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991.
The building up of the two types of reaction centers, PS II and PS II, was investigated during the greening of Euglena gracilis Z cells in resting medium. The maximal values in the proportion of PS II centers (55%) and in the oxygen evolved per chlorophyll were reached at the outbreak of greening, when accumulation of galactolipids (MGDG and DGDG) rich in unsaturated fatty acids occurred, and when anionic lipids (SQDG and PG) emerged. As the greening progressed, the chlorophyll accumulation corresponded to a secondary enrichment in PS II centers, which built up more rapidly than PS II centers; correlatively, a general saturation of the fatty acids constitutive of all lipid classes took place.Abbreviations DCMU
3-(3,4-dichlorophenyl)-1,1-dimethylurea
- DGDG
digalactosyldiacylglycerol
- FAME
Tatty acid methyl esters
- HEPES
acide (N-[2-hydroxyethyl]piperazine-N-[2-ethane sulfonic]
- MGDG
monogalactosyldiacylglycerol
- PC
phosphatidylcholine
- PE
phosphatidylethanolamine
- PG
phosphatidylglycerol
- PQ
plastoquinone
- PS I
Photosystem I
- PS II
Photosystem II
- QA
primary quinone electron acceptor of PS II
- QB
secondary quinone electron acceptor of PS II
- SQDG
sulfoquinovosyldiacylglycerol 相似文献
992.
Accumulation of cadmium, lead and strontium, and a role of calcium oxalate in water hyacinth tolerance 总被引:2,自引:0,他引:2
Eichhornia crassipes plants brought from the River Nile were cultured in jars containing river water supplemented with various
concentrations of Cd, Pb, and Sr (0 to 100 μg cm-3), added simultaneously. Treatment continued for 20 d during which each
cultivation solution was being replaced with fresh one every 3 d. The growth of Eichhornia was drastically retarded at heavy
metal concentrations higher than 15 μg cm-3. At concentrations 15 or 25 μg cm-3, the accumulation of Cd and Pb to levels several
times higher than those in control plants was found. More than 50 % of the uptaken metals were retained by roots alone. Leaves
and leaf petiols received around 30 and 20 % of the accumulated metals, respectively. X-ray microanalysis indicated the presence
of the three heavy metals in Ca oxalate crystals. Content of metals in the crystals increased progressively over time of exposure
in a way similar to those in whole plant tissues. These results suggest a possible role for Ca oxalate crystalization in toxic
heavy metal deposition and thus tolerance by Eichhornia.
This revised version was published online in August 2006 with corrections to the Cover Date. 相似文献
993.
N Srairi-Abid P Mansuelle T Mejri H Karoui H Rochat F Sampieri M El Ayeb 《European journal of biochemistry》2000,267(17):5614-5620
Two toxin-like proteins (AahTL1 and AahTL3) were purified from the venom of the scorpion Androctonus australis Hector (Aah). AahTL1 and AahTL3 are the first non toxic proteins cross-reacting with AahI toxins group which indicates that these proteins can be used as a model of vaccins. In order to study structure-function relationships, their complete amino-acid sequences (66 residues) were determined, by automated Edman degradation. They show more than 50% of similarity with both AahI and AahIII antimammal toxins. Three-dimensional structural models of AahTL1 and AahTL3 constructed by homology suggest that the two proteins are structurally similar to antimammal scorpion alpha-toxins specific to voltage dependent Na+ channels. The models showed also that amino-acid changes between potent Aah toxins and both AahTL1 and AahTL3 disrupt the electrostatic potential gradient at their surface preventing their interaction with the receptor, which may explain their non toxicity. 相似文献
994.
