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991.
992.
The alternative pathway in cucumber seedlings under low temperature stress was enhanced by salicylic acid 总被引:1,自引:0,他引:1
Tao Lei Hong Feng Xin Sun Qi-Lin Dai Fan Zhang Hou-Guo Liang Hong-Hui Lin 《Plant Growth Regulation》2010,60(1):35-42
The alternative pathway is a cyanide-resistant and non-phosphorylatory electron transport pathway in mitochondria of higher
plants. Alternative oxidase (AOX) is the terminal oxidase of this pathway. Our present study investigated the effect of exogenous
salicylic acid (SA) on alternative pathway in cucumber (Cucumis sativus L.) seedlings under low temperature stress. Results showed that during the process of low temperature stress, the alternative
pathway capacity was enhanced as AOX expression increased in SA pretreated seedlings. Compared with seedlings without SA pretreatment,
slower decrease of relative water content and lower levels of electrolyte leakage, H2O2 and malonyldialdehyde content were detected in SA pretreated seedlings. These results indicated that SA could alleviate the
injury caused by low temperature on cucumber seedlings. Since the special protective functions of alternative pathway and
AOX in plants, we suggested that the alternative pathway was related to SA-mediated plant resistance to environmental stresses
such as low temperature. 相似文献
993.
994.
Jianbo Xiao Tingting Chen Longsheng Chen Fan Yang 《Journal of inorganic biochemistry》2010,104(11):1148-201
Baicalein and genistein were studied for the affinities for human serum albumin (HSA) in the presence and absence of three CdTe quantum dots (QDs) with different sizes. Three typical CdTe QDs with maximum emissions of 535 nm (green-emitting, G-QDs), 598 nm (yellow-emitting, Y-QDs), and 654 nm (red-emitting, R-QDs) were tested. The fluorescence intensities of HSA decreased remarkably with increasing concentration of QDs. Baicalein resulted in an obvious blue-shift of the λem of HSA from 340 to 334 nm. However, the extents of blue-shifts induced by baicalein and genistein in the presence of QDs were much bigger than that in the absence of QDs. The quenching process of baicalein for HSA was easily affected by the QDs size than that of genistein. QDs increased the quenching constant from 136.97% to 162.24% for baicalein. However, QDs only increased the quenching constants from 20.56% to 32.23% for genistein. G-QDs, Y-QDs, and R-QDs increased the affinities of baicalein for HSA about 3.02%, 6.38% and 9.40%. G-QDs, Y-QDs, and R-QDs increased the affinities of genistein for HSA about 2.56%, 13.46% and 19.44%. The binding affinities of baicalein and genistein for HSA increased with increasing QDs size. 相似文献
995.
996.
Haiyan Wei Xuhua Mi Ling Ji Lichuan Yang Qingjie Xia Yuquan Wei Isamu Miyamori ChunYuan Fan 《Biochemistry. Biokhimii?a》2010,75(3):304-309
In this study, we focused on the relationship between aldosterone and NOX1 expression in vascular smooth muscle cells (VSMCs). For the first time, with the use of specific inhibitors of protein kinase
C (PKC), we report that PKCδ mediates upregulation of NOX1 induced by 10 nM aldosterone in cultured VSMCs. Participation of PKC in the mediation of NOX1 regulation was further confirmed by the effect of diacylglycerol, a PKC agonist, on the NOX1 RNA in A7r5 cells with Northern blot analysis. To establish cause and effect, we next silenced the PKCδ gene partly by RNA
interference and found knockdown of PKCδ gene attenuated aldosterone-induced NOX1 expression, generation of superoxide, as well as protein synthesis in VSMCs. Taken together, these data indicated PKCδ might
mediate aldosterone-dependent NOX1 upregulation in VSMCs. In addition, we showed that the cascade from aldosterone to PKCδ activation had the participation
of the mineralocorticoid receptor. 相似文献
997.
Mae Responte YiFan Chiu Po Peng Rafe M. Brown ChiaYen Dai YongChao Su 《Ecology and evolution》2021,11(16):11241
Oceanic islands are unique geographic systems that promote local adaptations and allopatric speciation in many of their highly endemic taxa. This is a common case in the Philippine Archipelago, where numerous unrelated taxa on islands have been inferred to have diversified in isolation. However, few cases have been reported in invertebrates especially among parasitic organisms. Here, we tested for biogeographical structure in novel populations of the “generalist" kleptoparasitic spider, Argyrodes lanyuensis Yoshida, Tso & Severinghaus, 1998 in the Philippines. Results showed that, in addition to Orchid/Lanyu Island, this species has a wide geographic distribution in the Philippine Archipelago. The estimated divergence time of this lineage using the mitochondrial cytochrome oxidase 1 (mt‐CO1) suggests that this species diverged ca 3.12 MYA, during the Pliocene. Two reciprocal monophyletic clades were elucidated in A. lanyuensis, but with limited differentiation across Pleistocene Aggregate Island Complex (PAIC) boundaries and modern‐day islands. However, in our analyses of morphological variation, we identified two phenotypically differentiated units in males (Orchid Island, Taiwan + Luzon, Philippine PAIC populations vs. Palawan + West Visayan + Mindanao PAIC populations). We infer that this species diverged in the southern portion of the Philippine Archipelago and only recently colonized Orchid Island. Our study provides new information on the extensive distribution of A. lanyuensis outside Orchid Island, Taiwan, but we documented a very limited geographically associated genetic variation. Our study points to behavioral phenomena such as foraging behavior as essential contributor to the evolutionary process of species diversification, in contrast to the traditionally invoked geographic drivers of divergence. 相似文献
998.
999.
六种检测猪瘟病毒方法的比较 总被引:1,自引:0,他引:1
【目的】本研究旨在比较6种检测猪瘟病毒方法的优缺点。【方法】应用病毒分离、胶体金免疫层析试纸条、抗原捕捉ELISA、反转录-聚合酶链式反应(RT-PCR)、TaqMan荧光定量RT-PCR(RT-qPCR)和反转录-环介导等温扩增方法(RT-LAMP)等6种方法,分别对50份疑似猪瘟病料中的猪瘟病毒(Classical swine fevervirus,CSFV)进行检测。【结果】结果表明:RT-qPCR和RT-LAMP方法检出阳性样品数为13份,RT-PCR为11份,病毒分离为10份,抗原捕捉ELISA为9份,胶体金试纸条为8份;6种方法均检测为阳性8份,均为阴性37份。【结论】结果提示,在对猪瘟病毒进行检测时,RT-qPCR、RT-LAMP和RT-PCR由于其灵敏性高,可作为首选检测方法,但操作时需要避免假阳性的出现;病毒分离方法虽然操作繁琐,但结果准确,是确诊猪瘟必不可少的检测方法;抗原捕捉ELISA和胶体金试纸条检测时间较短,由于其敏感性较低所限,主要用于对畜群进行检测,不适合个体检测。 相似文献
1000.