Die Systematik der Actinomyceten

Summary Before the modern definition of the genereActinomyces, Micromonospora andProacgtinomyces, a historical-critical review of Actinomycetes (ray-fungi), based on personal, present and past, researches, has been made. A modern, analytical key for all Families, Subfamilies and Genera of Actinomycetales Buchanan, has been furnished. Of particular-importance is the discussion on the reproductive bodies and on the general morphology, fundamental for the taxonomy of Actinomycetes.A few selected, most important species are accurately described (with sinonimy) and discussed from the historic-experimental points of view, namely:Actinomyces Bostroemi, A. albus, A. sulpureus, A. carneus, A. Madurae, A. viridis, A. melanosporeus, A. innominatus andProactinomyces asteroides.

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Sumario El Autor, después de una sintesis histórico-critica sobre los Actinomicetos, en base a sus proprias buscas experimentales actuales y passadas, formula la moderne diagnosis de los génerosActinomyces, Micromonospora, Proactinomuces. etc., integrando, además, su estudio con una llave analitica moderna de todas las Familias, Subfamilias y Generos de los Actinomycetales Buchanan. De particular impprtancia es la discusión sobre la naturaleza de los cuerpos reproductivos de los Actinomicetos, y, màs generalmente, de la morfologia que está a la base de su sistematica.El Autor incluye la descripción cuidadosa de unas principales especies, reexaminadas desde el punto de vista historico-experimental, con sus ocmpleta sinonimia. Estas especies sonActinomyces Bostroemi, A. albus, A. sulphureus, A. carneus, A. Madurae,A. viridis, A. melanosporeus, A. innominatus, yProactinomyces asteroides.

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Riassunto L'autore, dopo una sintesi storico-critica sugli Actinomiceti, sulla base di proprie ricerche sperimentali recenti e passate, formula la diagnosi moderna dei generiActinomyces, Micromonospora, Proactinomyces, ecc. fornendo inoltre una moderna chiave sistematica per tutte le Famiglie, Sottofamiglie, e Generi degli Actinomycetales Buchanan. Di particolare importanza è la discussione sulla natura dei corpi riproduttivi degli Actinomiceti ed in genere sulla morfologia che sta a base della sistematica.Include la descrizione accurata di alcune delle principali specie, revisionate dal punto di vista storico-critico e sperimentale; queste specie sono provviste della completa sinonimia (Actinomyces Bostroemi, A. albus, A. sulpkureus, A. carneus, A. Madurae, A. viridis, A. melanosporeus, A. innominatus, Proactinomyces asieroides).

     

Behaviour of several 'progression markers' during the HIV-Ab seroconversion period. Comparison with later stages.

Two acute phase reactants, four cytokines, five soluble factors and lymphocyte subpopulations have been simultaneously evaluated in 16 subjects before and closely after the HIV-Ab seroconversion time. The same variables have also been determined in 50 HIV-Ab-negative high risk subjects, in 36 CDC II-III and in 30 CDC IV patients, utilizing a mixed longitudinal epidemiological model. The results show significant variations of few parameters in the early phases (increase: sCD8, beta-2-Microglobulin, sIL-2R, sCD23, Neopterin, IFN-alpha; decrease: CD4+ lymphocytes). In the course of the disease, many others parameters progressively increase (IFN-tau, IL-4, IL-6, acid-alpha 1-glycoprotein, alpha 1-antitrypsin) or decrease (B- and T-lymphocytes). Ferritin, in particular, highly increases only in CDC IV stage. These data may be useful to monitor patients during the entire course of their disease and to suggest the time elapsed from seroconversion.     

Cell cycle kinetic analysis with flow cytometry in pea root meristem synchronized with aphidicolin

Meristematic cells in pea root were synchronized after treatment with aphidicolin. Flow cytometric analysis of DNA content variations revealed a variety of information: ca 50% of the cells were synchronized in the first part of the S phase; kinetic studies of the S-phase traverse revealed an increase of the DNA synthesis rate from early to late S; some of the cells in the meristem were non-cycling, arrested in Gl (19%) and G2 (12%). The possibility of using such a synchronized system in cytogenetic and biomolecular studies is discussed.