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排序方式: 共有83条查询结果,搜索用时 15 毫秒
31.
Nahid Shahabadi Mohammad Hakimi Teimoor Morovati Navid Fatahi 《Nucleosides, nucleotides & nucleic acids》2017,36(8):497-510
The interaction of a novel macrocyclic copper(II) complex, ([CuL(ClO4)2] that L is 1,3,6,10,12,15-hexaazatricyclo[13.3.1.16,10]eicosane) with calf thymus DNA (ct-DNA) was investigated by various physicochemical techniques and molecular docking at simulated physiological conditions (pH = 7.4). The absorption spectra of the Cu(II) complex with ct-DNA showed a marked hyperchroism with 10 nm blue shift. The intrinsic binding constant (Kb) was determined as 1.25 × 104 M?1, which is more in keeping with the groove binding with DNA. Furthermore, competitive fluorimetric studies with Hoechst33258 have shown that Cu(II) complex exhibits the ability to displace the ct-DNA-bound Hoechst33258 indicating that it binds to ct-DNA in strong competition with Hoechst33258 for the groove binding. Also, no change in the relative viscosity of ct-DNA and fluorescence intensity of ct-DNA-MB complex in the present of Cu(II) complex is another evidence to groove binding. The thermodynamic parameters are calculated by van't Hoff equation, which demonstrated that hydrogen bonds and van der Waals interactions played major roles in the binding reaction. The experimental results were in agreement with the results obtained via molecular docking study. 相似文献
32.
[3H]ouabain binding and 86rubidium uptake during the cell growth cycle in L5178Y murine lymphoblasts
There was a parallel alteration in [3H]ouabain binding and 86Rb+ uptake during the cell growth cycle in L5178Y murine lymphoblasts. The initial rate of Rb+ uptake and [3H]ouabain binding was highest at the stationary phase of the cell growth cycle. The possible relationship between changes in cation transport and membrane properties to the cell growth cycle is discussed. 相似文献
33.
F Riske J Hakimi M Mallamaci M Griffin B Pilson N Tobkes P Lin W Danho J Kochan R Chizzonite 《The Journal of biological chemistry》1991,266(17):11245-11251
The binding of IgE to the high affinity Fc epsilon receptor (Fc epsilon RI) on mast cells and basophils is mediated by the alpha-subunit of the tetrameric receptor complex. Based on sequence homologies, the 50-kDa alpha-subunit is a member of the immunoglobulin superfamily of proteins and has two predicted disulfide-bonded loops. Monoclonal antibodies specific for the human alpha-subunit have been identified and separated into two major classes: inhibitory and noninhibitory antibodies. Inhibitory antibodies (i.e. 15A5) block 125I-IgE binding to a recombinant chimeric alpha-subunit (ch-alpha-protein) expressed on Chinese hamster ovary cells and immunoprecipitate 125I-labeled purified ch-alpha-protein. Noninhibitory antibodies (i.e. 22E7) immunoprecipitate both 125I-labeled ch-alpha-protein and the soluble complex of 125I-IgE cross-linked to ch-alpha-protein but do not block 125I-IgE binding to the ch-alpha-protein expressed on Chinese hamster ovary cells. Both classes of antibodies bind to natural Fc epsilon RI present on human basophils and induce histamine release from these cells. Inhibitory antibody 15A5 specifically binds to a peptide corresponding to amino acids 125-140 of the putative second domain of the alpha-subunit sequence. All the inhibitory antibodies compete with 125I-15A5 for binding to the ch-alpha-protein, indicating that these antibodies recognize inhibitory epitopes that are either identical or sterically overlapping. Noninhibitory antibodies (i.e. 22E7) do not block 125I-15A5 binding to the ch-alpha-protein. These data suggest that antibodies binding to the predicted second domain of the alpha-subunit can inhibit IgE binding to the alpha-subunit, while antibodies binding at a distance from this site do not inhibit IgE binding. These inhibitory antibodies may block IgE binding to the ch-alpha-protein by direct overlap, steric inhibition, or induced conformational changes of the receptor contact points for IgE. 相似文献
34.
M Giralt E A Park A L Gurney J S Liu P Hakimi R W Hanson 《The Journal of biological chemistry》1991,266(32):21991-21996
35.
