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331.
Adam H. Balen Jovita Er Brian Rafferty Matthew Rose 《In vitro cellular & developmental biology. Animal》1995,31(4):316-322
Summary We have described the protocols and characterization of a pituicyte culture, which became established as a reliable and reproducible
bioassay for the secretion of follicle-stimulating hormone (FSH) and luteinizing hormone (LH). The bioassay was used to measure
the bioactivity of factors that inhibit and stimulate gonadotrophin secretion. The protocol that was used involved the culling
of female Wistar rats (200 to 250 g weight), at random stages of their cycle, and dispersal of their pituicytes in a concentration
of 0.4 × 106 cells · ml−1 · well−1 in serum-free medium (Dulbecco’s modified Eagle’s medium/Ham’s F12 mixture, supplemented with insulin and transferrin) in
Falcon 3047 24-well culture plates. After 24 h of pre-culture, the medium was changed and the cells cultured for a further
48 h. The supernatant was removed and assayed for basal secretion of FSH and LH. The cells were then stimulated with 10−8
M GnRH for 4 h and the supernatant assayed for gonadotrophin-releasing hormone (GnRH)-stimulated FSH and LH secretion. All
samples were assayed as pairs of duplicates (i.e. quadruplicate samples) which were randomly added to the plates to minimize
plate effects. Random number tables were used to achieve this randomization. 相似文献
332.
Immunohistochemical staining of fibronectin is proposed as a good method for demonstrating capillaries in skeletal muscle tissue. The reaction superimposed on the histochemical reaction for myofibrillar ATPase enables simultaneous demonstration of fibre typing and capillary supply on the same tissue section. The method is quick and makes possible automated image analysis. 相似文献
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334.
Abstract
Context
Acrylamide (ACR) is now a risk for general public health. Argan oil (AO) is harvested from the fruits of Argania spinosa and its rich source of antioxidant and phenolic compounds. 相似文献335.
Nayely Garibay-Nieto Brianda Areli Hernández-Morán Eréndira Villanueva-Ortega María José Garcés-Hernández Karen Pedraza-Escudero América Arroyo-Valerio César Pedraza-Helvert Arturo Herrera-Rosas Estibalitz Laresgoiti-Servitje Mireya León-Hernández Neyla Baltazar-López Joselin Hernández-Ruíz Fabiola Serratos-Canales Gloria Queipo-García Sarah Elizabeth Messiah Srinivas Mummidi Juan Carlos López-Alvarenga 《Endocrine practice》2022,28(3):315-320
ObjectiveTo describe a third-degree polynomial function (hysteresis) of the effect size of age, obesity, and insulin sensitivity over the carotid intima-media thickness (c-IMT), in the pediatric and adult groups.MethodsA quasi-experimental study with fixed factor analysis of age (children aged 8-12 years, n = 73; adults aged 21-45 years, n = 82) and obesity (yes, n = 76; no, n = 79) was conducted to analyze the effect on the c-IMT and Matsuda insulin sensitivity index values. This quasi-experimental design was analyzed with robust regression modeling.ResultsThe additive effect of obesity, independent of age, was evident in the case of the c-IMT values. There was no interaction effect, but a significant difference between participants with normal weight and those with obesity was found (P < .0001). The difference between adults and children was also significant, but the effect size was smaller. A model was created based on age, Tanner stage, and obesity using the c-IMT and Matsuda insulin sensitivity index values. A linear function fit as R2, and the cubic function estimated parameters like a polynomial model.ConclusionThis practical study design showed that children with obesity displayed the same levels of carotid intima-media abnormalities as adults with obesity. The polynomial shape of the model suggests potentially poor outcomes that resemble the hysteresis process and may predict chronic cardiometabolic events during early adulthood. 相似文献
336.
Pre- and postnatal superior cervical ganglia of the rat were cultured in Rose chambers for 1-7 days with or without hydrocortisone. Phenylethanolamine-N-methyltransferase (PNMT) was demonstrated by indirect immunofluorescence technique. In cultures without added hydrocortisone, no cells or fibres showed PNMT-immunoreactivity, without regard to the time in culture or the developmental stage at the time of explantation. The first PNMT-immunoreactive cells in hydrocortisone-containing cultures appeared 3 days after the explantation of E14 ganglia, or 1 day after the explantation of E15 ganglia, i.e. at the developmental stage E16-E17. The cultures of neither E14 nor E15 ganglia showed marked fibre growth from the PNMT-immunoreactive cell bodies. On the other hand, in the hydrocortisone-containing cultures of newborn or postnatal rats, there was extensive nerve fibre formation from the PNMT-immunoreactive cells in the course of the culture. PNMT-immunoreactive cells did not appear in hydrocortisone-containing cultures of ganglia taken from rats older than 17 postnatal days. 相似文献
337.
Hasan Tanak Ferda Erşahin Yavuz Köysal Erbil Ağar Şamil Işık Metin Yavuz 《Journal of molecular modeling》2009,15(10):1281-1290
The Schiff base compound, N-n-Decyl-2-oxo-5-nitro-1-benzylidene-methylamine, has been -synthesized and characterized by IR,
electronic spectroscopy, and X-ray single-crystal determination. Molecular geometry from X-ray experiment of the title compound
in the ground state have been compared using the Hartree-Fock (HF) and density functional method (B3LYP) with 6-31G(d) basis
set. Calculated results show that density functional theory (DFT) at B3LYP/6-31G(d) level can well reproduce the structure
of the title compound. To investigate the solvent effect for the atomic charge distributions of the title compound, self-consistent
reaction field theory with Onsager reaction field model was used. In addition, DFT calculations of the title compound, molecular
electrostatic potential and thermodynamic properties were performed at B3LYP/6-31G(d) level of theory. 相似文献
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