moore Rogier W. Sanders 《Journal of virology》2022,96(1)
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Octopine and nopaline strains of Agrobacterium tumefaciens differ in virulence; molecular characterization of the virF locus 总被引:11,自引:0,他引:11
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Dirkje W Sommeijer Aida Beganovic Casper G Schalkwijk Hanneke Ploegmakers Chris M van der Loos Benien E van Aken Hugo ten Cate Allard C van der Wal 《The journal of histochemistry and cytochemistry》2004,52(9):1141-1149
OBJECTIVE: One of the possible pathological mechanisms behind the increased vascular injury in diabetes mellitus type 2 (DM2) is the formation of advanced glycation end products (AGEs). The aim of this study was to investigate whether the presence of AGEs and specific markers for coagulation and inflammation in symptomatic atherosclerotic plaques from DM2 patients differs from plaques from nondiabetics. METHODS AND RESULTS: Carotid atherectomies were obtained from DM2 patients (n=11) and controls without DM2 matched for age and other cardiovascular risk factors (n=12) who were treated for symptomatic carotid artery stenosis. Plaques were graded according to the American Heart Association classification of lesions. More fibrosis and more thrombotic complications (p=0.007) were observed in carotid atherectomies from DM2 patients. Percentages of immunostained smooth muscle cells and macrophages in the lesions, quantified planimetrically, did not differ between the two groups. No differences were found in the immunostaining for T cells, tissue factor (TF), endothelial protein C receptor (EPCR), nuclear factor kappaB, and the AGE carboxymethyllysine. CONCLUSIONS: These findings demonstrate that DM2 is associated with increased plaque complications; however, a local changed presence of AGEs, TF, and EPCR seems not to be involved in this end stage of atherosclerosis. 相似文献
46.
Alison E. Mahan Madeleine F. Jennewein Todd Suscovich Kendall Dionne Jacquelynne Tedesco Amy W. Chung Hendrik Streeck Maria Pau Hanneke Schuitemaker Don Francis Patricia Fast Dagna Laufer Bruce D. Walker Lindsey Baden Dan H. Barouch Galit Alter 《PLoS pathogens》2016,12(3)
Antibody effector functions, such as antibody-dependent cellular cytotoxicity, complement deposition, and antibody-dependent phagocytosis, play a critical role in immunity against multiple pathogens, particularly in the absence of neutralizing activity. Two modifications to the IgG constant domain (Fc domain) regulate antibody functionality: changes in antibody subclass and changes in a single N-linked glycan located in the CH2 domain of the IgG Fc. Together, these modifications provide a specific set of instructions to the innate immune system to direct the elimination of antibody-bound antigens. While it is clear that subclass selection is actively regulated during the course of natural infection, it is unclear whether antibody glycosylation can be tuned, in a signal-specific or pathogen-specific manner. Here, we show that antibody glycosylation is determined in an antigen- and pathogen-specific manner during HIV infection. Moreover, while dramatic differences exist in bulk IgG glycosylation among individuals in distinct geographical locations, immunization is able to overcome these differences and elicit antigen-specific antibodies with similar antibody glycosylation patterns. Additionally, distinct vaccine regimens induced different antigen-specific IgG glycosylation profiles, suggesting that antibody glycosylation is not only programmable but can be manipulated via the delivery of distinct inflammatory signals during B cell priming. These data strongly suggest that the immune system naturally drives antibody glycosylation in an antigen-specific manner and highlights a promising means by which next-generation therapeutics and vaccines can harness the antiviral activity of the innate immune system via directed alterations in antibody glycosylation in vivo. 相似文献
47.
