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排序方式: 共有175条查询结果,搜索用时 31 毫秒
91.
Sidorov Vladimir Wang Dafu Nagy Ervin D. Armstrong Charles Beach Steven Zhang Yuanji Groat Jeanna Yang Sam Yang Peizhen Gilbertson Larry 《In vitro cellular & developmental biology. Plant》2022,58(3):447-456
In Vitro Cellular & Developmental Biology - Plant - Application of new genome editing technologies for plant improvement depends on efficient delivery of editing components into plant cells.... 相似文献
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93.
Ream TS Strobel J Roller B Auger DL Kato A Halbrook C Peters EM Theuri J Bauer MJ Addae P Dioh W Staub JM Gilbertson LA Birchler JA 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2005,111(2):378-385
A maize line expressing Cre recombinase as well as the recipient line without the transgene were assayed for evidence of ectopic recombination within the maize genome. Such a test is valuable for understanding the action of Cre as well as for its use to recombine two target lox sites present in the chromosomes. Pollen examination and seed set tests of material expressing Cre provided no evidence of ectopic recombination, which would be manifested in the production of translocations or inversions and result in pollen abortion and reduced seed set. Root-tip chromosome karyotype analysis was also performed on material with and without Cre expression. Chromosomal aberrations in Cre+ material were not observed above the background level. 相似文献
94.
A species of Labyrinthula that causes 'rapid blight' and death of turfgrass has been isolated and studied. We name this new species Labyrinthula terrestris and briefly summarize morphological characteristics and growth patterns of this pathogen of turfgrass. 相似文献
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DNA microarrays are being used to comprehensively examine gene expression networks during the plant defense response that
is triggered when a plant encounters a pathogen or an elicitor molecule. In addition to identifying new genes induced during
defense, these studies are providing new insights into the complex pathways governing defense gene regulation. 相似文献
98.
Is there an inner nose? 总被引:3,自引:3,他引:0
Although behavioral and neuropsychological data regarding the existence of
images for odors are inconclusive, reconsideration of earlier EEG work
provides reasonably clear evidence for an inner nose. However, further EEG
studies and neuroimaging data seem essential for conclusive demonstration
of an inner nose.
相似文献
99.
Xudong Ye Yurong Chen Yuechun Wan Yun-Jeong Hong Martin C. Ruebelt Larry A. Gilbertson 《Plant cell reports》2016,35(3):601-611
Key message
virG mutant strains of a nopaline type of Agrobacterium tumefaciens increase the transformation frequency in cotton meristem transformation. Constitutive cytokinin expression from the tzs gene in the virG mutant strains is responsible for the improvement.Abstract
Strains of Agrobacterium tumefaciens were tested for their ability to improve cotton meristem transformation frequency. Two disarmed A. tumefaciens nopaline strains with either a virGN54D constitutively active mutation or virGI77V hypersensitive induction mutation significantly increased the transformation frequency in a cotton meristem transformation system. The virG mutant strains resulted in greener explants after three days of co-culture in the presence of light, which could be attributed to a cytokinin effect of the mutants. A tzs knockout strain of virGI77V mutant showed more elongated, less green explants and decreased cotton transformation frequency, as compared to a wild type parental strain, suggesting that expression of the tzs gene is required for transformation frequency improvement in cotton meristem transformation. In vitro cytokinin levels in culture media were tenfold higher in the virGN54D strain, and approximately 30-fold higher in the virGI77V strain, in the absence of acetosyringone induction, compared to the wild type strain. The cytokinin level in the virGN54D strain is further increased upon acetosyringone induction, while the cytokinin level in the virGI77V mutant is decreased by induction, suggesting that different tzs gene expression regulation mechanisms are present in the two virG mutant strains. Based on these data, we suggest that the increased cytokinin levels play a major role in increasing Agrobacterium attachment and stimulating localized division of the attached plant cells.100.
Modulation of human rheumatoid factor-specific lymphocyte responses with a cross-reactive anti-idiotype bearing the internal image of antigen 总被引:4,自引:0,他引:4
S Fong T A Gilbertson P P Chen J H Vaughan D A Carson 《Journal of immunology (Baltimore, Md. : 1950)》1984,132(3):1183-1189
Rabbit anti-idiotypic antibodies to human rheumatoid factor (RF) autoantibodies were isolated by affinity chromatography on rabbit anti-human IgG Fc Sepharose 4B. The anti-idiotypic antibodies bore the "internal image" of the antigen, human IgG. They reacted specifically with multiple human monoclonal and polyclonal IgM-RF, independent of any particular light or heavy chain amino acid sequence. The anti-idiotypes did not react with IgM or IgG proteins lacking RF activity. The present experiments determined the potential of the "internal image" antibodies to modulate in vitro lymphocyte functions. The addition of anti-idiotypic antibody to peripheral blood mononuclear cell cultures from patients with rheumatoid arthritis elicited lymphocyte proliferation, but not RF synthesis. The antibody did not induce the proliferation of lymphocytes from a normal individual. Moreover, the anti-idiotype specifically suppressed IgM-RF secretory responses when preincubated with B cells before co-culture with autologous pokeweed mitogen-activated T cells. The data show that the anti-idiotypic antibodies with the "internal image" of antigen are capable of interacting with B cell receptors in an antigen-restricted manner, and possess specific immunomodulatory properties. 相似文献