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DNA extracted from macronuclei of axenically cultured Paramecium aurelia has been characterized with regard to its kinetic complexity. Renaturation of macronuclear DNA from this protozoon appeared to follow 2nd order kinetics and revealed the presence of 2 components: a main component comprising ~96% of the genome which renatured slowly and a minor component comprising ~4% of the genome which renatured at a rate ~3000 faster than the main component. The value of the kinetic complexity of the main component has been estimated at 3.8 × 1010 daltons and that of the minor component at 1.45 × 107 daltons. It is suggested that the macronucleus contains ~840 diploid copies of the slowly renaturing component; for each copy of the latter there are ~100 copies of the fast renaturing component. 相似文献
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CATALFAMO JAMES L.; FEINBERG JOSEPH H.; SMITH GEORGE W.; BIRECKA HELENA 《Journal of experimental botany》1978,29(2):347-357
Ethylene at 580 µl l1 inhibited elongationand induced swelling in internodes of light-grown normal anddwarf pea plants; GA3 did not prevent swelling in response toethylene. GA3 neither inhibited nor enhanced the activity of isoperoxidasesin the internodes, regardless of its effect on their elongation.Ethylene at 80 µl l1 enhanced peroxidase in GA3-untreatedand treated normal and dwarf plants. At 5 µl l1,ethylene had only a weak effect on peroxidase activity or none.The enzyme enhancement by ethylene was not related to its effecton cell expansion and seems do be due, at least in part, tochemical injury. Electron microscopy revealed peroxidase activity in the roughER and cell walls, including intercellular spaces. Stainingof walls in ethylene-treated tissues was more pronounced thanin untreated ones. Golgi vesicles did not seem to be involvedin the assembly of the enzyme carbohydrate moiety in ethylene-treatedcells. The peroxidase fraction extracted with 20 mM phosphate buffer,pH 6, and that extracted from wall debris with 1 M NaCl accountedfor 98% of total enzyme activity. Both fractions contained thesame six cathodic isoforms which comprised 8590% of theiractivity. Electrophoresis did not reveal differences in thequalitative isoenzyme patterns in relation to variety, age,GA3, or ethylene. The only observed quantitative differenceswere age-dependent. Procedural artefacts during separation of protoplast and wallionically bound peroxidase fractions are discussed. 相似文献
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SÓNIA VIEIRA HELENA COELHO RITA NOLASCO JOÃO SERÔDIO RICHARD BARNES HENRIQUE QUEIROGA 《Biological journal of the Linnean Society. Linnean Society of London》2010,100(2):439-450
Intertidal animals display a suite of cyclic behaviours that evolved as adaptations to the predictable cycle of inundation and exposure. In estuarine habitats, mud snails from the genus Hydrobia are among the most abundant grazers, and have received considerable attention with respect to the behavioural mechanisms mediating locomotion, dispersal, and feeding, although the nature of the control of these processes has remained elusive. In particular, it is not clear whether endogenous activity patterns are related to periodic changes of microphytobenthos biomass at the sediment surface, or whether they are timed to the tidal cycle at all. In the present study, we address the crawling activity of Hydrobia ulvae under constant conditions, as well as the effects of individual size and previous short‐term exposure to tides of different range, by recording immersed individual snails under constant dark conditions. We show that the species displays an overt circatidal pattern of crawling, with activity peaks around high water, and that the start of inundation may act as an entrainment agent of the rhythm. Moreover, the results obtained indicate that smaller snails display higher levels of activity, although neither the size nor previous in situ influence of tidal range has an effect on the period and on the amplitude of the rhythm. These findings suggest that fluctuations of microphytobenthos biomass are not a sufficiently strong selective pressure to have shaped locomotor activity in H. ulvae. Moreover, feeding of H. ulvae should take place mostly during high water and be independent of periodic fluctuations of microphytobenthos biomass at the surface of the sediment. © 2010 The Linnean Society of London, Biological Journal of the Linnean Society, 2010, 100 , 439–450. 相似文献
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HELENA EKLUND 《Botanical journal of the Linnean Society. Linnean Society of London》2000,132(4):397-428