Euler Z van den Kerkhof TL van Gils MJ Burger JA Edo-Matas D Phung P Wrin T Schuitemaker H 《Journal of virology》2012,86(4):2045-2055
We previously established that at 3 years postseroconversion, ~30% of HIV-infected individuals have cross-reactive neutralizing activity (CrNA) in their sera. Here we studied the kinetics with which CrNA develops and how these relate to the development of autologous neutralizing activity as well as viral escape and diversification. For this purpose, sera from five individuals with CrNA and one elite neutralizer that were obtained at three monthly intervals in the first year after seroconversion and at multiple intervals over the disease course were tested for neutralizing activity against an established multiclade panel of six viruses. The same serum samples, as well as sera from three individuals who lacked CrNA, were tested for their neutralizing activities against autologous clonal HIV-1 variants from multiple time points covering the disease course from seroconversion onward. The elite neutralizer already had CrNA at 9.8 months postseroconversion, in contrast with the findings for the other five patients, in whom CrNA was first detected at 20 to 35 months postseroconversion and peaked around 35 months postseroconversion. In all patients, CrNA coincided with neutralizing activity against autologous viruses that were isolated <12 months postseroconversion, while viruses from later time points had already escaped autologous neutralizing activity. Also, the peak in gp160 sequence diversity coincided with the peak of CrNA titers. Individuals who lacked CrNA had lower peak autologous neutralizing titers, viral escape, and sequence diversity than individuals with CrNA. A better understanding of the underlying factors that determine the presence of CrNA or even an elite neutralizer phenotype may aid in the design of an HIV-1 vaccine. 相似文献
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Twan van den Beucken Marianne Koritzinsky Hanneke Niessen Ludwig Dubois Kim Savelkouls Hilda Mujcic Barry Jutten Juraj Kopacek Sylvia Pastorekova Albert J. van der Kogel Philippe Lambin Willem Voncken Kasper M. A. Rouschop Bradly G. Wouters 《The Journal of biological chemistry》2009,284(36):24204-24212
49.
Hanneke Korsten Angelique Ziel-van der Made Xiaoqian Ma Theo van der Kwast Jan Trapman 《PloS one》2009,4(5)
The PSA-Cre;Pten-loxP/loxP mouse prostate cancer model displays clearly defined stages of hyperplasia and cancer. Here, the initial stages of hyperplasia development are studied. Immunohistochemical staining showed that accumulated pAkt+ hyperplastic cells overexpress luminal epithelial cell marker CK8, and progenitor cell markers CK19 and Sca-1, but not basal epithelial cell markers. By expression profiling we identified novel hyperplastic cell markers, including Tacstd2 and Clu. Further we showed that at young age prostates of targeted Pten knockout mice contained in the luminal epithelial cell layer single pAkt+ cells, which overexpressed CK8, Sca-1, Tacstd2 and Clu; basal epithelial cells were always pAkt−. Importantly, in the luminal epithelial cell layer of normal prostates we detected rare Clu+Tacstd2+Sca-1+ progenitor cells. These novel cells are candidate tumor initiating cells in Pten knockout mice. Remarkably, all luminal epithelial cells in the proximal region of normal prostates were Clu+Tacstd2+Sca-1+. However, in PSA-Cre;Pten-loxP/loxP mice, the proximal prostate does not contain hyperplastic foci. Small hyperplastic foci in prostates of PSA-Cre;Pten-loxP/+ mice found at old age, showed complete Pten inactivation and a progenitor marker profile. Finally, we present a novel model of prostate development and renewal, including lineage-specific luminal epithelial progenitor cells. It is proposed that Pten deficiency induces a shift in the balance of differentiation to proliferation in these cells. 相似文献
50.
Eva Maria Feny? Alan Heath Stefania Dispinseri Harvey Holmes Paolo Lusso Susan Zolla-Pazner Helen Donners Leo Heyndrickx Jose Alcami Vera Bongertz Christian Jassoy Mauro Malnati David Montefiori Christiane Moog Lynn Morris Saladin Osmanov Victoria Polonis Quentin Sattentau Hanneke Schuitemaker Ruengpung Sutthent Terri Wrin Gabriella Scarlatti 《PloS one》2009,4(2)