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61.
Nunes OC Manaia CM Da Costa MS Santos H 《Applied and environmental microbiology》1995,61(6):2351-2357
(sup13)C nuclear magnetic resonance spectroscopy and (sup1)H nuclear magnetic resonance spectroscopy were used to identify and quantify the organic solutes of several strains of halophilic or halotolerant thermophilic bacteria. Two strains of Rhodothermus marinus and four strains of "Thermus thermophilus" grown in complex medium containing NaCl were examined. 2-O-Mannosylglycerate was a major compatible solute in all strains: the Thermus strains accumulated the (beta)-anomer only, whereas both anomers were found in R. marinus. 2-O-(beta)-mannosylglycerate and 2-O-(alpha)-mannosylglycerate were the major compatible solutes in R. marinus. The former was the predominant solute in cells grown in 2.0 and 4.0% NaCl-containing medium, while the latter was the predominant compatible solute at higher salinities. Glutamate, trehalose, and glucose were also present as minor components. The intracellular K(sup+) concentration, as determined by (sup39)K nuclear magnetic resonance spectroscopy, in R. marinus increased with salinity and was sufficient to balance the negative charges of the mannosylglycerate. In addition to 2-O-(beta)-mannosylglycerate, trehalose was a major compatible solute of "T. thermophilus." 2-O-(beta)-Mannosylglycerate was the main solute in medium containing 1.0 or 2.0% NaCl, while trehalose predominated in cells grown in medium supplemented with 3.0 or 4.0% NaCl. Glycine betaine, in lower concentrations, was also detected in two "T. thermophilus" strains. This is the first report of mannosylglycerate as a compatible solute in bacteria. 相似文献
62.
Juventino J. Garcia María L. Hernndez Hugo Torrens Atilano Gutierrez Federico del Río 《Inorganica chimica acta》1995,230(1-2):173-176
By reacting [(C5Me5)M(SRF)2] (forM = Ir, Rf = C6F5 (1a) or C6F4H-p (1b); for M = Rh, Rf = C6F5 (2a) or C6F4H-p (2a)) in toluene with Na[AuCl4], ionic binuclear compounds with the general formula [(C5Me5)M(μ-SRF)2AuCl2]Cl for M = Ir, R = C6F5 (3a) or C6F4H-p (3a); for M = Rh, RF = C6F5 (4a) or C6F4H-p (4b) can be obtained, together with small amounts of [(C5Me5)2Rh2(μ-SRF)(μ-Cl)2]Cl (RF = C6F5 (5a) or C6F4H-p (5b)) as by-products when 2a and 2b were used. 相似文献
63.
Bret B. Wagenhorst Ramji R. Rajendran Ellen E. Van Niel Richard B. Hessler Amir Bukelman Federico Gonzalez-Fernandez 《Journal of molecular evolution》1995,41(5):646-656
Vitamin A and fatty acids are critical to photoreceptor structure, function, and development. The transport of these nutrients between the pigment epithelium and neural retina is mediated by interphotoreceptor retinoid-binding protein (IRBP). IRBP, a 133-kDa (human) glycolipoprotein, is the major protein component of the extracellular matrix separating these two cell layers. In amphibians and mammals, IRBP consists of four homologous repeats of about 300 amino acids which form two retinol and four fatty acid-binding sites. Here we show that IRBP in teleosts is a simpler protein composed of only two repeats. Western blot analysis shows that goldfish IRBP is half the size (70 kDa) of IRBP in higher vertebrates. Metabolic labeling studies employing Brefeldin A taken together with in situ hybridization studies and the presence of a signal peptide show that goldfish IRBP is secreted by the cone photoreceptors. The translated amino acid sequence has a calculated molecular weight of 66.7 kDa. The primary structure consists of only two homologous repeats with a similarity score of 52.5%. The last repeats of human and goldfish IRBPs are 69.1% similar with hydrophobic regions being the most similar. These data suggest that two repeats were lost during the evolution of the ray-finned fish (Actinopterygii), or that the IRBP gene duplicated between the emergence of bony fish (Osteichthyes) and amphibians. Acquisition of a multirepeat structure may reflect evolutionary pressure to efficiently transport higher levels of hydrophobic molecules within a finite space. Quadruplication of an ancestral IRBP gene may have been an important event in the evolution of photoreceptors in higher vertebrates.
