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31.
JOSE L. HORREO JOSE L. MARTINEZ FERNANDO AYLLON IVAN G. POLA JOSE A. MONTEOLIVA MICHEL HÉLAND EVA GARCIA‐VAZQUEZ 《Freshwater Biology》2011,56(12):2567-2579
1. The effect of habitat fragmentation on freshwater species has been addressed using brown trout Salmo trutta L. as a model species with a dendritic population structure. 2. Microsatellite loci were employed as molecular markers. Levels of gene flow and population subdivision were determined in more than 1200 brown trout individuals inhabiting four south European rivers with contrasting patterns of fragmentation, defined by the presence of barriers. 3. The genetic units in the four rivers were restricted by artificial barriers, and gene flow among samples within each river was associated with the level of fragmentation of the river. 4. Loss of genetic diversity and dislocation of the dendritic model have been detected in fragmented rivers. These results emphasise the importance of mitigating the impact of dams by constructing passages to restore gene flow along the river, for fish and other migratory species, as well as the need for caution in relation to stocking in isolated areas to avoid problems of inbreeding. 相似文献
32.
RAQUEL XAVIER SALIHA ZENBOUDJI FERNANDO P. LIMA D. JAMES HARRIS ANTÓNIO M. SANTOS MADALENA BRANCO 《Biological journal of the Linnean Society. Linnean Society of London》2011,104(2):419-431
The transition zone between the Mediterranean and Atlantic basins has been extensively addressed in phylogeographical studies of marine species. However, biases exist towards the analysis of highly dispersive species, and there is a higher sampling effort in European coasts compared to North Africa. This may be hindering a detailed understanding of the historical and contemporary processes that shaped patterns of population genetic structure in the region. In the present study, we investigated the phylogeographical and phylogenetic patterns of mitochondrial cytochrome c oxidase subunit I sequences from a species with direct development and low dispersal abilities, Stenosoma nadejda (Rezig, 1989). The study area included 13 localities along the Atlantic and Mediterranean North African coasts, as well as the Alboran Sea. A new Stenosoma species, from the coasts of Algeria and Alboran Island, was discovered. For S. nadejda, phylogeographical analyses revealed three distinct clades: one in the Iberian Atlantic plus the Alboran Sea, one in the western Mediterranean, and another in the Atlantic coast of Africa. Haplotypes from the Alboran Island were more related to those from the western Mediterranean coast (east of the Almeria–Oran Front). Given the strong differentiation, it is probable that this species survived in multiple glacial refugia during the Pleistocenic glaciations. © 2011 The Linnean Society of London, Biological Journal of the Linnean Society, 2011, 104 , 419–431. 相似文献
33.
NEVES MARIA C. P.; DIDONET AGOSTINHO D.; DUQUE FERNANDO F.; DO?BEREINER JOHANNA 《Journal of experimental botany》1985,36(8):1179-1192
Neves, M. C. P, Didonet, A. D., Duque, F. F. and Dobereiner,J. 1985. Rhizobium strain effects on nitrogen transport anddistribution in soybeans.J. exp. Bot. 36: 11791192. The role of six Rhizobium strains in the nitrogen metabolismof soybeans (Glycine max Merril) was studied under glasshouseand field conditions. The strains could be divided into twogroups, group I which produced a large nodule mass with relativelylow efficiency and group II which produced less nodule massbut which fixed the same amount of nitrogen. Plants inoculatedwith group I strains remobilized nitrogen faster from leavesbut also lost more nitrogen in senesced leaves. Although thetotal nitrogen transported in the xylem was similar for allstrains, plants inoculated with group I strains contained lessnitrogen in ureides in the xylem sap during the whole growthcycle. This difference was reflected in the nitrogen partitioningwithin the shoot, and smaller nitrogen harvest indexes wereobserved in these plants than in those inoculated with strainsof group II. The role of ureides in the nitrogen partitioningand grain yield was confirmed by the significant correlationbetween mean ureide content in xylem sap and nitrogen partitioningor yield. Further, nodules formed with group I strains evolvedmore hydrogen than those formed with group II strains and thepossible significance of this is discussed. Key words: Ureides, hydrogen evolution, grain yield, grain yield, harvest index, nitrogen fixation 相似文献
34.
