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71.
Lavandula genus is an important member of Labiatae (Lamiaceae) family. People use commonly Lavandula stoechas as a medicinal plant for various diseases around the world and also in Turkey. The aim of this study was to investigate cytotoxic
and genotoxic effects of aqueous extracts (40, 80 and 120 g/L) from L. stoechas flowers on Allium cepa root tip meristem cells. For this purpose, A. cepa onion bulbs were treated with the above-mentioned L. stoechas flower extracts for 72 h. Spring water (pH 7.3) was used as a control. The result of this study sowed that aqueous extracts
reduced mitotic index, but induced chromosome aberrations and mitotic aberrations in comparison with control, significantly
(p < 0.05). Aqueous extracts induced breaks, stickiness, pole deviations and micronuclei. Furthermore, these effects were related
to extract concentrations. These results showed that L. stoechas aqueous extracts have cytotoxic and genotoxic effects. 相似文献
72.
73.
Prucalopride (PCD), is a modern medication approved by the United States in 2018 to alleviate constipation caused by motility issues. PCD demonstrates a strong affinity and selectivity toward the 5-HT4 receptor. The study here introduces a feasible, direct, non-extractive, and affordable pathway for PCD analytical tracking. The fluorimetric study is based on the on–off effect on the emission amplitude of fluorone-based dye (pyrosin B). In a one-pot experiment, the complex between PCD and pyrosin B is formed instantly in an acidic medium. Correlation between decreased pyrosin B emission and PCD concentrations provides a linear calibration plot from 50 to 900 ng/mL. PCD–dye complex system affecting variables were meticulously tuned. The values of the estimated limit of quantitation and limit of detection for the current methodology were 47.5 and 15.7 ng/mL, respectively. Conformity of the strategy validity was achieved by a comprehensive study of the International Council for Harmonization of Technical Requirements for Pharmaceuticals for Human Use criteria. The method was convincingly applied for PCD assay in tablets and content uniformity investigation. Furthermore, PCD tracking in the spiked biological fluid was applied. Finally, the method uses distilled water as dispersing medium which rise accommodation with the green chemistry principle. 相似文献
74.
Habibul Bari Shozib Md. Mariful Islam Sultan Abu Saleh Mahmud Md. Nazmul Bari Nasima Akter Saima Jahan Shakir Hosen Mohammad Nazir Hossain A.H.M. Nurun Nabi Muhammad Ali Siddiquee Md. Manjurul Haque 《Saudi Journal of Biological Sciences》2021,28(12):7472-7480
BackgroundBlack pericarp rice has recently become popular among rice consumers for its diverse health benefits specially anti-cancer effect. Cyanidin-3-Glucosides (C3G), an prominant bioactive component of anthocyanins which is abundantly present in black pericarp rice.ObjectivesWe investigated, how effectively it can be used to fortify Cyanidin-3-Glucosides (C3G) content in red and white pericarp polished rice or rice based bakery products for more nutritional value.MethodIn the present study, we have characterized several black pericarp rice cultivars along with some red pericarp and white pericarp rice cultivars by physicochemical including mineral profiling, and quantified the C3G by UFLC and LCMS.ResultsC3G content was significantly reduced from raw rice to cooked rice condition. All the black pericarp rice cultivars synthesized C3G, while this content was not detected in red and white pericarp rice cultivars. However, when 25% of black pericarp rice were mixed with 75% red or white pericarp polished rice, C3G content was significantly retained in cooked rice conditions. Formulation of rice-based bakery food product using black pericarp rice powder was also remarkably retained the C3G content as compared to that of cooking. Black rice is harder in texture, difficult to digest and needs higher energy for cooking. Therefore, we tried to circumvent these challenges by fortifying 25% of black pericarp rice with white or red pericarp rice.ConclusionFortification of C3G enriched black rice (25%) with red or white pericarp rice (75%) might bring a better nutritional quality in both cooking and baking condition. This may lead a way to the effective management of the non-communicable disease such as cancer for common rice consuming population. 相似文献
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77.
Rehman S Shawl AS Verma V Kour A Athar M Andrabi R Sultan P Qazi GN 《Prikladnaia biokhimiia i mikrobiologiia》2008,44(2):225-231
The medicinal plant, Nothapodytes foetida contains a number of important alkaloids like camptothecin (an anticancer drug molecule) but its concentration is less to meet the existing demand of this important molecule, so in an effort for accessible availability of camptothecin. An endophyte (designated ZP5SE) was isolated from the seed of Nothapodytes foetida and was examined as potential source of anticancer drug lead compound i.e. camptothecin, when grown in Sabouraud liquid culture media under shake flask conditions. The presence of anticancer compound (camptothecin) in this fungus was confirmed by chromatographic and spectroscopic methods in comparison with authentic camptothecin. Isolated endophyte (Neurospora crassa) producing camptothecin may become an easily accessible source for the production of precursor anticancer drug molecule in future at large scale. 相似文献
78.
Syed Sultan Beevi Lakshmi Narasu Mangamoori Naveen Anabrolu 《World journal of microbiology & biotechnology》2009,25(3):465-473
Aqueous, methanol, ethyl acetate, and chloroform extracts of the root, stem, and leaf of Raphanus sativus were studied for antibacterial activity against food-borne and resistant pathogens. All extracts except the aqueous extracts
had significant broad-spectrum inhibitory activity. The ethyl acetate extract of the root had the potent antibacterial activity,
with a minimum inhibitory concentration (MIC) of 0.016–0.064 mg/ml and a minimum bactericidal concentration (MBC) of 0.016–0.512 mg/ml
against health-damaging bacteria. This was followed by the ethyl acetate extracts of the leaf and stem with MICs of 0.064–0.256
and 0.128–0.256 mg/ml, respectively and MBCs of 0.128–2.05 and 0.256–2.05 mg/ml, respectively. The ethyl acetate extracts
of the different parts of R. sativus retained their antibacterial activity after heat treatment at 100°C for 30 min, and their antibacterial activity was enhanced
when pH was maintained in the acidic range. Hence this study, for the first time, demonstrated that the root, stem, and leaf
of R. sativus had significant bactericidal effects against human pathogenic bacteria, justifying their traditional use as anti-infective
agents in herbal medicines. 相似文献
79.
短毛柽柳不同部位中总黄酮含量及稳定性研究 总被引:1,自引:0,他引:1
目的:比较短毛柽柳中不同部位总黄酮的含量差异,研究黄酮化合物保存的条件,方法:采用超声波乙醇浸提法,在正交优先的最佳条件(料液比为1∶15,超声处理时间为40min,乙醇浓度为45%,超声温度50℃,提取3次)下提取不同部位总黄酮,并测定不同条件下其吸光度的变化。结果:柽柳花、叶、茎中总黄酮得率分别为24.585mg/g、25.623mg/g、41.726mg/g,柽柳中提取的黄酮溶液在光照、高温、高浓度蔗糖溶液和Na+存在条件下不稳定;pH 4~7条件下较稳定;氧化剂对其稳定性的影响不大。结论:柽柳茎中总黄酮含量明显高于其他两个部位。柽柳黄酮保存时应避免接触碳水化合物和盐离子,在避光、常温、中性条件下保存。 相似文献
80.
Serageldeen Sultan Nguyen Thi Lan Toshiki Ueda Ryoji Yamaguchi Ken Maeda Kazushige Kai 《Acta veterinaria Scandinavica》2009,51(1):38