全文获取类型
收费全文 | 510935篇 |
免费 | 49919篇 |
国内免费 | 212篇 |
专业分类
561066篇 |
出版年
2018年 | 5752篇 |
2017年 | 5319篇 |
2016年 | 7368篇 |
2015年 | 10185篇 |
2014年 | 11314篇 |
2013年 | 16408篇 |
2012年 | 18466篇 |
2011年 | 18487篇 |
2010年 | 12358篇 |
2009年 | 10586篇 |
2008年 | 16113篇 |
2007年 | 16731篇 |
2006年 | 15516篇 |
2005年 | 14696篇 |
2004年 | 14532篇 |
2003年 | 13905篇 |
2002年 | 13421篇 |
2001年 | 18981篇 |
2000年 | 19000篇 |
1999年 | 15477篇 |
1998年 | 5638篇 |
1997年 | 5771篇 |
1996年 | 5451篇 |
1995年 | 5178篇 |
1994年 | 5096篇 |
1993年 | 4984篇 |
1992年 | 13219篇 |
1991年 | 12940篇 |
1990年 | 12872篇 |
1989年 | 12593篇 |
1988年 | 11849篇 |
1987年 | 11071篇 |
1986年 | 10334篇 |
1985年 | 10813篇 |
1984年 | 8945篇 |
1983年 | 7682篇 |
1982年 | 5939篇 |
1981年 | 5331篇 |
1980年 | 4977篇 |
1979年 | 8523篇 |
1978年 | 6658篇 |
1977年 | 6257篇 |
1976年 | 5971篇 |
1975年 | 6468篇 |
1974年 | 7041篇 |
1973年 | 6914篇 |
1972年 | 6370篇 |
1971年 | 5775篇 |
1970年 | 4999篇 |
1969年 | 5001篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
951.
A number of motile strains of Azospirillum brasilense, A. lipoferum, and A. irakense, were found to possess tyrosinase activity both on the surface of and inside the cells. A. brasilense Sp245, Sp7, and A. irakense KBC-1 each possessed two forms of tyrosinase of different molecular masses; A. lipoferum 43, A. lipoferum 59b, and A. irakense KA-3 each had a single tyrosinase form of approximately the same molecular mass; and A. brasilense Sp107 possessed a single form of tyrosinase different from all the other forms. 相似文献
952.
M G Netsky 《BMJ (Clinical research ed.)》1986,293(6562):1670-1672
953.
Effect of carbon source on lysine-mediated inhibition of postexponential growth of Saccharomyces cerevisiae 总被引:1,自引:0,他引:1 下载免费PDF全文
T G Watson 《Journal of bacteriology》1983,154(2):1013-1014
Lysine-mediated inhibition of postexponential growth in Saccharomyces cerevisiae occurred when glucose, fructose, or maltose, but not lactate, pyruvate, or ethanol, was used as the carbon source. Arginine starvation is not responsible for the inhibitory effect, since neither the intracellular pool of glucose-grown (inhibited) cells nor that of lactate-grown (noninhibited) cells contained arginine. 相似文献
954.
K M Le B L Madsen P W Barth G A Ksander J B Angell L M Vistnes 《Plastic and reconstructive surgery》1984,74(6):745-756
Three-dimensional scalar pressure distributions were measured in solid tissue near bony prominences in vitro in meat and in vivo in pigs using silicon pressure sensors. Data are in accord with previous theoretical models and indicate that pressure is three to five times higher internally near a bony prominence than it is at the skin over the prominence. Pressure sores are thus thought to begin internally; by the time they are evident at the skin, the sore has worked its way completely from bone to skin. This conclusion is in accord with previous clinical data. Future measurement of local vector forces is needed to fully characterize the force distribution in vivo. 相似文献
955.