Gram-Chromotrope: a New Technique that Enhances Detection of Microsporidial Spores in Clinical Samples 总被引:3,自引:0,他引:3
HERCULES MOURA JORGE L. NUNES DA SILVA FERNANDO C. SODRÉ PATRICIA BRASIL KRISTY WALLMO SUSANNE WAHLQUIST SARA WALLACE GIAN P. CROPPO GOVINDA S. VISVESVARA 《The Journal of eukaryotic microbiology》1996,43(5):94S-95S
We have developed a new staining procedure that combines the traditional Gram staining for bacteria and the Weber's chromotrope staining method, the standard technique for the detection of microsporidia spores in clinical Specimens. This “Gram-chromotrope” staining technique enhances the staining characteristics of microsporidia spores and facilitates the easy detection and differentiation of spores from other microorganisms that are found in clinical specimens, especially stool samples. This new technique is fast, reliable, and simple to perform, and can be easily adapted for use in clinical laboratories. 相似文献
35.
FERNANDO ALFEREZ SHILA SINGH ANN L. UMBACH BRANDON HOCKEMA & JACQUELINE K. BURNS 《Plant, cell & environment》2005,28(11):1436-1449
The compound 5-chloro-3-methyl-4-nitro-1H-pyrazole (CMNP) is a pyrazole-derivative that induces abscission selectively in mature citrus (Citrus sinensis) fruit when applied to the canopy and has herbicidal activity on plants when applied to roots. Despite the favourable efficacy of this compound, the mode of action remains unknown. To gain information about the mode of action of CMNP, the effect of application to mature citrus fruit and Arabidopsis thaliana roots was explored. Peel contact was essential for mature fruit abscission in citrus, whereas root drenching was essential for symptom development and plant decline in Arabidopsis. CMNP was identified as an uncoupler in isolated soybean (Glycine max) mitochondria and pea (Pisum sativum) chloroplasts and an inhibitor of alcohol dehydrogenase in citrus peel, but not an inhibitor of protoporphyrinogen IX oxidase. CMNP treatment reduced ATP content in citrus peel and Arabidopsis leaves. Phospholipase A2 (PLA2) and lipoxygenase (LOX) activities, and lipid hydroperoxide (LPO) levels increased in flavedo of citrus fruit peel and leaves of Arabidopsis plants treated with CMNP. An inhibitor of PLA2 activity, aristolochic acid (AT), reduced CMNP-induced increases in PLA2 and LOX activities and LPO levels in citrus flavedo and Arabidopsis leaves and greatly reduced abscission in citrus and delayed symptoms of plant decline in Arabidopsis. However, AT treatment failed to halt the reduction in ATP content. Reduction in ATP content preceded the increase in PLA2 and LOX activities, LPO content and the biological response. The results indicate a link between lipid signalling, abscission in citrus and herbicidal damage in Arabidopsis. 相似文献
36.
ANGELA HAMPSHIRE C. S. LOPES FERNANDO COSTA E SILVA FILHO CELUTA S. ALVIANO ARON JURKIEWICZ JAYME ANGLUSTER WANDERLEY DE SOUZA 《The Journal of eukaryotic microbiology》1989,36(3):231-237
The effects of propranolol (10−3 mM) on the surface anionic groups of Herpetomonas muscarum muscarum were analysed by cell electrophoresis, by ultrastructural cytochemistry and by identification of sialic acids using paper chromatography. Differentiation of H. muscarum muscarum induced by propranolol treatment caused a significant increase in the net negative surface charge. Binding of cationized ferritin (CF) and colloidal iron hydroxide particles was observed at the cell surface of both untreated and propranolol-treated cells. In cells incubated in the presence of the drug the CF particles were distributed in all membrane regions. However, there were small areas where the particles were absent. In H. muscarum muscarum exposed to propranolol the density of residues of sialic acid per cell was higher, and the agglutinating activity with Sendai virus was more intense. However, the pattern of sialic acid, characterized by the presence of N-acetylneuraminic acid derivative, was not modified upon cell interaction with the drug. Treatment of both control and propranolol-treated protozoa with neuraminidase significantly reduced the surface charge. These findings suggest that sialic acid residues are the major anionogenic groups exposed on the surface of H. muscarum muscarum . 相似文献
37.
