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141.
142.
Christoph Koffler Jon Dettling Cashion East Matthias Finkbeiner Sergio F. Galeano Roland Geyer Mark J. Goedkoop Troy R. Hawkins Connie D. Hensler Arpad Horvath Sebastien Humbert Scott M. Kaufman Amy E. Landis Lise Laurin Pascal Lesage Manuele Margni Ken Martchek H. Scott Matthews Jamie K. Meil Gregory Norris Rita C. Schenk Thomas P. Seager Maureen Sertich Greg Thoma Casey Wagner 《The International Journal of Life Cycle Assessment》2013,18(2):302-305
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Establishment and characterization of a cell line derived from bovine tracheal glands 总被引:3,自引:0,他引:3
W. E. Finkbeiner J. A. Nadel C. B. Basbaum 《In vitro cellular & developmental biology. Plant》1986,22(10):561-567
Summary Bovine tracheal submucosal gland cells have been isolated by enzymatic digestion and serially propagated in tissue culture
for more than 12 mo. (40 passages). The cells exhibit an epithelioid appearance at confluence and contain alcian blue (pH
2.5)/periodic acid-Schiff-positive material within cytoplasmic granules. By electron microscopy numerous osmiophilic secretory
granules are seen. Maximal growth is observed when the cells are grown on human placental collagen-coated culture vessels
in medium supplemented with 20% fetal bovine serum. Scintillation spectrometry revealed that radiolabeled precursor (35SO4) was incorporated into high molecular weight molecules and released from cells. Isoproterenol (10−6 to 10−3
M) stimulated the release of35SO4. The maximal response to isoproterenol was completely inhibited by the β-adrenergic antagonist propranolol. It is concluded
that the cultured cells retain features of tracheal gland cells and may serve as a useful model of synthesis and secretion
of macromolecules by tracheal gland cells.
This study was supported in part by NIH Program Project grant HL-24136, by a National Cystic Fibrosis Foundation Research
Development Grant, and by a grant from Cystic Fibrosis Research, Inc. Dr. Finkbeiner is a recipient of NIH Clinical Investigator
Award HL-01387. 相似文献
145.
Steven M. Finkbeiner 《The Yale journal of biology and medicine》1987,60(6):601-Dec;60(6):601
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Airway secretion: a cell-specific analysis 总被引:1,自引:0,他引:1
Respiratory mucus is a complex secretion elaborated by several cell types in the airway wall: serous and mucous gland cells in the submucosa, and ciliated and goblet cells in the epithelium. The cell types are under independent regulatory mechanisms, most of which are poorly understood. Two new approaches are described which permit analysis of these mechanisms and the role of each cell type in mucus production. Using cell-specific monoclonal antibodies, we have obtained biochemical information about mucus components contributed by the various cell types. Antibodies are also being used in enzyme immunoassays (ELISA) to detect cell-specific secretion from mixed cell biopsies. In other studies, analysis of secretory products from pure cultures of serous gland cells has revealed that the major glycoconjugates released by this cell type are chondroitin sulfate proteoglycans, hyaluronic acid and N-linked glycoproteins of complex type. 相似文献
148.
M. Yanina Pepino Susana Finkbeiner Julie A. Mennella 《Obesity (Silver Spring, Md.)》2009,17(6):1158-1163
The present study assessed food cravings in a cohort of 229 women who differed in smoking history (i.e., never smoker, former smoker, and current smoker) and body weight (i.e., normal weight, overweight, and obese). Each subject completed the Food Craving Inventory (FCI), which measures cravings for sweets, high fats, carbohydrates/starches, and fast‐food fats, and the Profile of Mood States (POMS), which measures psychological distress. Smoking and obesity were independently associated with specific food cravings and mood states. Current smokers craved high fats more frequently than former and never smokers. They also craved starches more frequently and felt more depressed and angry than never smokers, but not former smokers. Whereas cravings for starchy foods and some mood states may be characteristic of women who are likely to smoke, more frequent cravings for fat among smokers is related to smoking per se. Similarly, obese women craved high fats more frequently than nonobese women and depression symptoms were intensified with increasing body weights. We hypothesize that the overlapping neuroendocrine alterations associated with obesity and smoking and the remarkable similarities in food cravings and mood states between women who smoke and women who are obese suggest that common biological mechanisms modulate cravings for fat in these women. 相似文献
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