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111.
112.
Carolina V. Messias Eliane Santana-Van-Vliet Julia P. Lemos Otacilio C. Moreira Vinicius Cotta-de-Almeida Wilson Savino Daniella Arêas Mendes-da-Cruz 《PloS one》2016,11(1)
Sphingosine-1-phosphate (S1P) is a bioactive sphingolipid involved in several physiological processes including cell migration and differentiation. S1P signaling is mediated through five G protein-coupled receptors (S1P1-S1P5). S1P1 is crucial to the exit of T-lymphocytes from the thymus and peripheral lymphoid organs through a gradient of S1P. We have previously observed that T-ALL and T-LBL blasts express S1P1. Herein we analyzed the role of S1P receptors in the migratory pattern of human T-cell neoplastic blasts. S1P-triggered cell migration was directly related to S1P1 expression. T-ALL blasts expressing low levels of S1P1 mRNA (HPB-ALL) did not migrate toward S1P, whereas those expressing higher levels of S1P1 (MOLT-4, JURKAT and CEM) did migrate. The S1P ligand induced T-ALL cells chemotaxis in concentrations up to 500 nM and induced fugetaxis in higher concentrations (1000–10000 nM) through interactions with S1P1. When S1P1 was specifically blocked by the W146 compound, S1P-induced migration at lower concentrations was reduced, whereas higher concentrations induced cell migration. Furthermore, we observed that S1P/S1P1 interactions induced ERK and AKT phosphorylation, and modulation of Rac1 activity. Responding T-ALL blasts also expressed S1P3 mRNA but blockage of this receptor did not modify migratory responses. Our results indicate that S1P is involved in the migration of T-ALL/LBL blasts, which is dependent on S1P1 expression. Moreover, S1P concentrations in the given microenvironment might induce dose-dependent chemotaxis or fugetaxis of T-ALL blasts. 相似文献
113.
Stephanie Boudon Marc Heidl Anna Vuorinen Eliane Wandeler Remo Campiche Alex Odermatt Eileen Jackson 《Bioorganic & medicinal chemistry》2018,26(18):5128-5139
The enzyme 11β-HSD1 plays a crucial role in the tissue-specific regulation of cortisol levels and it has been associated with various diseases. Inhibition of 11β-HSD1 is an attractive intervention strategy and the discovery of novel selective 11β-HSD1 inhibitors is of high relevance. In this study, we identified and evaluated a new series of selective peptide 11β-HSD1 inhibitors with potential for skin care applications. This novel scaffold was designed with the aid of molecular modeling and two previously reported inhibitors. SAR optimization yielded highly active peptides (IC50 below 400?nM) that were inactive at 1?µM concentration against structurally related enzymes (11β-HSD2, 17β-HSD1 and 17β-HSD2). The best performing peptides inhibited the conversion of cortisone into cortisol in primary human keratinocytes and the most active compound, 5d, was further shown to reverse cortisone-induced collagen damage in human ex-vivo tissue. 相似文献
114.
Tina L. Yuan Arnaud Amzallag Rachel Bagni Ming Yi Shervin Afghani William Burgan Nicole Fer Leslie A. Strathern Katie Powell Brian Smith Andrew M. Waters David Drubin Ty Thomson Rosy Liao Patricia Greninger Giovanna T. Stein Ellen Murchie Eliane Cortez Frank McCormick 《Cell reports》2018,22(7):1889-1902
115.
Eliane de O. Borges Camila B. C. Martins Rodolfo R. da Silva Paulo H. G. Zarbin 《Arthropod-Plant Interactions》2018,12(1):123-131
In response to injury, plants produce volatile organic compounds (VOCs) that usually differ depending on the type of damage they have suffered (e.g., mechanical damage, herbivory, and oviposition). The objectives of this study were to identify and compare the bouquet of volatiles emitted by passion vine plants (Passiflora edulis) after injury caused by mechanical damage (MD), herbivory (HB), and oviposition (OV) by the lepidopteran, Heliconius erato phyllis. Following injury, extracts of plant emissions were collected from each treatment every 24 h for three days and were analyzed by GC and GC/MS. Results show that plants emitted 12 volatiles before and after damage, namely terpenoids, ketones, and aldehydes. Although no significant differences were detected between the three treatments individually, if the entire bouquet of volatiles is analyzed, samples collected at 24 h were different from samples collected at 48 and 72 h. However, terpenoid emission increased significantly in HB plants after 24 h. HB plants emitted approximately 6300, 50, 46, 11, 6, and 3.6 times more (3E,7E)-4,8,12-trimethyl-1,3,7,11-tridecatetraene, (E)-4,8-dimethyl-1,3,7-nonatriene (DMNT), (E)-β-ocimene, (Z)-β-farnesene, (E)-β-caryophyllene, and farnesane, respectively, compared to control plants. OV plants displayed a peak of emission of (E)-β-ocimene after 72 h, which distinguished them from HB plants. MD plants showed a general increase of VOCs versus undamaged control plants. Furthermore, it has been suggested that (E)-β-ocimene may be sequestered by larvae of H. erato phyllis as a component of the odoriferous bouquet of the abdominal scent glands present in adult males, which play a role in sexual communication. 相似文献
116.