SYNOPSIS. Relationships between the cell cycle and the beginning of conjugation were analyzed for 3 hypotrichs: Diophrys scutum, Oxytricha bifaria, and Euplotes crassus. The first 2 species enter conjugation with micronuclei in G1; the latter species with a micronucleus in G2. The 1st micronuclear division of conjugating E. crassus is mitotic. Thus meiotic DNA replication occurs when the cells of each species have already entered the mating process. Cells from asynchronous populations start conjugation with their macronuclei primarily in G1 or more rarely at the beginning of the S stage in a percentage significantly different from that expected on the basis of random mating among all cells in the population. Also, macronuclear replication, when already begun, was blocked in cells undergoing conjugation. Therefore only the G1 or the very early S stages of the cell cycle are compatible with conjugation in the 3 analyzed species. 相似文献
38.
FERNANDO COSTA E SILVA FILHO WANDERLEY DE SOUZA 《The Journal of eukaryotic microbiology》1986,33(1):6-10
ABSTRACT. Drugs that interact with microtubules (colchicine and vinblastine) and microfilaments (cytochalasin B) partially inhibited cell growth and motility of Tritrichomonas foetus. Parasites incubated with these substances became rounded and cell division was blocked. Neither colchicine nor vinblastine disrupted the microtubules that form the peltar-axostylar system. Any one of these drugs interfered with the net negative surface charge of T. foetus as evaluated by determination of the cellular electrophoretic mobility (EPM). The decrease in the EPM of cytochalasin B-treated cells was caused by dimethylsulfoxide, which was used as solvent. Untreated cells as well as cytochalasin B-treated cells showed a uniform distribution of anionic sites on the plasma membrane as seen with cationized ferritin particles. In cells treated with colchicine or vinblastine the anionic sites were distributed in patches. These results are discussed in terms of participation of labile cytoplasmic microtubules and microfilaments in the control of the distribution of anionic sitecontaining macromolecules located on the cell surface of T. foetus. 相似文献
39.
FERNANDO VILLALTA H. STUART PANKRATZ FELIPE KIERSZENBAUM 《The Journal of eukaryotic microbiology》1987,34(3):285-290
Granules released from human eosinophils upon interaction with Trypanosoma cruzi amastigotes in vitro were seen attached to the surface of non-internalized parasites by electron microscopy. Amastigote damage was preceded by the binding of eosinophil granule material to its membrane, and eosinophil granule major basic protein (MBP) bound to the parasite surface was readily detectable. Additional evidence of eosinophil cytotoxicity for extracellular amastigotes was the observation that amastigotes trapped between two eosinophils, without being ingested by either one, were destroyed at the interface. Amastigotes isolated from the spleens of infected mice or grown in culture were similarly sensitive to the lytic effects of purified MBP. These results demonstrate the ability of human eosinophils to lyse T. cruzi amastigotes extracellularly in the absence of antibody and suggest that MBP may be involved in the effect. Thus, eosinophils, known to be capable of destroying phagocytosed amastigotes, could also contribute to the clearance of these parasites through extracellular killing. 相似文献
40.
ISABEL R. V. TEIXEIRA ANGEL R. BARCHUK LUANDA MEDEIROS FERNANDO S. ZUCOLOTO 《Physiological Entomology》2009,34(3):246-250
Abstract. In some insects, the finding of oviposition substrate triggers the uptake into oocytes of yolk proteins that are stored in the fat body during post‐embryonic development. The main host of the bean weevil Zabrotes subfasciatus (Coleoptera; Chrysomelidae; Bruchinae; Amblycerini), in which larval resources are the sole source for future egg maturation, is Phaseolus vulgaris. Despite not feeding as adults, females of this species are able to lay eggs after encountering host seeds but it is not known how females react to changes in the availability of bean seeds. In the present study, the behaviour of Z. subfasciatus facing two very different environments for oviposition is investigated, as well as how this influences offspring fitness. The results obtained show that females of Z. subfasciatus react to variations in the availability of seeds belonging to the same host species by adjusting egg size and number. Females on low bean seed density lay larger and fewer eggs than those on high bean seed density, demonstrating a trade‐off between these reproductive traits. Moreover, females can adjust egg size to changing levels of host availability during the first 4 days of their oviposition period. Although no difference in offspring weight is found, those from small eggs (low competition environment) result in larger adults. No response to selection on these traits after rearing beetles on the same host for 40 generations is observed. This unresponsiveness may indicate that beetle populations behave according to their reaction norm that already allows rapid adaptation to a varying amount of host‐seed availability and better exploitation of the environments of this widespread stored‐seed pest. 相似文献