全文获取类型
收费全文 | 206篇 |
免费 | 25篇 |
国内免费 | 2篇 |
出版年
2021年 | 6篇 |
2019年 | 4篇 |
2018年 | 6篇 |
2017年 | 7篇 |
2016年 | 4篇 |
2015年 | 8篇 |
2014年 | 11篇 |
2013年 | 12篇 |
2012年 | 12篇 |
2011年 | 11篇 |
2010年 | 13篇 |
2009年 | 11篇 |
2008年 | 7篇 |
2007年 | 3篇 |
2006年 | 7篇 |
2005年 | 9篇 |
2004年 | 3篇 |
2003年 | 3篇 |
2002年 | 4篇 |
2001年 | 3篇 |
2000年 | 2篇 |
1999年 | 2篇 |
1998年 | 8篇 |
1997年 | 8篇 |
1996年 | 4篇 |
1994年 | 2篇 |
1993年 | 6篇 |
1992年 | 4篇 |
1990年 | 3篇 |
1988年 | 5篇 |
1985年 | 3篇 |
1984年 | 3篇 |
1982年 | 3篇 |
1975年 | 1篇 |
1974年 | 2篇 |
1973年 | 1篇 |
1971年 | 1篇 |
1970年 | 2篇 |
1969年 | 1篇 |
1968年 | 1篇 |
1882年 | 1篇 |
1881年 | 2篇 |
1878年 | 2篇 |
1875年 | 2篇 |
1874年 | 2篇 |
1872年 | 1篇 |
1871年 | 2篇 |
1862年 | 2篇 |
1861年 | 3篇 |
1859年 | 2篇 |
排序方式: 共有233条查询结果,搜索用时 46 毫秒
41.
Kazuhiro Sato Fumitaka Abe Martin Mascher Georg Haberer Heidrun Gundlach Manuel Spannagl Kenta Shirasawa Sachiko Isobe 《DNA research》2021,28(3)
We have established a high-quality, chromosome-level genome assembly for the hexaploid common wheat cultivar ‘Fielder’, an American, soft, white, pastry-type wheat released in 1974 and known for its amenability to Agrobacterium tumefaciens-mediated transformation and genome editing. Accurate, long-read sequences were obtained using PacBio circular consensus sequencing with the HiFi approach. Sequence reads from 16 SMRT cells assembled using the hifiasm assembler produced assemblies with N50 greater than 20 Mb. We used the Omni-C chromosome conformation capture technique to order contigs into chromosome-level assemblies, resulting in 21 pseudomolecules with a cumulative size of 14.7 and 0.3 Gb of unanchored contigs. Mapping of published short reads from a transgenic wheat plant with an edited seed-dormancy gene, TaQsd1, identified four positions of transgene insertion into wheat chromosomes. Detection of guide RNA sequences in pseudomolecules provided candidates for off-target mutation induction. These results demonstrate the efficiency of chromosome-scale assembly using PacBio HiFi reads and their application in wheat genome-editing studies. 相似文献
42.
Angela Karp Steve J.Hanley Sviatlana O.Trybush William Macalpine Ian Shield 《Acta Botanica Sinica》2011,(2)
Willows (Salix spp.) are a very diverse group of catkin-bearing trees and shrubs that are widely distributed across temperate regions of the globe.Some species respond well to being grown in short rotation coppice (SRC) cycles,which are much shorter than conventional forestry.Coppicing reinvigorates growth and the biomass rapidly accumulated can be used as a source of renewable carbon for bioenergy and biofuels.As SRC willows re-distribute nutrients during the perennial cycle they require only minimal nitro... 相似文献
43.
Ohne Zusammenfassung 相似文献
44.
45.
J. Gundlach 《Journal of Ornithology》1859,7(4):294-299
Ohne Zusammenfassung 相似文献
46.
Sarah C. R. Lummis rew L. Gundlach Graham A. R. Johnston Peter A. W. Harper Peter R. Dodd 《Journal of neurochemistry》1990,55(2):421-426
Inherited congenital myoclonus (ICM) of Poll Hereford cattle is a neurological disease in which there are severe alterations in spinal cord glycine-mediated neurotransmission. There is a specific and marked decrease, or defect, in glycine receptors and a significant increase in neuronal (synaptosomal) glycine uptake. Here we have examined the characteristics of the cerebral gamma-aminobutyric acid (GABA) receptor complex, and demonstrate that the malfunction of the spinal cord inhibitory system is accompanied by a change in the major inhibitory system in the cerebral cortex. In synaptic membrane preparations from ICM calves, both high-and low-affinity binding sites for the GABA agonist [3H]muscimol were found (KD = 9.3 +/- 1.5 and 227 +/- 41 nM, respectively), whereas only the high-affinity site was detectable in controls (KD = 14.0 +/- 3.1 nM). The density and affinity of benzodiazepine agonist binding sites labelled by [3H]diazepam were unchanged, but there was an increase in GABA-stimulated benzodiazepine binding. The affinity for t-[3H]butylbicyclo-o-benzoate, a ligand that binds to the GABA-activated chloride channel, was significantly increased in ICM brain membranes (KD = 148 +/- 14 nM) compared with controls (KD = 245 +/- 33 nM). Muscimol-stimulated 36Cl- uptake was 12% greater in microsacs prepared from ICM calf cerebral cortex, and the uptake was more sensitive to block by the GABA antagonist picrotoxin. The results show that the characteristics of the GABA receptor complex in ICM calf cortex differ from those in cortex from unaffected calves, a difference that is particularly apparent for the low-affinity, physiologically relevant GABA receptors.(ABSTRACT TRUNCATED AT 250 WORDS) 相似文献
47.
Mycotoxin Research - Six laboratories analyzed portions of the same aqueous acetonitrile extracts of three peanut butters for aflatoxin concentrations by an HPLC procedure (using immunoaffinity... 相似文献
48.
Oocysts of Eimeria saudiensis n. sp. (Apicomplexa: Eimeriidae) are described from the feces of the Arabian oryx, Oryx leucoryx , from the Riyadh Zoo, Saudi Arabia. The oocysts were ellipsoidal or slightly ovoid, 31.2 times 24.5 (24.3–36.5 times 20.0–27.6) μm with a bilayered wall about 1.7 μm thick. The micropyle was covered by a dome-shaped cap. The oocyst residuum was absent, but tiny polar granules were present. The sporocysts were elongate ovoid, 14.3 times 7.2 (11.5–18.5 times 6.0–9.0) μm, had a Stieda body, but lacked a substiedal body. The sporocyst residuum was present, composed of numerous small granules. The sporozoites were elongate club-shaped, and contained two prominent refractile bodies. 相似文献
49.
50.
Possible protective mechanisms exerted by metformin or metformin and vitamin E in isoproterenol‐induced cardiac injury 下载免费PDF全文