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61.
A L Okorokov N O Bukanov O Iu Beskrovnaia E B Voroshilova M M Gusiatiner V G Gritsenko N K Iankovski? V G Debabov 《Genetika》1990,26(4):648-656
Novel cloning vectors for glutamic acid producing bacteria have been constructed. The cryptic plasmid pBO1 (4.4 kb) from Brevibacterium sp. recombined with the plasmid pACYC184 (4.0 kb) from Escherichia coli was used to produce composite plasmid named pKA1. The plasmid could propagate and express the Cm-r phenotype in E. coli and coryneform glutamic acid producing bacteria Br. flavum, C. glutamicum, Br. lactofermentum. The pKA1 plasmid and its variants deleted within non-essential plasmid regions with unique restriction sites HindIII, SalGI, SphI were used in cloning experiments. The genes coding for threonine biosynthesis of C. glutamicum and Br. flavum were subcloned into shuttle vectors in C. glutamicum cells. Recombinant plasmids were introduced into protoplasts by polyethylenglycol-mediated transformation of plasmid DNAs. It was shown that the presence of plasmids containing the Br. flavum thrA2 gene in C. glutamicum (thrB) caused 10-fold increase in homoserine dehydrogenase activity, as compared to that of wild type strain, and in homoserine production. 相似文献
62.
Content of free and bound thiamine diphosphate in the liver hyaloplasm of vitamine B1 deficient rats
Iu M Ostrovski? A I Voskoboev E A Gritsenko V V Grushnik 《Prikladnaia biokhimiia i mikrobiologiia》1979,15(5):728-734
The amount of free and protein-bound thiamin diphosphate (TDP) in the liver hyaloplasm of B1 vitamin deficient rats has been measured. In the norm the content of protein-bound TDP remains stable (4.5--4.7 micrograms/g tissue) and does not grow upon thiamin injections. The level of the free coenzyme varies appreciably: in the B1-avitaminotic state the content of free TDP decreases, and in the B1-saturated condition it may exceed the norm 4 times. In the liver this enzyme occurs only as a holoenzyme. In case of B1 vitamin deficiency in the diet the transketolase apoform cannot be detected in the liver. A new model for rapid generation of B1-avitaminosis characterized by a significantly lower level of free and bound TDP is described. 相似文献
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64.
S. V. Murzaeva M. B. Abramova I. I. Popova E. N. Gritsenko G. D. Mironova E. I. Lezhnev 《Biophysics》2010,55(5):727-732
The effect of Hypoxen (HX) on bioenergetic processes in the mitochondria of heart and liver of rats connected with respiration,
generation of hydrogen peroxide and activity of ATP-sensitive K-channel (mitoKATP) has been studied. It is shown that HX in
the range of 0.05–10 μg/mL stimulates respiration, increases the coupling in the respiratory chain, and increases the formation
of H2O2 and energy-dependent swelling associated with potassium transport in mitochondria. HX removes the inhibitory effect of ATP
on the energy-dependent swelling of mitochondria and partially reduces the accumulation of H2O2 in the presence of ATP. The role of antihypoxic and antioxidant action of HX associated with the activation of mitoKATP is discussed. 相似文献
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67.
Strong coupling of plant and fungal community structure across western Amazonian rainforests 总被引:1,自引:0,他引:1
The Amazon basin harbors a diverse ecological community that has a critical role in the maintenance of the biosphere. Although plant and animal communities have received much attention, basic information is lacking for fungal or prokaryotic communities. This is despite the fact that recent ecological studies have suggested a prominent role for interactions with soil fungi in structuring the diversity and abundance of tropical rainforest trees. In this study, we characterize soil fungal communities across three major tropical forest types in the western Amazon basin (terra firme, seasonally flooded and white sand) using 454 pyrosequencing. Using these data, we examine the relationship between fungal diversity and tree species richness, and between fungal community composition and tree species composition, soil environment and spatial proximity. We find that the fungal community in these ecosystems is diverse, with high degrees of spatial variability related to forest type. We also find strong correlations between α- and β-diversity of soil fungi and trees. Both fungal and plant community β-diversity were also correlated with differences in environmental conditions. The correlation between plant and fungal richness was stronger in fungal lineages known for biotrophic strategies (for example, pathogens, mycorrhizas) compared with a lineage known primarily for saprotrophy (yeasts), suggesting that this coupling is, at least in part, due to direct plant–fungal interactions. These data provide a much-needed look at an understudied dimension of the biota in an important ecosystem and supports the hypothesis that fungal communities are involved in the regulation of tropical tree diversity. 相似文献
68.
The genetic origins of altruism, defined here as a costly act aimed to benefit non-kin individuals, have not been examined in young children. However, previous findings concerning adults pointed at the arginine vasopressin receptor 1A (AVPR1A) gene as a possible candidate. AVPR1A has been associated with a range of behaviors including aggressive, affiliative and altruistic phenotypes, and recently a specific allele (327 bp) of one of its promoter region polymorphisms (RS3) has been singled out in particular. We modeled altruistic behavior in preschoolers using a laboratory-based economic paradigm, a modified dictator game (DG), and tested for association between DG allocations and the RS3 "target allele." Using both population and family-based analyses we show a significant link between lower allocations and the RS3 "target allele," associating it, for the first time, with a lower proclivity toward altruistic behavior in children. This finding helps further the understanding of the intricate mechanisms underlying early altruistic behavior. 相似文献
69.
Lugovskoy EV Gritsenko PG Koshel TA Koliesnik IO Cherenok SO Kalchenko OI Kalchenko VI Komisarenko SV 《The FEBS journal》2011,278(8):1244-1251
Calix[4]arenes bearing two or four methylenebisphosphonic acid groups at the macrocyclic upper rim have been studied with respect to their effects on fibrin polymerization. The most potent inhibitor proved to be calix[4]arene tetrakis-methylene-bis-phosphonic acid (C-192), in which case the maximum rate of fibrin polymerization in the fibrinogen + thrombin reaction decreased by 50% at concentrations of 0.52 × 10(-6) M (IC(50)). At this concentration, the molar ratio of the compound to fibrinogen was 1.7 : 1. For the case of desAABB fibrin polymerization, the IC(50) was 1.26 × 10(-6) M at a molar ratio of C-192 to fibrin monomer of 4 : 1. Dipropoxycalix[4]arene bis-methylene-bis-phosphonic acid (C-98) inhibited fibrin desAABB polymerization with an IC(50) = 1.31 × 10(-4) M. We hypothesized that C-192 blocks fibrin formation by combining with polymerization site 'A' (Aα17-19), which ordinarily initiates protofibril formation in a 'knob-hole' manner. This suggestion was confirmed by an HPLC assay, which showed a host-guest inclusion complex of C-192 with the synthetic peptide Gly-Pro-Arg-Pro, an analogue of site 'A'. Further confirmation that the inhibitor was acting at the initial step of the reaction was obtained by electron microscopy, with no evidence of protofibril formation being evident. Calixarene C-192 also doubled both the prothrombin time and the activated partial thromboplastin time in normal human blood plasma at concentrations of 7.13 × 10(-5) M and 1.10 × 10(-5) M, respectively. These experiments demonstrate that C-192 is a specific inhibitor of fibrin polymerization and blood coagulation and can be used for the design of a new class of antithrombotic agents. 相似文献
70.
Angel TE Jacobs JM Spudich SS Gritsenko MA Fuchs D Liegler T Zetterberg H Camp DG Price RW Smith RD 《Clinical proteomics》2012,9(1):3-21