The Interatheriinae from the Late Oligocene of Mendoza (Argentina), with comments on some Deseadan Interatheriidae

This contribution discusses the interatheriids from Quebrada Fiera, a Late Oligocene locality in the south of Mendoza Province. In addition, the taxonomic status of the Deseadan interatheres Plagiarthrus clivus, Argyrohyrax proavus, Argyrohyrax proavunculus, ?Argyrohyrax acuticostatus, Progaleopithecus fissurelatus, Progaleopithecus tournoueri, and Notohyrax conicus is discussed. We recognise in Quebrada Fiera A. proavus and Progaleopithecus sp. A possible third taxon is represented by one specimen that shows a combination of features that do not permit accurate assignment. These new remains, in combination with a systematic study of Deseadan interatheres, allow for a better definition of each recognised taxon, extended diagnoses, and clarification of some long-standing taxonomic misconceptions. Indeed, the priority of A. proavus over P. clivus is established, and the latter is proposed as a nomen dubium. Associated deciduous and permanent dentition and some limb bones provide new data on the ontogenetic variation and postcranial morphology of Argyrohyrax. Phylogenetic analysis supports the taxonomic determinations and places A. proavus in a basal position with respect to Archaeophylus or Progaleopithecus. Documented fossil records of Argyrohyrax and Progaleopithecus in Mendoza extend the known range to middle latitudes in central west Argentina; these taxa expand the list of mammals from Quebrada Fiera with Patagonian affinities.     

New Alicornops (Rhinocerotidae) remains from Lower and Middle Siwaliks,Pakistan

Unpublished rhinocerotid remains from the Lower and Middle Siwaliks of Pakistan are described in this paper and recognized as two species of Alicornops. Alicornops complanatum (Heissig) is identified in the Dhok Pathan Formation of the Middle Siwaliks and Alicornops laogouense Deng in the Kamlial Formation of the Lower Siwaliks. The Dhok Pathan Formation levels with A. complanatum are roughly correlated with the late Miocene-Pliocene European mammal zones MN10-15. In turn, levels with A. laogouense of the Kamlial Formation would correlate with the middle–late Aragonian (middle Miocene) European MN5. The recognition of the Chinese species A. laogouense in the Potwar Plateau represents the first discovery of this taxon in Pakistan and increases the geographical and stratigraphic distributions of this species, and adds to the rhinocerotid association from the Siwaliks. In turn, the presence of A. complanatum in the Siwaliks of Potwar Plateau also enlarges its geographic distribution in Pakistan, as it was previously known from the Bugti Hills of Balouchistan. The absence of Alicornops from the Siwaliks in the Chinji and Nagri formations (between late MN5 and MN9 zones) might be due to an inadequate fossil record, as other rhinocerotid species are known from Kamlial to Dhok Pathan formations. However, the two recorded species of Alicornops could also reveal two independent migration waves as supported by the appearance of other taxa in different formations. A summary of fossil Cenozoic rhinocerotids from different areas of Pakistan is also presented.     

Improved bioethanol production in an engineered Kluyveromyces lactis strain shifted from respiratory to fermentative metabolism by deletion of NDI1

In this paper, we report the metabolic engineering of the respiratory yeast Kluyveromyces lactis by construction and characterization of a null mutant (Δklndi1) in the single gene encoding a mitochondrial alternative internal dehydrogenase. Isolated mitochondria of the Δklndi1 mutant show unaffected rate of oxidation of exogenous NADH, but no oxidation of matrix NADH; this confirms that KlNdi1p is the only internal NADH dehydrogenase in K. lactis mitochondria. Permeabilized cells of the Δklndi1 mutant do not show oxidation of matrix NADH, which suggests that shuttle systems to transfer the NADH from mitochondrial matrix to cytosol, for being oxidized by external dehydrogenases, are not functional. The Δklndi1 mutation decreases the chronological life span in absence of nutrients. The expression of KlNDI1 is increased by glutathione reductase depletion. The Δklndi1 mutation shifts the K. lactis metabolism from respiratory to fermentative: the Δklndi1 strain shows reduced respiration rate and increased ethanol production from glucose, while it does not grow in non-fermentable carbon sources such as lactate. The biotechnological benefit of the Δklndi1 mutant for bioethanol production from waste cheese whey lactose was proved.