An automated,high‐throughput method for targeted quantification of intact insulin and its therapeutic analogs in human serum or plasma coupling mass spectrometric immunoassay with high resolution and accurate mass detection (MSIA‐HR/AM)

The detection and quantification of insulin and its therapeutic analogs is important for medical, sports doping, and forensic applications. Synthetic variants contain slight sequence variations to affect bioavailability. To reduce sample handling bias, a universal extraction method is required for simultaneous extraction of endogenous and variant insulins with subsequent targeted quantification by LC‐MS. A mass spectrometric immunoassay (MSIA), a multiplexed assay for intact insulin and its analogues that couples immunoenrichment with high resolution and accurate mass (HR/AM) spectrometric detection across the clinical range is presented in this report. The assay is sensitive, selective, semi‐automated and can potentially be applied to detect new insulin isoforms allowing their further incorporation into second or third generation assays.     

A new genus of the Gymnoascaceae with a rudimentary peridium

Summary A new genus of the Gymnoascaceae is described and illustrated. A single species,Petalosporus nodulosus, is described. Four isolates representing this new species were isolated from dung in India and California. Morphological development and other diagnostic characteristics are given. The new genus is characterized by the formation of a discrete but often fragmentary ascocarp the peridium of which undergoes partial disarticulation at maturity. At least some of the peridial hyphae have thick walls and are enlarged at septal nodes, and these septa are also the points of disarticulation. A primary generic character is the floral-shaped pattern in which ascospores are found.     

Chrysosporium tropicum as a probable cause of mycosis of poultry in India

Chrysosporium tropicum was isolated from comb lesions in two different breeds of chickens in India and subcultures were shown to be pathogenic when inoculated onto prepared skin of guinea pigs. This report provides additional evidence to considerCh. tropicum as a pathogenic fungus and a probable cause of a dermatomycosis in chickens.