Three new taxa with clear affinity to extant Lauraceae are described from the Santonian/ Campanian ( c .83 My, Late Cretaceous) Neuse River locality in North Carolina, U.S.A. A new lauraceous genus, Neusenia , is established to accommodate an excellently preserved flower with tetrasporangiate anthers and psilate pollen grains. Two additional lauraceous taxa are described but not named due to incomplete preservation. The fossil taxa described in this paper represent a variety of evolutionary lineages within Lauraceae with respect to inflorescence structure and anther morphology, including both distinctly pedicellate flowers and sessile, closely crowded flowers, as well as tetrasporangiate and disporangiate anthers. In light of the co-occurrence of both tetrasporangiate and disporangiate anthers in the Neuse River flora, the plesiomorphic state of lauraceous anthers is discussed. Mapped on a recent cladogram of Laurales, tetrasporangiate anthers appear to be primitive within Lauraceae. Thus, disporangiate 2-valvate anthers must have evolved independently at least three times in Laurales (in Lauraceae, Hernandiaceae, and Atherosperrnataceae/Gomortegaceae). In Hernandiaceae and Atherosperrnataceae/Gomortegaceae such anthers are interpreted to have originated from tetrasporangiate 2-valvate anthers through reduction of the septum in each theca, while in Lauraceae they may have originated in the same way and/or from reduction of two pollen sacs in a tetrasporangiate 4-valvate anther. 相似文献
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HANNU HUUSKONEN HELENA HAAKANA JUKKA KEKÄLÄINEN 《Biological journal of the Linnean Society. Linnean Society of London》2009,98(3):532-539
The 'good genes' hypothesis predicts that males advertise their quality with different sexual ornaments and that females are able to recognize the genetic quality of males by evaluating these characteristics. In the present study, we investigated the parental effects on offspring performance (feeding and swimming ability of newly-hatched larvae) and examined whether male ornamentation indicates offspring success in performance trials of whitefish ( Coregonus lavaretus Linnaeus). Offspring first-feeding success had a strong paternal effect and it was also positively correlated with the size of male breeding tubercles, indicating that breeding ornamentation of males can function as an honest indicator of their genetic quality. In addition, the observed positive correlation between male tubercle size and condition factor suggests that highly ornamented males are efficient foragers and that this trait may have a heritable basis. By contrast to feeding success, only a maternal effect was found in the swimming ability of the larvae. Clear family-specific differences observed in both measures of performance strongly suggest that parental identity may have important effects on larval survival in the wild. © 2009 The Linnean Society of London, Biological Journal of the Linnean Society , 2009, 98 , 532–539. 相似文献
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FREDRIK PLEIJEL GREG W. ROUSE CHRISTINE RUTA HELENA WIKLUND ARNE NYGREN 《Zoological Journal of the Linnean Society》2008,152(4):625-634
Vrijenhoekia balaenophila gen. nov., sp. nov. (Polychaeta, Hesionidae) is described from a whale carcass at near 3000 m depth in Monterey Canyon off the coast of California. The phylogenetic relationships of V. balaenophila are assessed in a parsimony analysis of morphological data together with nucleotide data from 28S rDNA, 16S rDNA and cytochrome oxidase I genes. Within the hesionids V. balaenophila belongs to Psamathini, where it is the sister group to Sirsoe . Among psamathins it is morphologically distinguished by having six glandular lip pads around the mouth opening, papilla-shaped neuropodial lobes on segment 3, extreme length of the dorsal cirri, and by a characteristic growth pattern in which the maximum number of segments is already formed in subadults, and further growth takes place through size increase of the segments. © 2008 The Linnean Society of London, Zoological Journal of the Linnean Society , 2008, 152 , 625–634. 相似文献
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I. HELENA ANDERSSON 《Physiological Entomology》1992,17(3):203-207
Abstract. Starved females of Aedes communis (DeGeer) had significantly shorter mean lifespans than females with access to one blood meal and/or sugar meals. For the starving females, lifespan was significantly correlated with wing length. This correlation was not found in sugar- and/or blood-fed females. Fructose and sucrose had similar effects on mosquito lifespan. The 25% and 50% (w/w) solutions of each, increased mean lifespans to 37.8 ± 7.2 and 34.9 ± 9.1 days, respectively. A 10% solution, of either sugar, was significantly inferior, mean lifespan 23.2 ± 10.2 days. The initial sugar meal was very important for female longevity. Subsequent meals aided in ovarian development. One sugar meal, one blood meal, and several sugar meals during oogenesis was found to be the optimal diet for the female mosquitoes in this study. 相似文献