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HARUKAZU NAKAMURA HAJIME FUJISAWA KENJI WATANABE SEIICHI KUBO YASUHIKO IBATA 《Development, growth & differentiation》1978,20(1):49-54
Clonal culture of muscle cells from thigh muscles of the term fetus of the mouse was undertaken. Myoblasts were spherical or spindle-shaped, and proliferated exponentially until day 4 in culture. The generation time of the muscle cells from 2 to 4 days' culture was 9.1 to 13.4 hr. The fusion of myoblasts began on day 4 in culture; many myotubes had been formed by day 6 and spontaneous contraction was observed on day 7. Clonal efficiency was 30%, and the proportion of muscle colonies in all the colonies was 72%. 相似文献
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TOSHIHIRO WATANABE RAN KOUHO TOMO KATAYOSE NOBUYUKI KITAJIMA NAOYA SAKAMOTO NORIKO YAMAGUCHI TAKURO SHINANO HISAYOSHI YURIMOTO MITSURU OSAKI 《Plant, cell & environment》2014,37(1):45-53
Low‐molecular‐weight thiol (LMWT) synthesis has been reported to be directly induced by arsenic (As) in Pteris vittata, an As hyperaccumulator. Sulphur (S) is a critical component of LMWTs. Here, the effect of As treatment on the uptake and distribution of S in P. vittata was investigated. In P. vittata grown under low S conditions, the presence of As in the growth medium enhanced the uptake of SO42?, which was used for LMWT synthesis in fronds. In contrast, As application did not affect SO42? uptake in Nephrolepis exaltata, an As non‐hyperaccumulator. Moreover, the isotope microscope system revealed that S absorbed with As accumulated locally in a vacuole‐like organelle in epidermal cells, whereas S absorbed alone was distributed uniformly. These results suggest that S is involved in As transport and/or accumulation in P. vittata. X‐ray absorption near‐edge structure analysis revealed that the major As species in the fronds and roots of P. vittata were inorganic As(III) and As(V), respectively, and that As–LMWT complexes occurred as a minor species. Consequently, in case of As accumulation in P. vittata, S possibly acts as a temporary ligand for As in the form of LMWTs in intercellular and/or intracellular transport (e.g. vacuolar sequestration). 相似文献
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M. DEMURA M. KAWACHI M. KUNUGI T. NISHIZAWA F. KASAI M. M. WATANABE 《Molecular ecology resources》2007,7(2):315-317
We have developed 11 microsatellite markers that are specific to Chattonella antiqua, C. marina, and C. ovata, the red tide‐forming harmful phytoplanktons. The 11 loci were amplified in the three species. The number of alleles per locus ranged from 5 to 16. The three species shared most microsatellite regions, although the genetic differences in specific loci were detected among them. These markers of the Chattonella species will be beneficial for biogeographical, detailed taxonomic, studies. 相似文献
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Females of the swallowtail butterfly Papilio xuthus L. (Lepidoptera: Papilionidae) mate multiply during their life span and use the spermatophores transferred to increase their longevity as well as fecundity. Sperm from different males may be stored in the sperm storage organs (bursa copulatrix and spermatheca). To clarify the pattern of sperm storage and migration in the reproductive tract, mated females are dissected after various intervals subsequent to the first mating, and the type and activity of sperm in the spermatheca are observed. When virgin females are mated with virgin males, the females store sperm in the spermatheca for more than 10 days. Sperm displacement is found in females that are remated 7 days after the first mating. Immediately after remating, these females flush out the sperm of the first male from the spermatheca before sperm migration of the second male has started. However, females receiving a small spermatophore at the second mating show little sperm displacement, and the sperm derived from the small spermatophore might not be able to enter the spermatheca. Females appear to use spermatophore size to monitor male quality. 相似文献
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TAKAYUKI KARAKI YOKO WATANABE TETSUYA KONDO TAKAYOSHI KOIKE 《Plant Species Biology》2012,27(3):226-232
In Japan the black locust (Robinia pseudoacacia L.) is undergoing rapid habitat expansion, which has an adverse effect on native vegetation. It is therefore a priority to clarify the regeneration characteristics of the black locust and establish adequate management of this invasive species in Japan. To determine the germination characteristics of physically dormant black locust seeds, we observed anatomical features of the seed coat and identified the water gap that acts as a signal detector. Our microscopic observations showed that seed coats of this species had hilum, micropyle and strophiole. The anatomical features of these regions correspond to the general characteristics of papilionoid legume seeds. Based on our microscopic observations, water absorption blocking experiments and a dye tracking experiment, we identified the strophiole as a water gap in black locust seeds. Our results suggest that the opening of the strophiole is important for water uptake to the embryo and subsequent germination of black locust seeds under natural conditions. 相似文献