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11.
Slow and spike potentials were recorded from single cells in the receptor layer of the compound eye of the drone of the honeybee. From electron microscopic observation of the drone ommatidium, it was concluded that the response had been recorded from the retinula cell. The following hypothesis is suggested for the initiation of spike potentials in the drone compound eye: Photic stimulation results in a decrease in the resistance of all or part of the retinula cell membrane, giving rise to the retinal action potential. The retinal action potential causes outflow of the current through the proximal process of the cell. This depolarizing current initiates spike potentials in the proximal process or axon of the retinula cell which are recorded across the soma membrane of the retinula cell. 相似文献
12.
The Development of the Rhabdom and the Appearance of the Electrical Response in the Insect Eye 总被引:4,自引:2,他引:2 下载免费PDF全文
Electron microscopic studies on the development of the rhabdom in the compound eye of the silkworm moth and pupa (Bombyx mori) were carried out in parallel with the recording of the electrical response to photic stimulation. No electrical response to photic stimulation was recorded from the pupal compound eye which had no trace of differentiation of the rhabdom. With the differentiation of development of the rhabdom in the pupal compound eye, electrical responses could be recorded, and the amplitude of such electrical responses increased with the progress of development of the rhabdom. These observations suggest that the rhabdom is probably the site of the photochemical reaction which leads to the generation of the slow retinal action potentials. 相似文献
13.
Summary Differential increases in the numbers of pinocytotic vesicles, multivesicular bodies and total complex bodies occurred in the cytoplasm of specific photoreceptor cells in the compound eye of the crab Libinia exposed for six hours to polarized light with various e-vector orientations. These data coupled with previous results on the same species proved that the seven retinular cells in each ommatidium formed two functional groups selectively light adaptable by e-vectors oriented 90° apart. One group (Channel I, comprising Cells 1, 4 and 5) was more affected by horizontal polarization; the other (Channel II, comprising Cells 2, 3, 6 and 7) was more affected by vertical polarization.This confirmed by a quite independent technique the conclusion reached from electrophysiological experiments on the crab Cardisoma that decapod compound eyes have two orthogonal polarization analyzer channels. In addition the present data showed that both channels occur in each ommatidium as hypothesized on previous electron microscopic evidence and that the axes of maximum absoprtion in the two retinal channels were parallel to the long axes of their cells' rhabdom microvilli, horizontal in Channel I and vertical in Channel II. The latter relations in turn supported the hypothesis that the dichroism of rhodopsin was fundamental to the analyzer mechanism.This research has been supported by U. S. Air Force Grant AFOSR 1064 and NASA Grant NGR 07-004-055. The authors wish to thank Professor Joseph G. Gall for generously sharing his electron microscope facilities. 相似文献
14.
Summary The cytological influence of light and dark adaptation (LA and DA) on the retinular cells of the spider crab Libinia emarginata has been studied by light and electron microscopy in four adaptive states: 17 hours darkness, 5 hours darkness, 5 hours diffuse light and 17 hours diffuse light. The rhabdom's fine structure is typical of decapods but its dual overall form and position mingle certain features of both apposition and superposition compound eye types. Distal and proximal retinal pigments both showed adaptive migration, but the distal pigment cells moved over a restricted range, and DA separated the retinular cell pigment granules into two groups, perinuclear and basilar.In the rhabdom no changes in its position, dimensions or microvillus fine structure were observed with LA or DA. But at the base of the rhabdom microvilli the rate of pinocytosis was strongly affected by the eye's adaptive state, being lowest after 17 hours DA and greatest after 17 hours LA; the wall of the 0.1 microvesicles so formed, looked like the membrane of the rhabdom microvillus and they were the same size as the vesicles in multivesicular bodies and in vesicular lamellar bodies.Three categories of complex cytoplasmic particles about 1 in diameter (multivesicular bodies, vesicular lamellar bodies and purely lamellar bodies) were all increased in number by decreased DA and by increased LA; similar quantitative effects occurred in the endoplasmic reticulum and in the ribosomes.The pinocytotic vesicles and the complex cytoplasmic bodies may represent part of an intracellular system to dispose of rhabdom metabolites whose production was initiated or increased by light absorption.Cytoplasmic and perirhabdomal vacuoles mainly distal in location, were also affected by light, but inversely; their maximal extent occurred after 17 hours DA; less DA or any LA significantly decreased their presence and aggregation.The data reported are of interest not only because they correlate retinal fine structure with the metabolism of vision but also because they provide a new and specific tool for distinguishing active from inactive neurosensory cells in the optic pathway.This research was initiated with the aid of U.S. Public Health Service Grant NB-03076 and has been continued with the support of U.S. Air Force Grant AFOSR-1064. The authors wish to thank Dr. Joseph G. Gall and Dr. William R. Adams for generously sharing their electron microscopic facilities; they are also grateful to Mrs. Mabelita Campbell for her collaboration on the light microscopy. 相似文献
15.