The invasive brown seaweed Sargassum muticum as new resource for alginate in Morocco: Spectroscopic and rheological characterization 下载免费PDF全文
Samir El Atouani Fouad Bentiss Abdeltif Reani Rachid Zrid Zahira Belattmania Leonel Pereira Abdelhadi Mortadi Omar Cherkaoui Brahim Sabour 《Phycological Research》2016,64(3):185-193
The Japanese brown seaweed Sargassum muticum, recently invaded several shorelines worldwide including the Atlantic coast of Morocco with large well‐established populations. Within the framework of a sustainable strategy to control this invasive seaweed, we report on extraction yield, spectroscopic characterization and rheological properties of alginate, a commercially valuable colloid, from harvested biomass of S. muticum. Extraction yield was about 25.6% on dry weight basis. Infrared spectroscopy analysis shows that the obtained Fourier transform infrared spectra of the extracted biopolymer exhibit strong similarities with that of the commercial alginate. Furthermore, Proton nuclear magnetic resonance spectroscopy revealed that S. muticum alginate has almost equal amounts of β‐D‐mannuronic acid (M; 49%) and α‐L‐guluronic acid (G; 51%) with an M/G ratio of 1.04 and a high content of heteropolymeric MG GM diads suggesting a sequence distribution of an alternated polymer type. Rheological measurements were performed at different sodium alginate concentrations, temperatures and shear rates. The hydrocolloid exhibited pseudoplastic behavior and showed shear thinning, particularly at high solution concentration and low temperature which is consistent with the rheological behavior reported for commercial alginates. Considering the abundance of S. muticum in the Northwestern Atlantic coast of Morocco and the quality of the extracted hydrogel, this invasive species could be considered as a potential source of alginates. 相似文献
995.
996.
Current concepts concerning the social behavior of microorganisms inhabiting the human gastrointestinal (GI) tract and their role in the formation of integrated supracellular structures and in intercellular communication in the host–microbiota system are reviewed. The analysis of the literature data and of the results obtained by the authors indicates an important role of neuromediators (biogenic amines, amino acids, peptides, and nitric oxide) in intra- and interspecies microbial communication, as well as in the microbiota–host dialogue. The role of this dialogue for human health, its effect on the human psyche and social behavior, and the possibility of construction of probiotic preparations with a target-oriented neurochemical effect are discussed. 相似文献
997.
Andrzej Małobęcki Thomas Marcussen Jerzy Bohdanowicz Grzegorz Migdałek Aneta Słomka Elżbieta Kuta 《Botanical journal of the Linnean Society. Linnean Society of London》2016,182(1):180-194
Viola uliginosa is an endangered species throughout its range in Central Europe. Based on an unusual combination of morphological characters, including a reported lack of cleistogamous (CL) flowers, it was previously placed in the monotypic subsection Repentes, section Viola. Available individual phylogenetic analyses identify V. uliginosa as a lineage deeply embedded in section Viola, but hitherto its exact position was unknown. Here we confirm the occurrence of cleistogamy in V. uliginosa. Unlike other species of section Viola, V. uliginosa produced both chasmogamous and CL flowers at the same time, not at different times of the year (seasonal cleistogamy). The two flower types differed conspicuously in morphology and microstructure, especially in size and shape of the pistil and stamens as confirmed by scanning electron microscopy. Secondly, we resolved the phylogenetic position of V. uliginosa as a lineage deeply embedded in subsection Rostratae by means of a multigene multispecies coalescent analysis using *BEAST. In this analysis, V. mirabilis and V. uliginosa were successive sisters to the rest of subsection Rostratae and, because allopolyploids exist between V. mirabilis and the last one, subsection Rostratae needs to comprise all these taxa to be monophyletic. The evolution of key morphological traits is briefly discussed. 相似文献
998.
