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Gagan Dhawan Ramesh Chandra Kailash C. Gupta 《Nucleosides, nucleotides & nucleic acids》2015,34(3):149-162
In our previous report [Kumar, P.; Dhawan, G.; Chandra, R.; Gupta, K.C. Polyamine-assisted rapid and clean cleavage of oligonucleotides from cis-diol bearing universal support. Nucl. Acids Res. 2002, 30, e130 (1-8)], we demonstrated polyamine-mediated deprotection of oligonucleotides from cis-diol group bearing universal polymer support (I). However, vulnerability of the conventional dCbz to modifications under these conditions compelled us to employ dCac during synthesis of oligonucleotide using conventional synthons. Here, a new set of simple and rapid deprotection conditions has been developed for the complete cleavage of oligonucleotides from the 1,4-anhydroerythritol-based universal polymer support employing conventional dCbz synthon. Using manganese-imidazole complex in aqueous ammonium hydroxide (~30%), fully deprotected oligonucleotide sequences were obtained in 40 min, which were analyzed on reverse phase-HPLC and compared with the standard oligomers in terms of their retention time. Finally, their biological compatibility was established by analyzing PCR amplified products of npsA gene of N. meningitidis. 相似文献
213.
There are many applications where a timely response to an important event is needed. Often such response can require significant
computation and possibly communication, and it can be very challenging to complete it within the time-frame the response is
needed. At the same time, there could be application-specific flexibility in the computation that may be desired.
This paper presents the design, implementation, and evaluation of a middleware that can support such applications. Each of
the services in our target applications could have one or more service parameters, which can be modified, within the pre-specified
ranges, by the middleware. The middleware enables the time-critical event handling to achieve the maximum benefit, as per
the user-defined benefit function, while satisfying the time constraint. Our middleware is also based on the existing Grid
infrastructure and Service-Oriented Architecture (SOA) concepts. We have evaluated our middleware and its support for adaptation
using a volume rendering application and a Great Lake forecasting application. The evaluation shows that our adaptation is
effective, and has a very low overhead.
相似文献
Gagan AgrawalEmail: |
214.
Neonatal Persistent Exposure to 6‐Propyl‐2‐thiouracil,a Thyroid‐Disrupting Chemical,Differentially Modulates Expression of Hepatic Catalase and C/EBP‐β in Adult Rats 下载免费PDF全文
Suresh Kumar Bunker Jagneshwar Dandapat Sunil Kumar Sahoo Anita Roy Gagan B. N. Chainy 《Journal of biochemical and molecular toxicology》2016,30(2):80-90
Persistent exposure of rats to 6‐propyl‐2‐thiouracil (PTU) from birth resulted in decreases in plasma thyroid hormone (TH) levels and hepatic expression of catalase and CCAAT enhancer binding protein β (C/EBP‐β). Catalase promoter region (–185 to +52) that contains binding sites for C/EBP‐β showed an augmentation in the methylation level along with a change in methylation pattern of CpG islands in response to PTU treatment. PTU withdrawal on 30 days of birth restored TH levels and C/EBP‐β to control rats in adulthood. Although catalase expression was restored to some extent in adult rats in response to PTU withdrawal, a permanent change in its promoter CpG methylation pattern was recorded. The results suggest that downregulation of adult hepatic catalase gene in response to persistent neonatal PTU exposure may not solely be attributed to thyroid‐disrupting properties of PTU. It is possible that besides thyroid‐disrupting behavior, PTU may impair expression of hepatic catalase by altering methylation pattern of its promoter. 相似文献
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The present study investigates the antioxidative effects of vitamin E and curcumin against l-thyroxine (T4)-induced oxidative stress in renal cortex of adult male rats. Rats were made hyperthyroid by administration of l-thyroxine (0.0012%) in their drinking water for 30 days. Vitamin E (200 mg/kg body weight/day) and curcumin (30 mg/kg body
weight/day) were supplemented singly or in combination orally for 30 days along with l-thyroxine treatment. The elevated level of oxidative stress parameters (lipid peroxidation and protein carbonylation) and
decline level of small antioxidant molecules (reduced glutathione and ascorbic acid) in renal cortex of T4-treated rats were restored back by supplementation of vitamin E or/and curcumin. Increased superoxide dismutase and catalase
activities in kidney cortex of T4-treated rats were ameliorated in response to vitamin E or/and curcumin treatment. The elevated translated product of Cu/Zn-SOD,
Mn-SOD and catalase in T4-treated rats were differentially reduced by the administration of vitamin E and curcumin independently or in combination.
Cu/Zn-SOD expression was ameliorated by both vitamin E and curcumin independently or in combination, whereas Mn-SOD expression
was ameliorated by the supplementation of vitamin E or curcumin independently. However, the expression of catalase was alleviated
by only supplementation of vitamin E to T4-treated rats. The results suggest that both vitamin E and curcumin may play an important role in protecting T4-induced oxidative stress in rat renal cortex by differentially modulating the activities of antioxidant enzymes and oxidative
stress parameters. 相似文献
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Assessment of structural and functional similarity of biosimilar products: Rituximab as a case study
Biosimilars are products that are similar in terms of quality, safety, and efficacy to an already licensed reference/ innovator product and are expected to offer improved affordability. The most significant source of reduction in the cost of development of a biosimilar is the reduced clinical examination that it is expected to undergo as compared to the innovator product. However, this clinical relief is predicated on the assumption that there is analytical similarity between the biosimilar and the innovator product. As a result, establishing analytical similarity is arguably the most important step towards successful development of a biosimilar. Here, we present results from an analytical similarity exercise that was performed with five biosimilars of rituximab (Ristova®, Roche), a chimeric mouse/ human monoclonal antibody biotherapeutic, that are available on the Indian market. The results show that, while the biosimilars exhibited similarity with respect to protein structure and function, there were significant differences with respect to size heterogeneity, charge heterogeneity and glycosylation pattern. 相似文献