N-hydroxy-2-naphthylamine (NOH-2NA). an active metabolite of human occupational bladder carcinogens, induced, in V79 Chinese hamster cells. chromosomal aberrations which were suppressed in the presence of catalase and/or superoxide dismutase. The induction of the aberrations was more efficient in a more basic pH in parallel with the generation of hydrogen peroxide from NOH-2NA. The possible role of the oxidation product of NOH-2NA in the induction of the aberrations is discussed. 相似文献
16.
K Ochi 《Journal of general microbiology》1990,136(12):2405-2412
A relaxed (rel) mutant was found among 70 thiopeptin-resistant isolates of Streptomyces coelicolor A3(2) which arose spontaneously. The ability of the rel mutant to accumulate ppGpp during Casamino acid deprivation was reduced 10-fold compared to the wild-type. Analysis of the ribosomal proteins by two-dimensional PAGE revealed that the mutant lacked a ribosomal protein, tentatively designated ST-L11. It was therefore classified as a relC mutant. The mutant was defective in producing A-factor and the pigmented antibiotic prodigiosin, in both liquid and agar cultures, but produced agarase normally. Production of actinorhodin, another pigmented antibiotic, was also abnormal; it appeared suddenly in agar cultures after 10 d incubation. Although aerial mycelium still formed, its appearance was markedly delayed. Whereas liquid cultures of the parent strain accumulated ppGpp, agar cultures accumulated only trace amounts. Instead, a substance characterized only as an unidentified HPLC peak accumulated intracellularly in the late growth phase, just before aerial mycelium formation and antibiotic production. This substance did not accumulate in mutant cells. It was found in S. lividans 66 and S. parvulus, but not in seven other Streptomyces species tested. The significance of these observations, and the relationship of the mutant to earlier rel isolates of Streptomyces is discussed. 相似文献
17.
Hidetaka Kosako Yukiko Gotoh Eisuke Nishida 《Development, growth & differentiation》1996,38(6):577-582
Mitogen-activated protein kinase (MAPK) was originally identified as a serine/threonine protein kinase that is rapidly activated in response to various growth factors and tumor promoters in mammalian cultured cells. The kinase cascade including MAPK and its direct activator, MAPK kinase (MAPKK), is now believed to transmit various extracellular signals into their intracellular targets in eukaryotic cells. It has been reported that activation of MAPKK and MAPK occurs during the meiotic maturation of oocytes in several species, including Xenopus laevis . Studies with neutralizing antibodies against MAPKK, MAPK phosphatases and constitutively active MAPKK or MAPK have revealed a crucial role of the MAPKK/MAPK cascade in a number of developmental processes in Xenopus oocytes and embryos. 相似文献
18.