The alpha subunit of the human IgE receptor (FcERI) is sufficient for high affinity IgE binding 总被引:7,自引:0,他引:7
J Hakimi C Seals J A Kondas L Pettine W Danho J Kochan 《The Journal of biological chemistry》1990,265(36):22079-22081
The alpha subunit of the FcERI binds IgE with high affinity. Previous studies have demonstrated that alpha subunit expression requires the presence of beta and/or gamma subunits, and it is not known how these two subunits contribute to the ability of the alpha subunit to bind IgE. In this report, we describe the expression and characterization of a human chimeric alpha subunit. The data demonstrate that high affinity IgE binding does not require the presence of the beta and/or gamma subunits and that this activity is localized to the extracellular domain (residues 26-201) of the human alpha subunit. Permanent cell lines expressing the chimeric receptor were used to characterize the binding parameters of the alpha subunit. These cell lines provide a means of identifying therapeutic agents which may be effective in the treatment/management of allergic diseases. 相似文献
36.
37.
Marcel Betsch Michael Wild Brian Johnstone Pascal Jungbluth Mohssen Hakimi Britta Kühlmann Walter Rapp 《PloS one》2013,8(7)
Introduction
The assessment of spinal deformities with rasterstereography can enhance the understanding, as well as can reduce the number of x-rays needed. However, to date this technique only allows measurements under static conditions. Since it would be of great value to be able to also analyze the spine in dynamic conditions, the present study evaluated a novel rasterstereographic system.Materials and Methods
A new rasterstereographic device was evaluated in a comparison with the gold standard in motion analysis, the VICON system. After initial testing using 12 flat infrared markers adhered to a solid plate, the two systems were evaluated with the markers adhered onto the backs of 8 test subjects. Four triangles were defined using the markers, and the sides of each triangle were measured under static and dynamic conditions.Results
On the solid plate, the sides of the 4 triangles were measured with a measuring tape and then by the two optical systems. Rasterstereography showed a high accuracy in marker detection on the solid plate. Under dynamic conditions, with the subjects walking on a treadmill, the rasterstereographically-measured side lengths were compared with the lengths measured by the VICON system as an assessment of marker detection. No significant differences (p>0.05) were found between the systems, differing only 0.07–1.1% for all sides of the four triangles with both systems.Discussion
A novel rasterstereographic measurement device that allows surface and spine topography under dynamic conditions was assessed. The accuracy of this system was with one millimeter on a solid plate and during dynamic measurements, to the gold standard for motion detection. The advantage of rasterstereography is that it can be used to determine a three-dimensional surface map and also allows the analysis of the underlying spine. 相似文献38.
Yimin Qian Karin Conde-Knape Shawn D. Erickson Fiorenza Falcioni Paul Gillespie Irina Hakimi Francis Mennona Yonglin Ren Hamid Salari Sung-Sau So Jefferson W. Tilley 《Bioorganic & medicinal chemistry letters》2013,23(14):4216-4220
Benzimidazole and indane are the two key fragments in our potent and selective MCH-1 receptor (MCHR1) antagonists. To identify novel linkers connecting the two fragments, we investigated diamino-cycloalkane-derived analogs and discovered highly potent antagonists with cis-1,4-diaminocyclohexane as a unique spacer in this chemical class. Structural overlay suggested that cis-1-substituted-4-aminocyclohexane functions as a bioisostere of 4-substituted-piperidine and that the active conformation adopts a U-shaped orientation. 相似文献
39.
Ye F Lemieux H Hoppel CL Hanson RW Hakimi P Croniger CM Puchowicz M Anderson VE Fujioka H Stavnezer E 《The Journal of biological chemistry》2011,286(46):40013-40024
40.
Erickson SD Banner B Berthel S Conde-Knape K Falcioni F Hakimi I Hennessy B Kester RF Kim K Ma C McComas W Mennona F Mischke S Orzechowski L Qian Y Salari H Tengi J Thakkar K Taub R Tilley JW Wang H 《Bioorganic & medicinal chemistry letters》2008,18(4):1402-1406
This paper describes the lead optimization of a new series of potent, selective, orally bioavailable, brain-penetrant MCH-1 receptor antagonists. A major focus of the work was to achieve a selectivity profile appropriate for in vivo efficacy studies and safety. 相似文献