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101.
Thomas?Fett Laurent?LM?Zecchinon Etienne?A?Baise Daniel?JM?DesmechtEmail author 《BMC veterinary research》2005,1(1):4
Background
Lymphocyte function-associated antigen-1 (LFA-1, CD11a/CD18, alpha L beta 2) is required for many cellular adhesive interactions during the immune response.Results
The Capra hircus CD11a-encoding cDNA was sequenced and compared with its human, murine, rat, bovine and ovine counterparts. Despite some focal differences, it shares all the main characteristics of its known mammalian homologues.Conclusion
Therefore, along with the caprine CD18-encoding cDNA, which has been available for a few months, the sequence data revealed here will allow the Capra hircus LFA-1 expression in vitro as a tool to explore the specificities of inflammation in the caprine species.102.
Robert A. Moreau Michael J. Powell William F. Fett Bruce D. Whitaker 《Current microbiology》1997,35(2):124-128
Zymomonas mobilis (ATCC 29191) was grown either aerobically or anaerobically in the presence of 2% (wt/vol) glucose and 0, 3, or 6% (vol/vol)
ethanol. The rates of growth and the composition of hopanoids, cellular fatty acids, and other lipids in the bacterial membranes
were quantitatively analyzed. The bacterium grew in the presence of 3% and 6% ethanol and was more ethanol tolerant when grown
anaerobically. In the absence of ethanol, hopanoids comprised about 30% (by mass) of the total cellular lipids. Addition of
ethanol to the media caused complex changes in the levels of hopanoids and other lipids. However, there was not a significant
increase in any of the hopanoid lipid classes as ethanol concentration was increased. As previously reported, vaccenic acid
was the most abundant fatty acid in the lipids of Z. mobilis, and its high constitutive levels were unaffected by the variations in ethanol and oxygen concentrations. A cyclopropane
fatty acid accounted for 2.6–6.4 wt % of the total fatty acids in all treatments.
Received: 12 November 1996 / Accepted: 25 February 1997 相似文献
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109.
Exopolysaccharide production by Bifidobacterium longum BB-79 总被引:1,自引:0,他引:1
Cynthia M. Roberts W.F. Fett S.F. Osman C. Wijey J.V. O'Connor D.G. Hoover 《Journal of applied microbiology》1995,78(5):463-468
Bifidobacterium longum BB-79 produced an acidic extracellular polysaccharide (EPS), especially when grown on solid medium. The EPS was isolated by ethanol precipitation followed by dialysis and lyophilization. Anion exchange and gel-filtration chromatography were used to further purify and characterize the EPS. The average molecular weight was greater than 200 kDa as estimated by chromatography. Based on gas-liquid chromatography (GLC) and GLC-mass spectrometry analyses, the EPS appears to be composed of galactose and an unidentified hexose (possibly glucose) with a carboxyethyl (lactic acid) substituent. Lactose, when used as the primary carbon source in liquid media, gave the highest yield of EPS. Incubation times longer than 24 h and the initial culture pH (pH 6·0–9·0) had little effect on the amount of EPS produced. 相似文献
110.
Lysates of two established human tumor lines contain heparin-binding growth factors related to bovine acidic brain fibroblast growth factor 总被引:4,自引:0,他引:4
R R Lobb S M Rybak D K St Clair J W Fett 《Biochemical and biophysical research communications》1986,139(3):861-867
Cell lysates of two established human tumor lines, a medulloblastoma (TE671), and a rhabdomyosarcoma (RD), contain mitogenic activity which elutes from heparin-Sepharose under conditions typical of class 1 heparin-binding growth factors, such as acidic brain fibroblast growth factor. The presence of this class of mitogen in both cell lines was confirmed by their chromatographic behavior on reversed-phase C3 columns, and by the ability of heparin to enhance their mitogenic activity. Using a specific synthetic DNA probe, RNA's were isolated from both cell lines by hybridization-selection, translated in vitro, and translated proteins affinity fractionated on heparin-Sepharose. The results demonstrate that TE671 and RD cell lysates contain mRNA's for mitogens related to acidic brain fibroblast growth factor, and also suggest that high molecular weight proteins exist that are closely related to, or are precursor forms of, the class 1 mitogens. 相似文献