Catherine Garitte Jean-Pierre Almodovar Eliane Benjamin Céline Canhao 《Twin research》2002,5(6):538-543
The purpose of this study was to examine the impact of the sex makeup of pairs of twins on language acquisition. Past research indicated that this variable plays a role in speech problems of twin children. The questions raised were whether being a boy or a girl and having a boy or girl co-twin affected linguistic performance. A language test was given to 30 pairs of boy-girl twins, 16 pairs of boy twins, and 16 pairs of girl twins whose average age was 4 years 8 months. Their test scores confirmed our hypotheses. The poorest performance was obtained by the boy twin pairs and the best performance, by either the girl twin pairs or the different-sex pairs. The results were interpreted in the light of findings on language learning differences between girls and boys, and also in terms of Vygotsky's zone of proximal development. 相似文献
117.
Eliane Didier 《Development genes and evolution》1973,172(4):287-302
Résumé L'analyse de certains points de la morphogénèse du canal de Müller et du rôle joué par le canal de Wolff dans la formation de l'oviducte a été abordée à l'aide de diverses méthodes expérimentales (blocage de l'ébauche müllérienne par un obstacle mécanique ou par une irradiation localisée par les rayons X).Les résultats obtenus soulignent la formation et l'évolution indépendante des 2 tissus, l'epithélium interne et le mésenchyme périphérique, qui composent la jeune ébauche müllérienne.L'épithélium interne se met en place par le jeu de la croissance crânio-caudale d'une ébauche primitive formée au niveau de l'ostium et induite par le canal de Wolff.Le mésenchyme périphérique se forme «in situ», aux dépens de la crÊte mullérienne qui est elle-mÊme induite par le canal de Wolff.Chez les Oiseaux, le développement de l'oviducte reste donc dépendant de l'uretère primaire, du fait de la double action inductrice exercée par le canal de Wolff sur les deux tissus constituant primitivement le canal de Müller.
Researches on Mullerian duct morphogenesis in birdsII. Experimental study
Summary Certain points in the Mullerian duct morphogenesis, and the role played by the Wolffian duct in oviduct formation, were studied with some experimental methods (by stopping the mullerian anlage with a mechanical obstacle, or by a localized irradiation with X-rays).The results emphasize the independent formation and evolution of the two tissues, the inner epithelium and the mesenchyme which compose the young mullerian anlage.The inner epithelium is formed by the cranio-caudad outgrowth of a primitive anlage, formed at the level of the ostium, and induced by the Wolffian duct.The peripheric mesenchyme is formed in situ, from the mullerian strip, which itself is induced by the Wolffian duct.In Birds, oviduct development depends upon the primary ureter, by means of the double inductive action exerted by the Wolffian duct upon both tissues constituting the Mullerian duct anlage.相似文献
118.
Franois Coudor Nicolas Authier Delphine Guillaume Axel Bal Eliane Duroux Joseph Fialip 《Journal of chromatography. B, Analytical technologies in the biomedical and life sciences》1999,721(2):192
A simple and sensitive high-performance liquid chromatographic method is described for the determination of paclitaxel (Taxol®) at 230 nm using a Nucleosil C18 (5 μm) column and a methanol–water (70:30, v/v) mobile phase following a single-step extraction from serum with dichloromethane. The assay was validated against the classical criteria and was applied to a toxicokinetic study in rats after one or five, one per week) intraperitoneal administrations of 16 mg/kg Taxol®. 相似文献
119.
Felipe de Oliveira Fernandes Sérgio Ricardo de Oliveira Vitor Klein Marcella Araújo do Amaral Carneiro Pio Colepicolo Eliane Marinho-Soriano 《Journal of applied phycology》2017,29(2):695-705
The genus Gracilaria is one of the most important sources of agar in the world. In Brazil, Gracilaria birdiae is the main commercially exploited species; however, overexploitation has contributed to the depletion of natural beds. In order to obtain more information so as to consolidate G. birdiae cultivation, studies under laboratory (indoor and outdoor) and field (sea and shrimp pond) conditions were conducted to evaluate the effects of fertilizer pulses on biomass and relative growth rate (RGR) of this species. The following nutrient sources used were (T1) shrimp-pond effluent, (T2) fertilizer for aquarium plants (Mbreda), and (T3) fertilizer extract of Ascophyllum nodosum (Acadian). Significant differences for growth were recorded over time for all treatments in both outdoor and field conditions (p < 0.001). The highest RGRs were recorded for treatments that used pulses of commercial fertilizers (T2 and T3) and the lowest for treatment using shrimp-pond effluent pulses (T1). The analysis of the nutrient content in tissue also showed a relationship between growth and nitrogen and phosphorus accumulation in the algal tissues. The N/P ratio indicated a significant effect on the growth of G. birdiae and the highest RGRs were registered for seedlings with a N/P ratio ≥16 (T2 and T3). In conclusion, the best results were recorded for the Mbreda and Acadian commercial fertilizers. However, although no significant differences were detected between growth and the two fertilizers (T2 and T3), the seedlings cultivated under Acadian pulses showed a better performance against environmental stress caused by reduced salinity. 相似文献
120.
Figueiredo Douglas B. Carvalho Eneas Santos Mauricio P. Kraschowetz Stefanie Zanardo Rafaela T. Campani Gilson Silva Gabriel G. Sargo Cíntia R. Horta Antonio Carlos L. de C. Giordano Roberto Miyaji Eliane N. Zangirolami Teresa C. Cabrera-Crespo Joaquin Gonçalves Viviane Maimoni 《Applied microbiology and biotechnology》2017,101(6):2305-2317
Applied Microbiology and Biotechnology - Streptococcus pneumoniae is the main cause of pneumonia, meningitis, and other conditions that kill thousands of children every year worldwide. The... 相似文献