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131.
TJ Wardill PT Gonzalez-Bellido RJ Crook RT Hanlon 《Proceedings. Biological sciences / The Royal Society》2012,279(1745):4243-4252
Fast dynamic control of skin coloration is rare in the animal kingdom, whether it be pigmentary or structural. Iridescent structural coloration results when nanoscale structures disrupt incident light and selectively reflect specific colours. Unlike animals with fixed iridescent coloration (e.g. butterflies), squid iridophores (i.e. aggregations of iridescent cells in the skin) produce dynamically tuneable structural coloration, as exogenous application of acetylcholine (ACh) changes the colour and brightness output. Previous efforts to stimulate iridophores neurally or to identify the source of endogenous ACh were unsuccessful, leaving researchers to question the activation mechanism. We developed a novel neurophysiological preparation in the squid Doryteuthis pealeii and demonstrated that electrical stimulation of neurons in the skin shifts the spectral peak of the reflected light to shorter wavelengths (greater than 145 nm) and increases the peak reflectance (greater than 245%) of innervated iridophores. We show ACh is released within the iridophore layer and that extensive nerve branching is seen within the iridophore. The dynamic colour shift is significantly faster (17 s) than the peak reflectance increase (32 s), revealing two distinct mechanisms. Responses from a structurally altered preparation indicate that the reflectin protein condensation mechanism explains peak reflectance change, while an undiscovered mechanism causes the fast colour shift. 相似文献
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A large-scale comparison of computational models on the residue flexibility for NMR-derived proteins
As an alternative to X-ray crystallography, nuclear magnetic resonance (NMR) has also emerged as the method of choice for studying both protein structure and dynamics in solution. However, little work using computational models such as Gaussian network model (GNM) and machine learning approaches has focused on NMR-derived proteins to predict the residue flexibility, which is represented by the root mean square deviation (RMSD) with respect to the average structure. We provide a large-scale comparison of computational models, including GNM, parameter-free GNM and several linear regression models using local solvent exposures as inputs, based on a dataset of 1609 protein chains whose structures were resolved by NMR. The result again confirmed that the correlation of GNM outputs with raw RMSD values was better than that using B-factors of X-ray data. Nevertheless, it was also concluded that the parameter-free GNM and the solvent exposure based linear regression models performed worse than GNM when predicting RMSD, contrary to results using X-ray data. The discrepancy of residue flexibility prediction between NMR and X-ray data is likely attributable to a combination of their physical and methodological differences. 相似文献
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Caroline B. Albertin Laure Bonnaud C. Titus Brown Wendy J. Crookes-Goodson Rute R. da Fonseca Carlo Di Cristo Brian P. Dilkes Eric Edsinger-Gonzales Robert M. Freeman Jr. Roger T. Hanlon Kristen M. Koenig Annie R. Lindgren Mark Q. Martindale Patrick Minx Leonid L. Moroz Marie-Therese N?dl Spencer V. Nyholm Atsushi Ogura Judit R. Pungor Joshua J. C. Rosenthal Erich M. Schwarz Shuichi Shigeno Jan M. Strugnell Tim Wollesen Guojie Zhang Clifton W. Ragsdale 《Standards in genomic sciences》2012,7(1):175-188
The Cephalopod Sequencing Consortium (CephSeq Consortium) was established at a NESCent Catalysis Group Meeting, “Paths to Cephalopod Genomics- Strategies, Choices, Organization,” held in Durham, North Carolina, USA on May 24-27, 2012. Twenty-eight participants representing nine countries (Austria, Australia, China, Denmark, France, Italy, Japan, Spain and the USA) met to address the pressing need for genome sequencing of cephalopod mollusks. This group, drawn from cephalopod biologists, neuroscientists, developmental and evolutionary biologists, materials scientists, bioinformaticians and researchers active in sequencing, assembling and annotating genomes, agreed on a set of cephalopod species of particular importance for initial sequencing and developed strategies and an organization (CephSeq Consortium) to promote this sequencing. The conclusions and recommendations of this meeting are described in this white paper. 相似文献
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The influence of glucose, ammonium and magnesium availability on the production of protease and bacitracin by Bacillus licheniformis 总被引:1,自引:0,他引:1
Bacillus licheniformis was cultivated in a range of defined media varying in both the nature of the growth-limiting component and the concentration of excess nutrients. The compositions of the media were such as to ensure that the final absorbance (A430) of the culture was the same in each case. Samples taken during the stationary phase were assayed for their content of extracellular serine protease and bacitracin. The nature of the growth-limiting nutrient had a profound effect on the amounts of these products formed while those components which were present in excess also exerted an influence in proportion to their concentration. Thus, for example, a four-fold increase in serine protease production occurred when ammonium replaced glucose as the growth-limiting nutrient. Serine protease and bacitracin production responded differently to these varying cultural conditions suggesting they are subject to separate control mechanisms. The results are discussed in relation to the need for rigorously controlled cultural conditions during physiological studies of this nature. 相似文献
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