Correspondence to: F. Gonzalez-Fernandez 相似文献
64.
Alessandro Sisto Fabio Bonelli Felice Centini Christopher I. Fincham Edoardo Potier Edith Monteagudo Paolo Lombardi Federico Arcamone Cristina Goso Stefano Manzini Alessandro Giolitti Carlo A. Maggi Mariano Venanzi Basilio Pispisa 《Biopolymers》1995,36(4):511-524
In the course of a program aimed at synthesizing novel, potent NK-1 tachykinin receptor antagonists, we developed upon a bioactive model by comparing the low energy structures of a series of peptide and nonpeptide Substance P antagonists. The comparison was based on the super imposition of the aromatic rings, assuming that the rest of the molecule behaves predominantly as a template to arrange the key aromatic groups in the right spatial position. A series of 2-aminocyclohexane carboxylic acid analogues were then selected as the best templates for reproducing the postulated bioactive structure, leading to several pseudo-peptides with interesting biological activity. According to the molecular modeling, these compounds exhibit a neat parallel facing of the indolyl and naphthyl groups at about 3 Å distance. Ultraviolet absorption and steady state fluorescence measurements support this conclusion, showing a linear correlation between the spectral properties and the binding affinity of these analogues. Stacking of the indole ring with naphthalene gives rise to a complex characterized by a well-defined molar extinction coefficient. Consistently, steady state and lifetime fluorescence measurements suggest that the quenching process is ascribable to ground-state interactions between the chromophores. Implications of the π stacking propensity of aromatic groups in the biological activity of the compounds examined are briefly discussed. © 1995 John Wiley & Sons, Inc. 相似文献
65.
66.
Rodrigues C.R. Barreiro E.J. Romeiro N.C. Albuquerque M.G. De Sant'anna C.M.R. Bicca De Alencastro R. Da Motta Neto J.D. 《Molecular Engineering》1997,7(3-4):473-490
Two families of autacoids from cell membrane phospholipids have been identified. The first, the icosanoids, which are formed
from arachidonic acid, include prostaglandins and leukotrienes. The other includes modified phospholipids, as the platelet
aggregating factor (PAF). These compounds are related to inflammatory and cardiovascular diseases.
We review in this paper some of the work that has been done in our laboratories in the last few years relating to the modeling
of new potential thromboxane synthase (TXS) and 5-lipoxygenase (5-LO) and cyclooxygenase (COX) inhibitors, and TXA2 receptor antagonists derived from nitrogenated heterocycles. We include the results of the modeling of a group of proposed
PAF antagonists, and compare their structures with PAF itself and with a recently proposed PAF antagonist model.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献
67.
De Andrade Antônio V. M. Da Costa Nivan B. Longo Ricardo L. Malta Oscar L. Simas Alfredo M. De Sá Gilberto F. 《Molecular Engineering》1997,7(3-4):293-308
Theoretical techniques have been developed and/or improved to predict the molecular structure of lanthanide complexes which
were used to calculate their electronic properties, in particular, their electronic spectra and energy levels necessary to
calculate the rates of energy transfer from the ligands to the metal ion. The molecular structure has been obtained by the
SMLC/AM1 (Sparkle Model for the Calculation of Lanthanide Complexes – Austin Model 1) model where the lanthanide ion is simulated
by a sparkle implemented into the AM1 Hamiltonian used to perform a HF-SCF (Hartree-Fock Self-Consistent Field) calculation.
The previous implementation of the SMLC/AM1 model (sparkle/1) involving only two parameters has been generalized to be consistent
with the AM1 Hamiltonian and the new model (sparkle/2) significantly improved the prediction of molecular structures of Eu(III)
complexes. For the electronic spectra and energy level calculations of the lanthanide complexes the model replaces the metal
ion by a point charge with the ligands held in their positions as determined by the SMLC/AM1 model, and uses a INDO/S-CI (intermediate
neglect of differential overlap/spectroscopic-configuration interaction) model. A preliminary study of the solvent effects
on the absorption spectra of the free ligand is also presented. For the ligand-lanthanide ion energy transfer Fermi's golden
rule is used with the multipolar and exchange mechanisms being implemented and tested for several complexes. These theoretical
techniques have been applied to several complexes yielding very good results when compared to experimental data as well as
predictions for the molecular and electronic structures and the relative contributions of the mechanisms for the energy transfer
rates.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献
68.