Laurence Mathieu Grégory Francius Racha El Zein Edith Angel Jean-Claude Block 《Biofouling》2016,32(8):925-934
The short-term kinetics of bacterial repopulation were evaluated after chlorination of high-density polyethylene (HDPE) colonized with drinking water biofilms and compared with bare HDPE surfaces. The effect of chlorination was partial as a residual biofilm persisted and was time-limited as repopulation occurred immediately after water resupply. The total number of bacteria reached the same levels on both the bare and chlorinated biofilm-fouled HDPE after a seven-day exposure to drinking water. Due to the presence of a residual biofilm, the hydrophobicity of chlorinated biofilm-fouled surface exhibited much lower adhesion forces (2.1 nN) compared to bare surfaces (8.9 nN). This could explain the rapid repopulation after chlorination, with a twofold faster bacterial accumulation rate on the bare HDPE surface. γ-Proteobacteria dominated the early stages of repopulation of both surfaces and a shift in the dominance occurred over the colonization time. Such observations define a timescale for cleaning frequency in industrial environments and guidelines for a rinsing procedure using drinking water. 相似文献
999.
Toxic gain of function from mutant FUS protein is crucial to trigger cell autonomous motor neuron loss 下载免费PDF全文
Jelena Scekic‐Zahirovic Oliver Sendscheid Hajer El Oussini Mélanie Jambeau Ying Sun Sina Mersmann Marina Wagner Stéphane Dieterlé Jérome Sinniger Sylvie Dirrig‐Grosch Kevin Drenner Marie‐Christine Birling Jinsong Qiu Yu Zhou Hairi Li Xiang‐Dong Fu Caroline Rouaux Tatyana Shelkovnikova Anke Witting Albert C Ludolph Friedemann Kiefer Erik Storkebaum Clotilde Lagier‐Tourenne Luc Dupuis 《The EMBO journal》2016,35(10):1077-1097
FUS is an RNA‐binding protein involved in amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD). Cytoplasmic FUS‐containing aggregates are often associated with concomitant loss of nuclear FUS. Whether loss of nuclear FUS function, gain of a cytoplasmic function, or a combination of both lead to neurodegeneration remains elusive. To address this question, we generated knockin mice expressing mislocalized cytoplasmic FUS and complete FUS knockout mice. Both mouse models display similar perinatal lethality with respiratory insufficiency, reduced body weight and length, and largely similar alterations in gene expression and mRNA splicing patterns, indicating that mislocalized FUS results in loss of its normal function. However, FUS knockin mice, but not FUS knockout mice, display reduced motor neuron numbers at birth, associated with enhanced motor neuron apoptosis, which can be rescued by cell‐specific CRE‐mediated expression of wild‐type FUS within motor neurons. Together, our findings indicate that cytoplasmic FUS mislocalization not only leads to nuclear loss of function, but also triggers motor neuron death through a toxic gain of function within motor neurons. 相似文献
1000.
Rasha Y. Mahmoud Daniel Henry Stones Wenqin Li Mohamed Emara Ramadan A. El‐domany Depu Wang Yili Wang Anne Marie Krachler Jun Yu 《Molecular microbiology》2016,99(4):658-673
Shigella sonnei is a bacterial pathogen and causative agent of bacillary dysentery. It deploys a type III secretion system to inject effector proteins into host epithelial cells and macrophages, an essential step for tissue invasion and immune evasion. Although the arsenal of bacterial effectors and their cellular targets have been studied extensively, little is known about the prerequisites for deployment of type III secreted proteins during infection. Here, we describe a novel S. sonnei adhesin, SSO1327 which is a multivalent adhesion molecule (MAM) required for invasion of epithelial cells and macrophages and for infection in vivo. The S. sonnei MAM mediates intimate attachment to host cells, which is required for efficient translocation of type III effectors into host cells. SSO1327 is non‐redundant to IcsA; its activity is independent of type III secretion. In contrast to the up‐regulation of IcsA‐dependent and independent attachment and invasion by deoxycholate in Shigella flexneri, deoxycholate negatively regulates IcsA and MAM in S. sonnei resulting in reduction in attachment and invasion and virulence attenuation in vivo. A strain deficient for SSO1327 is avirulent in vivo, but still elicits a host immune response. 相似文献