Induction of actinorhodin production by rpsL (encoding ribosomal protein S12) mutations that confer streptomycin resistance in Streptomyces lividans and Streptomyces coelicolor A3(2). 总被引:8,自引:0,他引:8 下载免费PDF全文
A strain of Streptomyces lividans, TK24, was found to produce a pigmented antibiotic, actinorhodin, although S. lividans normally does not produce this antibiotic. Genetic analyses revealed that a streptomycin-resistant mutation str-6 in strain TK24 is responsible for induction of antibiotic synthesis. DNA sequencing showed that str-6 is a point mutation in the rpsL gene encoding ribosomal protein S12, changing Lys-88 to Glu. Gene replacement experiments with the Lys88-->Glu str allele demonstrated unambiguously that the str mutation is alone responsible for the activation of actinorhodin production observed. In contrast, the strA1 mutation, a genetic marker frequently used for crosses, did not restore actinorhodin production and was found to result in an amino acid alteration of Lys-43 to Asn. Induction of actinorhodin production was also detected in strain TK21, which does not harbor the str-6 mutation, when cells were incubated with sufficient streptomycin or tetracycline to reduce the cell's growth rate, and 40 and 3% of streptomycin- or tetracycline-resistant mutants, respectively, derived from strain TK21 produced actinorhodin. Streptomycin-resistant mutations also blocked the inhibitory effects of relA and brgA mutations on antibiotic production, aerial mycelium formation or both. These str mutations changed Lys-88 to Glu or Arg and Arg-86 to His in ribosomal protein S12. The decrease in streptomycin production in relC mutants in Streptomyces griseus could also be abolished completely by introducing streptomycin-resistant mutations, although the impairment in antibiotic production due to bldA (in Streptomyces coelicolor) or afs mutations (in S. griseus) was not eliminated. These results indicate that the onset and extent of secondary metabolism in Streptomyces spp. is significantly controlled by the translational machinery. 相似文献
19.
Ras-Regulated Hypophosphorylation of the Retinoblastoma Protein Mediates Neuronal Differentiation in PC12 Cells 总被引:2,自引:0,他引:2
†HongZhen Li ‡Hiroshi Kawasaki ‡Eisuke Nishida Seisuke Hattori Shun Nakamura 《Journal of neurochemistry》1996,66(6):2287-2294
Abstract: To investigate the role of the retinoblastoma protein pRB in neuronal differentiation, we have measured the accumulation of hypophosphorylated pRB in PC12 cells stimulated by nerve growth factor (NGF). NGF induced the accumulation of hypophosphorylated pRB within 30 min and the level peaked after 12 h. Viral Kiras, cyclic AMP (cAMP), and 12- O -tetradecanoylphorbol 13-acetate (TPA) also induced the hypophosphorylation of pRB, but epidermal growth factor and interleukin-6 did not. The extent of hypophosphorylation of pRB correlated well with the capacity of these factors to stimulate neurite outgrowth. The constitutively activated Ras induced persistent shift of the phosphorylation state of pRB toward hypophosphorylation. A dominant negative form of cHa-Ras suppressed significantly induction of the hypophosphorylation of pRB by NGF, but not by cAMP. Taken together, these results suggest that the hypophosphorylation of pRB triggered by NGF is mediated by a Ras-dependent pathway. Furthermore, microinjection of a monoclonal antibody specific for the hypophosphorylated form of pRB blocked the neurite outgrowth initiated by NGF. These results suggest a crucial role of pRB in withdrawal of cells from the cell cycle and in neuronal differentiation of PC12 cells. 相似文献
20.
Synopsis We collected schools of young, guarded by parents, of six common cichlid species to investigate the frequency and origin of interspecific brood-mixing. The main host species were a piscivore Lepidiolamprologus elongatus and a scale-eater Perissodus microlepis; more than half of their schools included heterospecific young, accounting for 20–40% of the total young. Most of the foreign young belonged to four biparental mouth-brooders whose parents have a habit of carrying their young in their mouths. Many of these young were smaller than the largest young brooded by their own parents. We concluded that adoption of young before independence results from farming-out, a behavior by which parents actively transfer their young to foster parents. 相似文献