A Santagostino G Giagnoni P Fumagalli D Pavesi E Torretta 《Biochemical and biophysical research communications》1982,104(2):577-582
The isoelectric point of the camel and the human β-endorphin, of the α-endorphin and the enkephalins were determined by analytical isoelectric focusing on 1 mm thin polyacrylamide gel slab. The difficulty of staining peptides as short as β-endorphin or smaller was overcomed using a modification of Bibring and Baxandall's or Faupel and Von Arx's staining method. The camel β-endorphin gives two bands having isoelectric point of 10.3 and 10.4, the human β-endorphin focus at pH 9.9, while α-endorphin, leu and met-enkephalin at pH 5.9, 5.5 and 5.45 respectively. The staining method described coupled with the isoelectric focusing seems to be fit for discriminating β-endorphin in a crude rat pituitary extract. 相似文献
956.
We have investigated the effects of two 4-ene-steroid 5 alpha-reductase inhibitors, diethyl-4-methyl-3-oxo-4-aza-5 alpha-androstane-17 beta-carboxamide (4-MA) and (4R)-5,10-seco-19-norpregna-4, 5-diene-3,10,20-trione (SECO), on testicular and epididymal androgen biosynthesis. Kinetic analyses revealed that both compounds inhibited epididymal DHT biosynthesis. 4-MA was a competitive inhibitor of epididymal nuclear and microsomal 4-ene-steroid 5 alpha-reductases (3-oxo-5 alpha-steroid: NADP 4-ene-oxidoreductase EC 1.3.1.22) with Kiapp values of 12.8 and 15.1 nmol/l compared to the respective Kmapp values of 185 and 240 nmol/l. Values for the Vmaxapp were always within 70-130% of the control. SECO at 1.0 mumol/l, also inhibited epididymal nuclear and microsomal 4-ene-steroid-5 alpha-reductases, causing respectively 2.9 and 5.2-fold increases in Kmapp. The Vmaxapp values were unchanged. However, SECO concentrations of 5 and 25 mumol/l abolished 4-ene-steroid 5 alpha-reductase activity at all testosterone concentrations. To examine the specificity of these compounds, we investigated their effects on the enzymes that convert pregnenolone to testosterone. Rat testis microsomes converted pregnenolone to testosterone via the 4-ene-3-oxo pathway, with the major metabolites being progesterone, 17-hydroxyprogesterone, 4-androstenedione and testosterone; some 17-hydroxypregnenolone was also formed. Very small amounts of dehydroepiandrosterone (DHA) and 5-androstenediol were detected. SECO, at a concentration that completely inhibited epididymal 4-ene-steroid 5 alpha-reductase activity, did not alter the metabolic profile of pregnenolone metabolism. However, 4-MA prevented the appearance of 4-ene steroids, and large quantities of 17-hydroxypregnenolone and DHA accumulated, suggesting that inhibition of the 3 beta-hydroxysteroid: NAD(P)+ oxidoreductase (EC 1.1.1.51) and 3-oxosteroid 5-ene-4-ene-isomerase (EC 5.3.3.1) [3 beta-hydroxysteroid dehydrogenase-isomerase] was occurring. Optimal conditions for the microsomal conversion of DHA to 4-androstenedione were determined; kinetic analyses of the 3 beta-hydroxysteroid dehydrogenase-isomerase activity revealed that 4-MA inhibited this reaction non-competitively, reducing Vmaxapp values to 25% of the control. The Kiapp determined from the intercept replot, was 121 nmol/l, and the Kmapp was always between 90 and 130% of the control value. It is concluded that SECO is more specific than 4-MA in its effects on androgen biosynthesis in the testis and epididymis and that both these drugs should provide useful tools in assessments of the relative contributions of 5 alpha-reduced androgens to androgen dependent processes. 相似文献
957.
958.
A common step in prokaryotic recombination appears to be the synapsis of the 3'-end of single-stranded DNA with duplex DNA to form a D-loop. The enzymatic mechanisms by which 3'-ends are produced and by which D-loops are converted into recombinant molecules are illustrated by proposed mechanisms of recombination by the Escherichia coli RecBCD pathway and the phage lambda Red pathway. The enzymes promoting recombination and the special DNA sites at which they act are emphasized. Recombination by other E. coli pathways and in other prokaryotes is compared with these mechanisms. 相似文献
959.
960.