全文获取类型
收费全文 | 529篇 |
免费 | 45篇 |
国内免费 | 1篇 |
出版年
2024年 | 1篇 |
2023年 | 5篇 |
2022年 | 5篇 |
2021年 | 16篇 |
2020年 | 15篇 |
2019年 | 16篇 |
2018年 | 18篇 |
2017年 | 15篇 |
2016年 | 15篇 |
2015年 | 34篇 |
2014年 | 44篇 |
2013年 | 45篇 |
2012年 | 43篇 |
2011年 | 54篇 |
2010年 | 37篇 |
2009年 | 28篇 |
2008年 | 37篇 |
2007年 | 17篇 |
2006年 | 27篇 |
2005年 | 14篇 |
2004年 | 21篇 |
2003年 | 17篇 |
2002年 | 15篇 |
2001年 | 9篇 |
2000年 | 1篇 |
1999年 | 2篇 |
1998年 | 2篇 |
1997年 | 2篇 |
1996年 | 1篇 |
1995年 | 1篇 |
1993年 | 3篇 |
1992年 | 4篇 |
1991年 | 2篇 |
1988年 | 1篇 |
1987年 | 1篇 |
1979年 | 1篇 |
1977年 | 1篇 |
1974年 | 3篇 |
1957年 | 2篇 |
排序方式: 共有575条查询结果,搜索用时 15 毫秒
571.
Mammal Research - The Mazama genus is one of the more diverse genera among deer (Cervidae); its classification is controversial because of its complex phylogenetic relationships. The Central... 相似文献
572.
Circulating Cell-Free DNA from Colorectal Cancer Patients May Reveal High KRAS or BRAF Mutation Load
Florent Mouliere Safia El Messaoudi Celine Gongora Anne-Sophie Guedj Bruno Robert Maguy Del Rio Franck Molina Pierre-Jean Lamy Evelyne Lopez-Crapez Muriel Mathonnet Marc Ychou Denis Pezet Alain R Thierry 《Translational oncology》2013,6(3):319-IN8
Our data might suggest a very high variation in the proportion of malignant cells in colorectal tumors and/or low tumor cell clonogenicity. However, the direct relationship between these data and the proportion of malignant cells is currently uncertain as the mechanism of ccfDNA release determines the yield (necrosis, apoptosis, or active release). Therefore, interindividual comparison of the proportion of mutant allele determined from circulating DNA could appear somewhat more difficult to interpret; however, this parameter could be an efficient biomarker for monitoring or following up CRC patients and could be combined with size fraction analysis, as demonstrated in this study, to provide a deeper examination toward this goal. 相似文献
573.
Antje Pohl Iván López-Montero Antje Pohl Iván López-Montero Florent Rouvière Fabrice Giusti 《Molecular membrane biology》2013,30(3):194-204
In order to study the basic physical phenomena underlying complex lipid transbilayer movement in biological membranes, we have measured the transmembrane diffusion of spin-labelled analogues of sphingolipids in phosphatidylcholine (PC) large unilamellar vesicles in the absence or presence of cholesterol, going from a fluid ( liquid disordered) ld, phase to a more viscous, liquid ordered (lo), phase. We have found cholesterol to reduce the transverse diffusion of glucosylceramide (GlcCer) and galactosylceramide (GalCer) in a concentration-dependent manner. However, surprisingly, we could neither detect any influence of cholesterol on the rapid flip-flop of ceramide nor on the flip-flop of dihydroceramide, for which the τ1/2 of flip-flop remains in the order of 1 minute at 20°C in the presence of cholesterol. As a consequence of rapid flip-flop of ceramide in both the lo and the ld phase, ceramide is likely to distribute between the two monolayers of a membrane, and could in principle partition into segregated domains in each side of the plasma membrane of eukaryotic cells. 相似文献
574.
Yan Li Florent Barbault Michel Delamar Ruisheng Zhang Rongjing Hu 《Journal of molecular modeling》2013,19(4):1651-1666
Some recent papers clearly indicate that the cytoplasmic domain of KcsA plays a role in pH sensing. We have performed, for the first time, a targeted molecular dynamics (TMD) simulation of the opening of full-length KcsA at pH 4 and pH 7, with a special interest for the cytoplasmic domain. Association energy calculations show a stabilization at pH 7 confirming that the protonation of some amino-acids at pH 4 in this domain plays a role in the opening process. A careful analysis of the pH dependent charges borne by residues in the cytoplasmic domain and their interactions confirms some literature experimental data and permits to give further insight into the role played by some of them in the opening process. 相似文献
575.
Florent Angly Merry Youle Bahador Nosrat Shailaja Srinagesh Beltran Rodriguez-Brito Patrick McNairnie Gordafaried Deyanat-Yazdi Mya Breitbart Forest Rohwer 《Environmental microbiology》2009,11(11):2863-2873
Roseophage SIO1 is a lytic marine phage that infects Roseobacter SIO67, a member of the Roseobacter clade of near-shore alphaproteobacteria. Roseophage SIO1 was first isolated in 1989 and sequenced in 2000. We have re-sequenced and re-annotated the original isolate. Our current annotation could only assign functions to seven additional open reading frames, indicating that, despite the advances in bioinformatics tools and increased genomic resources, we are still far from being able to translate phage genomic sequences into biological functions. In 2001, we isolated four new strains of Roseophage SIO1 from California near-shore locations. The genomes of all four were sequenced and compared against the original Roseophage SIO1 isolated in 1989. A high degree of conservation was evident across all five genomes; comparisons at the nucleotide level yielded an average 97% identity. The observed differences were clustered in protein-encoding regions and were mostly synonymous. The one strain that was found to possess an expanded host range also showed notable changes in putative tail protein-coding regions. Despite the possibly rapid evolution of phage and the mostly uncharacterized diversity found in viral metagenomic data sets, these findings indicate that viral genomes such as the genome of SIO1-like Roseophages can be stably maintained over ecologically significant time and distance (i.e. over a decade and ∼50 km). 相似文献