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A strategy for high cell density culture of heterotrophic microalgae with inhibitory substrates 总被引:5,自引:0,他引:5
Substrate inhibition is one of the major problems preventing high cell densities of microalgae in heterotrophic culture, so
the possibility of overcoming the problem by various culture techniques was examined. It was found that perfusion culture
may be the most appropriate technique for high cell densities in heterotrophic culture using inhibitory substrates. An experimental
example in which a hollow fibre cell recycle system (HFCRS) was employed to achieve high cell densities of Chlamydomonas reinhardtii on acetate under heterotrophic conditions of growth was demonstrated. The cell density in the HFCRS was much higher than
that reported in the literature for this species. 相似文献
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Social responsibility links personal behavior with societal expectations and plays a key role in affecting an agent’s emotional state following a decision. However, the neural basis of responsibility attribution remains unclear. In two previous event-related brain potential (ERP) studies we found that personal responsibility modulated outcome evaluation in gambling tasks. Here we conducted a functional magnetic resonance imaging (fMRI) study to identify particular brain regions that mediate responsibility attribution. In a context involving team cooperation, participants completed a task with their teammates and on each trial received feedback about team success and individual success sequentially. We found that brain activity differed between conditions involving team success vs. team failure. Further, different brain regions were associated with reinforcement of behavior by social praise vs. monetary reward. Specifically, right temporoparietal junction (RTPJ) was associated with social pride whereas dorsal striatum and dorsal anterior cingulate cortex (ACC) were related to reinforcement of behaviors leading to personal gain. The present study provides evidence that the RTPJ is an important region for determining whether self-generated behaviors are deserving of praise in a social context. 相似文献
85.
基于MODIS NDVI的长江中游区域植被动态及与气候因子的关系 总被引:1,自引:0,他引:1
基于1999-2015年的MODIS NDVI时间序列遥感数据,应用趋势分析、变异系数、重标极差分析和偏相关分析等方法,分析了长江中游的植被时空变化特征及其与气象因子的关系。结果表明,长江中游地区NDVI均值总体上呈上升趋势(从0.72增加到0.80)。从空间分布来看,NDVI低值区域(0.1-0.5)占1.40%,高值区域(>0.7)占87.15%;NDVI空间格局呈"西高东低、北高南低"的分布特征,低值区域表现为以三省省会城市为中心向外辐射。Hurst指数显示,研究区大部分区域(60.54%)的NDVI变化趋势具有不确定性,持续性改善区域(34.78%)主要分布在西部山地区,持续性退化区域(3.26%)主要分布在人类活动频繁的较发达城市区域。在年际尺度上,研究区NDVI与各气象因子关系均不显著;月际尺度上,NDVI与降水、相对湿度和日照时数显著相关,降水和日照时数有明显的时滞性。区域内NDVI动态趋势以不确定性发展为主,城市群周边NDVI呈现持续退化的区域应该引起关注。 相似文献
86.
分泌型磷脂酶PLA2G5属于磷脂酶A2超家族的一员,在免疫细胞和非免疫细胞中均有表达.研究表明,PLA2G5参与生物学事件的发生发展,在特定的病理条件下具有诱导作用.本文简要阐述了PLA2G5的来源、结构特征、生物学功能和在疾病中的作用,以及现有或潜在的PLA2G5抑制剂,以期探索基于PLA2G5的治疗新靶标. 相似文献
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Mengnan Wang Dongjie Li Mingyue Zhang Wenzhi Yang Yali Cui Shijie Li 《Animal genetics》2015,46(4):354-360
The CDKN1C gene encodes a cyclin‐dependent kinase inhibitor and is one of the key genes involved in the development of Beckwith–Wiedemann syndrome and cancer. In this study, using a direct sequencing approach based on a single nucleotide polymorphism (SNP) at genomic DNA and cDNA levels, we show that CDKN1C exhibits monoallelic expression in all seven studied organs (heart, liver, spleen, lung, kidney, muscle and subcutaneous fat) in cattle. To investigate how methylation regulates imprinting of CDKN1C in cattle, allele‐specific methylation patterns in two putative differential methylation regions (DMRs), the CDKN1C DMR and KvDMR1, were analyzed in three tissues (liver, spleen and lung) using bisulfite sequencing PCR. Our results show that in the CDKN1C DMR both parental alleles were unmethylated in all three analyzed tissues. In contrast, KvDMR1 was differentially methylated between the two parental alleles in the same tissues. Statistical analysis showed that there is a significant difference in the methylation level between the two parental alleles (P < 0.01), confirming that this region is the DMR of KvDMR1 and that it may be correlated with CDKN1C imprinting. 相似文献
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