Mutations in the X-linked E1 alpha subunit of pyruvate dehydrogenase: exon skipping, insertion of duplicate sequence, and missense mutations leading to the deficiency of the pyruvate dehydrogenase complex. 总被引:4,自引:2,他引:2 下载免费PDF全文
K Chun N MacKay R Petrova-Benedict A Federico A Fois D E Cole E Robertson B H Robinson 《American journal of human genetics》1995,56(3):558-569
Human pyruvate dehydrogenase (PDH)-complex deficiency is an inborn error of metabolism that is extremely heterogeneous in its presentation and clinical course. In a study of 14 patients (7 females and 7 males), we have found a mutation in the coding region of the E1 alpha gene in all 14 patients. Two female patients had the same 7-bp deletion at nt 927; another female patient had a 3-bp deletion at nt 931. Another female patient was found to have a deletion of exon 6 in her cDNA. Two other female patients were found to have insertions, one of 13 bp at nt 981 and one of 46 bp at nucleotide 1078. Two male patients were found to have a 4-bp insertion at nucleotide 1163. The remaining six patients all had missense mutations. A male patient and a female patient both had an A1133G mutation. The other missense mutations were C214T, C615A, and C787G (two patients). Five of these mutations are novel mutations, five have been previously reported in other patients, and two were published observations in other patients in an E1 alpha-mutation summary. In the four cases where parent DNA was available, only one mother was found to be a carrier of the same mutation as her child. 相似文献
69.
阳离子诱导大叶藻叶绿体膜中激发能在PSⅡ和PSⅠ之间分配变化的机理(英文) 总被引:1,自引:0,他引:1
用Ca2+ 和胰酶处理大叶藻(Zostera m arina)叶绿体膜研究了其类囊体膜多肽成分与Mg2+ 诱导其Chla荧光和类囊体膜表面电荷变化之间的相互关系,观察到:1.在正常的叶绿体膜中,Mg2+ 诱导PSⅡ荧光强度的增高与其诱导类囊体膜表面电荷密度的降低密切相关;2.用Ca2+ 处理这种叶绿体膜,除去类囊体膜表面的32~34 kD多肽对Mg2+ 诱导的上述现象无影响;3.如果用胰酶消化Ca2+ 处理过的叶绿体膜,进一步除去膜表面的26 kD多肽,Mg2+诱导的这些现象则全部消失。这些实验结果清楚地表明,在大叶藻的叶绿体膜中,类囊体膜表面的26 kD 多肽是阳离子诱导这两种相关现象的特异性作用部位。对阳离子调节激发能在PSⅡ和PSⅠ之间分配的机理进行了讨论 相似文献
70.
Thirty-four strains belonging to various species of the genus Thermus (T. aquaticus, "T. thermophilus,"
"T. brockianus,"
T. scotoductus, and genomic species 2) isolated from hot springs of different geographical areas were examined for plasmid content and restriction
fragment length polymorphism (RFLP) of plasmid DNAs. The four strains of the numerical taxonomy cluster E of genomic species
2 did not harbor plasmid DNA. Overall examination of the HindIII-RFLP profiling of plasmid DNA showed considerable variability between and within genomic species, with the exception
of presumed clonal isolates. In spite of this heterogeneity, HindIII plasmid digests within a numerical taxonomic cluster gave a subset of restriction fragments of similar or identical length.
Strains belonging to genomic species 2 or unclassified isolates from S. Pedro do Sul that harbored plasmid DNA (7 of the 14
strains studied) exhibited strong DNA homology between plasmid regions. No homologous sequences to these plasmid regions were
found in chromosomal DNA from strains isolated from S. Pedro do Sul in which no plasmids were detected. The strains belonging
to T. scotoductus formed two plasmid DNA homology groups, as estimated by probing with a plasmid fragment that coincided with the two numerical
taxonomy clusters proposed previously. Among the other species, homology of plasmid regions was also found between some strains.
Strong homology was also found between plasmid regions from some strains of different taxonomic groups, isolated from the
same and from different sources, suggesting that these sequences are highly conserved in plasmids present in Thermus. For plasmid-containing strains, results of plasmid RFLP profiling/DNA homology appear promising for the typing of Thermus at the level of biotypes or of individual strains, namely, for monitoring the diversity and frequency of isolates from a
particular hot spring.
Received: 24 October 1994 / Accepted: 6 March 1995 相似文献